##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706572
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192993778411825	32.0	32.0	32.0	32.0	32.0
2	31.32546152409096	32.0	32.0	32.0	32.0	32.0
3	31.392688926252386	32.0	32.0	32.0	32.0	32.0
4	31.49575414819721	32.0	32.0	32.0	32.0	32.0
5	31.416390686299486	32.0	32.0	32.0	32.0	32.0
6	34.95288236726052	36.0	36.0	36.0	36.0	36.0
7	34.97490843112946	36.0	36.0	36.0	36.0	36.0
8	34.91721438154923	36.0	36.0	36.0	36.0	36.0
9	35.01509117259104	36.0	36.0	36.0	36.0	36.0
10	34.871571474669246	36.0	36.0	36.0	32.0	36.0
11	35.04472438760664	36.0	36.0	36.0	36.0	36.0
12	34.940168871679035	36.0	36.0	36.0	36.0	36.0
13	34.99887909512406	36.0	36.0	36.0	36.0	36.0
14	34.945965874673774	36.0	36.0	36.0	32.0	36.0
15	34.90988462605368	36.0	36.0	36.0	32.0	36.0
16	34.91701058066269	36.0	36.0	36.0	32.0	36.0
17	34.879120599174605	36.0	36.0	36.0	32.0	36.0
18	34.882705513380095	36.0	36.0	36.0	32.0	36.0
19	34.87185311617217	36.0	36.0	36.0	32.0	36.0
20	34.85279207214551	36.0	36.0	36.0	32.0	36.0
21	34.83913882803168	36.0	36.0	36.0	32.0	36.0
22	34.81915501887989	36.0	36.0	36.0	32.0	36.0
23	34.75150869267392	36.0	36.0	36.0	32.0	36.0
24	34.72646382817321	36.0	36.0	36.0	32.0	36.0
25	34.701485482017404	36.0	36.0	36.0	32.0	36.0
26	34.638707732545306	36.0	36.0	36.0	32.0	36.0
27	34.62640042345296	36.0	36.0	36.0	32.0	36.0
28	34.57983899729964	36.0	36.0	36.0	32.0	36.0
29	34.54958730320477	36.0	36.0	36.0	32.0	36.0
30	34.52076646116744	36.0	36.0	36.0	32.0	36.0
31	34.51024382511619	36.0	36.0	36.0	32.0	36.0
32	34.48002326726788	36.0	36.0	36.0	32.0	36.0
33	34.45106089683712	36.0	36.0	36.0	32.0	36.0
34	34.42743131627067	36.0	36.0	36.0	32.0	36.0
35	34.36942731950884	36.0	36.0	36.0	32.0	36.0
36	34.3391275057602	36.0	36.0	36.0	32.0	36.0
37	34.333473446442824	36.0	36.0	36.0	32.0	36.0
38	33.87107187944045	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	7.0
21	9.0
22	56.0
23	173.0
24	456.0
25	1166.0
26	2596.0
27	4734.0
28	8299.0
29	13098.0
30	19695.0
31	28444.0
32	40489.0
33	64818.0
34	159568.0
35	362960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04312748581054	18.67008251829415	11.7565214929725	26.530268502922816
2	15.834904298500366	21.070747213305935	36.29877776079437	26.79557072739933
3	17.661045158879773	25.095248608775893	29.20707302298987	28.036633209354463
4	11.818062162188937	15.96640677299238	36.38445393315038	35.8310771316683
5	13.84459050174646	37.199039871379	33.36305429595286	15.593315330921689
6	33.69097710202401	36.39064266901039	16.55705246055233	13.361327768413275
7	29.59937387572905	30.954372467515554	21.387437037288645	18.058816619466747
8	28.308666909155754	32.45593798695371	19.666101016048152	19.56929408784238
9	27.646544305804028	13.74847143277701	18.772079304332074	39.83290495708689
10	16.04445715062103	26.045617690017096	31.218509754192098	26.691415405169778
11	37.87786575026821	20.939261238666504	22.170766021168127	19.01210698989716
12	24.7580214868711	24.22049588788671	28.2738748122977	22.747607812944487
13	29.383700457985878	18.857526196905624	25.71514297198304	26.043630373125453
14	23.736434503490088	20.092078372763144	24.810776537989053	31.36071058575772
15	25.448503478767908	26.969509122920236	22.50641123622221	25.07557616208964
16	26.09005168616928	25.326506003634446	23.672039084481128	24.91140322571514
17	23.910089842224146	25.862474029539804	25.34547080835357	24.881965319882475
18	25.491811167156353	23.66935004500603	26.825574746805703	24.013264041031913
19	25.689101747592602	24.921310213254984	25.484876275878467	23.904711763273948
20	25.600788030094595	23.671472970907423	25.462797846503964	25.264941152494014
21	27.44837737237641	23.98587542635549	24.11806331998245	24.44768388128565
22	25.72890763985502	24.23309193272863	24.941301015750717	25.096699411665636
23	23.783988043681322	23.663405852482125	25.85256704199997	26.700039061836584
24	24.6732109395787	25.246683989742024	25.256732505675288	24.82337256500399
25	25.01811563435857	24.038456095061793	25.40208216572409	25.541346104855556
26	24.509321059991056	25.031419303340634	26.038959936142387	24.42029970052592
27	25.51714474957966	24.549373595330696	24.792802432023915	25.14067922306573
28	24.12592064219924	24.096624264759996	26.29597549860453	25.481479594436234
29	24.287121482311782	24.534796170807788	26.18218667028979	24.99589567659064
30	24.343732839682296	24.67349399636555	26.52878970579078	24.453983458161378
31	24.98697938780478	24.479458568978107	24.801860249203195	25.731701794013915
32	24.314153405456203	24.683259455511966	24.94621921049801	26.056367928533824
33	24.02175574463749	24.167246933079714	25.85426538272108	25.956731939561717
34	25.06864127081175	24.214517416484092	26.206954139139395	24.50988717356476
35	26.303334975062697	24.094076753678323	25.667306375004955	23.935281896254025
36	24.45469110012851	25.247391631709153	25.01019004432669	25.287727223835642
37	25.860775688818695	24.89965636906076	24.937302921712153	24.302265020408395
38	24.584331108506987	24.42822529055779	25.69023397474001	25.297209626195205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	133.5
2	120.0
3	120.0
4	438.0
5	756.0
6	756.0
7	838.0
8	920.0
9	893.5
10	867.0
11	867.0
12	1053.0
13	1239.0
14	1598.0
15	1957.0
16	1957.0
17	2841.0
18	3725.0
19	3725.0
20	4285.0
21	4845.0
22	5134.5
23	5424.0
24	5424.0
25	5969.0
26	6514.0
27	6514.0
28	8727.0
29	10940.0
30	13438.5
31	15937.0
32	15937.0
33	21137.0
34	26337.0
35	26337.0
36	28428.5
37	30520.0
38	34810.5
39	39101.0
40	39101.0
41	40681.0
42	42261.0
43	48523.0
44	54785.0
45	54785.0
46	57029.5
47	59274.0
48	59274.0
49	62592.5
50	65911.0
51	64979.0
52	64047.0
53	64047.0
54	60417.0
55	56787.0
56	56787.0
57	55756.0
58	54725.0
59	48924.0
60	43123.0
61	43123.0
62	42663.0
63	42203.0
64	34703.0
65	27203.0
66	27203.0
67	22805.0
68	18407.0
69	18407.0
70	14826.0
71	11245.0
72	8757.5
73	6270.0
74	6270.0
75	4601.0
76	2932.0
77	2932.0
78	3188.5
79	3445.0
80	2620.5
81	1796.0
82	1796.0
83	1847.0
84	1898.0
85	1898.0
86	1184.0
87	470.0
88	410.5
89	351.0
90	351.0
91	209.5
92	68.0
93	41.5
94	15.0
95	15.0
96	10.0
97	5.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00877476039242993
2	0.0
3	0.0
4	1.415283934262892E-4
5	0.0
6	4.245851802788676E-4
7	4.245851802788676E-4
8	0.0015568123276891812
9	0.003962795015936097
10	5.661135737051568E-4
11	0.005378078950198989
12	1.415283934262892E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.830567868525784E-4
22	1.415283934262892E-4
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	706572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.48814457350831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04661794786043	38.81311983676242
2	12.202743074114872	11.833767407421183
3	3.3629025046407377	4.891827084948999
4	1.3708012415670239	2.658704351305862
5	0.7214183320633505	1.749011819153348
6	0.4362123969407243	1.2690677860571058
7	0.2825444677723732	0.9590039901254254
8	0.20795092390817269	0.8066523570123281
9	0.1546502280905867	0.6748832356184163
>10	0.9059408455815092	8.619142938734882
>50	0.1341482445447831	4.579266308487409
>100	0.15498963011696246	15.522362872725031
>500	0.014677048306529489	4.7810932930273875
>1k	0.004403114491958846	2.842096718620192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2556	0.36174657359759516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1865	0.2639504537400293	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1509	0.21356634568027036	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1441	0.20394241492728268	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1321	0.18695900771612803	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1320	0.18681747932270173	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1217	0.17224005479979393	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1182	0.16728656102987383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1145	0.1620500104731011	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1124	0.15907791421114903	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1099	0.1555397043754918	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1065	0.15072773899899797	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1058	0.14973704024501394	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1038	0.14690647237648818	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1028	0.14549118844222528	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	958	0.13558420090238504	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	942	0.1333197466075644	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	941	0.13317821821413814	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	934	0.13218751946015408	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	934	0.13218751946015408	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	919	0.13006459355875977	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	896	0.1268094405099551	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	847	0.11987454923206695	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.1176100949372463	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	814	0.1152041122489994	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	810	0.11463799867529424	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	795	0.1125150727738999	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	774	0.10954297651194782	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	773	0.10940144811852152	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	765	0.10826922097111123	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	751	0.10628782346314318	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	739	0.10458948274202771	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	734	0.10388184077489626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	4.245851802788675E-4	0.0
17	0.0	0.0	0.0	4.245851802788675E-4	0.0
18	0.0	0.0	0.0	4.245851802788675E-4	0.0
19	0.0	0.0	0.0	5.661135737051568E-4	0.0
20	0.0	0.0	0.0	5.661135737051568E-4	0.0
21	0.0	0.0	0.0	9.906987539840242E-4	0.0
22	0.0	0.0	0.0	0.0011322271474103135	0.0
23	0.0	0.0	0.0	0.0025475110816732052	0.0
24	0.0	0.0	0.0	0.0036797382290835188	0.0
25	0.0	0.0	0.0	0.004245851802788675	0.0
26	0.0	0.0	0.0	0.0052365505567726995	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	95	7.2759576E-11	21.897158	1
TTTAGAC	60	4.7117956E-6	21.335691	3
GTATAGA	40	0.004478932	20.002213	1
TCTAGCG	235	0.0	19.744068	28
CTAGCGG	235	0.0	19.744068	29
TAGCGGC	240	0.0	19.332735	30
TATACAC	130	1.2732926E-11	18.46358	3
TGTAGGA	185	0.0	18.162886	2
TACCGTC	215	0.0	17.861176	7
TCCATAC	45	0.008841359	17.779745	3
CATATAG	45	0.008841359	17.779745	1
CTAAGCT	45	0.008841359	17.779745	4
TAACTTT	90	4.3557884E-7	17.779745	6
TCTATAC	45	0.008844964	17.778486	2
CCGTCGT	200	0.0	17.600702	9
ACCGTCG	200	0.0	17.600702	8
CGTCGTA	200	0.0	17.599457	10
CATTAGT	55	0.0013635771	17.45524	2
CCCTAGA	65	2.097188E-4	17.232674	1
AGCGTAT	140	4.0017767E-11	17.14354	8
>>END_MODULE