FastQCFastQC Report
Thu 2 Feb 2017
SRR4063081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299656
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20940.16111955779067694No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19010.14626947438399082No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16740.12880331410773313No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14390.11072160633275266No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13960.10741303852711795No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13830.10641277384169351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT13440.10341197978542013No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13010.10010341197978542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATC350.002065962222.8585193
AAGACGG3800.018.1028695
AGTGCGT450.00885173717.7774810
GTATCAA16800.017.1610581
GATATAC3850.017.0570531
AACGAAT2000.016.7990731
CGCAAGA4250.016.5727182
CGCTTCG2800.016.57051332
GACGGAC4250.016.5631527
CAAGACG4250.016.1860964
GTTATTC1407.421477E-1016.0009633
CGGACCA4400.015.9997319
TATATCA3850.015.7901194
TAGCGGC3450.015.76724230
CTAGCGG3350.015.76032229
ACGGACC4700.015.6581078
GCGCAAG4400.015.6529651
TCTAGCG3500.015.54199728
TAAACGC2900.015.44742228
CGGTCCA3850.015.37636510
Produced by FastQC (version 0.11.5)