##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063081_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299656
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255369113057608	32.0	32.0	32.0	32.0	32.0
2	30.859619006875665	32.0	32.0	32.0	32.0	32.0
3	30.900837606258886	32.0	32.0	32.0	32.0	32.0
4	30.975875154656308	32.0	32.0	32.0	32.0	32.0
5	30.861364084034545	32.0	32.0	32.0	32.0	32.0
6	34.51869879414245	36.0	36.0	36.0	32.0	36.0
7	34.460790393765734	36.0	36.0	36.0	32.0	36.0
8	34.43987562862788	36.0	36.0	36.0	32.0	36.0
9	34.60227398634716	36.0	36.0	36.0	32.0	36.0
10	34.29916300928861	36.0	36.0	36.0	32.0	36.0
11	34.57220218273143	36.0	36.0	36.0	32.0	36.0
12	34.38686929464412	36.0	36.0	36.0	32.0	36.0
13	34.47885979059074	36.0	36.0	36.0	32.0	36.0
14	34.376449614359494	36.0	36.0	36.0	32.0	36.0
15	34.31394615190481	36.0	36.0	36.0	32.0	36.0
16	34.33976221400124	36.0	36.0	36.0	32.0	36.0
17	34.25207439507069	36.0	36.0	36.0	32.0	36.0
18	34.28337190764325	36.0	36.0	36.0	32.0	36.0
19	34.276753233163234	36.0	36.0	36.0	32.0	36.0
20	34.264907021550314	36.0	36.0	36.0	32.0	36.0
21	34.250228522008904	36.0	36.0	36.0	32.0	36.0
22	34.21828006795644	36.0	36.0	36.0	32.0	36.0
23	34.15751168001379	36.0	36.0	36.0	32.0	36.0
24	34.1453723139046	36.0	36.0	36.0	32.0	36.0
25	34.11732335325655	36.0	36.0	36.0	32.0	36.0
26	34.066711499042825	36.0	36.0	36.0	32.0	36.0
27	34.07443585071742	36.0	36.0	36.0	32.0	36.0
28	34.061090011510736	36.0	36.0	36.0	32.0	36.0
29	34.02754498113347	36.0	36.0	36.0	32.0	36.0
30	33.99243876841256	36.0	36.0	36.0	32.0	36.0
31	34.022736016299696	36.0	36.0	36.0	32.0	36.0
32	33.97485026807094	36.0	36.0	36.0	32.0	36.0
33	33.942487858325585	36.0	36.0	36.0	32.0	36.0
34	33.952483580270474	36.0	36.0	36.0	32.0	36.0
35	33.91835531863816	36.0	36.0	36.0	32.0	36.0
36	33.86899379528121	36.0	36.0	36.0	32.0	36.0
37	33.88040527647316	36.0	36.0	36.0	32.0	36.0
38	33.26936897148168	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	4.0
10	14.0
11	15.0
12	8.0
13	6.0
14	82.0
15	232.0
16	334.0
17	406.0
18	489.0
19	717.0
20	955.0
21	1418.0
22	2185.0
23	3497.0
24	5318.0
25	8264.0
26	12316.0
27	17782.0
28	25323.0
29	34338.0
30	46925.0
31	63572.0
32	87271.0
33	131502.0
34	288222.0
35	568455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13756206162059	16.78254070356404	11.043097438188743	26.03679979662662
2	16.923612255172912	18.900551048593588	37.822734488189944	26.353102208043556
3	19.742207549855905	22.634638840472647	27.923539528816967	29.699614080854488
4	12.572242437579687	14.630695043231967	35.84486814192181	36.95219437726654
5	14.690063663098025	36.317666086512254	33.829696211453204	15.162574038936516
6	35.277574731658525	35.171238410341246	16.24044935174855	13.310737506251682
7	31.069298337406202	29.731713622681692	20.221273937103355	18.977714102808743
8	28.191073128206156	33.63538924740449	18.83862489025883	19.334912734130523
9	27.12787730869494	14.109700135113092	18.287895528956984	40.474527027234984
10	15.725423903567663	26.641849671487723	31.734848001034127	25.89787842391049
11	38.604349107394995	20.73031948495833	21.57902714864234	19.08630425900434
12	24.66299738615702	23.24935231017706	28.828141170604617	23.259509133061304
13	29.89854309609779	18.98053665038308	25.026010644356	26.094909609163135
14	23.226492059815516	19.495601868061428	24.84192349689772	32.43598257522533
15	25.280003447853904	27.32285688558743	21.896488961095432	25.500650705463237
16	25.87706135389493	25.54231979746212	23.769036021269553	24.811582827373392
17	24.107009553654844	25.895068331642808	25.026743153367942	24.971178961334402
18	25.039205154919998	24.7983570000631	26.155570979413255	24.00686686560365
19	25.517024328383936	24.758455221491715	25.07818078845633	24.64633966166802
20	25.66247065368696	24.46437928940947	24.94376973253399	24.92938032436958
21	26.739444753521752	24.147812309792414	24.308096465976746	24.80464647070909
22	25.751782354593338	24.284191774813493	24.98728401094229	24.97674185965088
23	24.520306234499166	24.1021540914502	25.343067131348562	26.034472542702076
24	24.940880425707107	24.869313612700417	25.166969991958354	25.022835969634123
25	25.07420441401792	24.419543330221764	25.27590497833316	25.23034727742716
26	24.49712411480146	25.521653406965843	25.640859887365654	24.340362590867045
27	25.23858699248803	24.680152609489923	24.993343726866566	25.08791667115548
28	24.515939392680245	24.74616779741747	25.566356489293838	25.171536320608446
29	24.45719966972192	24.76685460915683	25.471581233248507	25.304364487872743
30	24.398589732935676	25.1521223257403	25.622185698500783	24.827102242823244
31	25.205086278206334	24.812881134216955	24.698459111632648	25.28357347594406
32	24.848102434670196	24.903813598448707	24.65973098587214	25.588352981008956
33	24.38991733209597	24.39861273669007	25.429518359231018	25.781951571982948
34	25.219970911665346	24.920853565629596	25.429591609015844	24.429583913689218
35	25.618572250901156	24.53641711378097	25.618264426939525	24.22674620837835
36	24.342256639381414	25.20906423410577	25.15196451506409	25.296714611448724
37	25.42746552573258	24.899578305836002	24.9088124846097	24.764143683821718
38	24.701343291506646	24.70511377233529	25.332783407422	25.260759528736067
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	41.0
2	68.0
3	68.0
4	207.5
5	347.0
6	347.0
7	430.0
8	513.0
9	538.0
10	563.0
11	563.0
12	738.0
13	913.0
14	1437.5
15	1962.0
16	1962.0
17	2878.5
18	3795.0
19	3795.0
20	4853.5
21	5912.0
22	7008.0
23	8104.0
24	8104.0
25	10547.0
26	12990.0
27	12990.0
28	16876.0
29	20762.0
30	25336.0
31	29910.0
32	29910.0
33	37292.0
34	44674.0
35	44674.0
36	50648.5
37	56623.0
38	64490.5
39	72358.0
40	72358.0
41	76597.5
42	80837.0
43	90046.5
44	99256.0
45	99256.0
46	102538.5
47	105821.0
48	105821.0
49	113039.5
50	120258.0
51	122570.0
52	124882.0
53	124882.0
54	119865.0
55	114848.0
56	114848.0
57	110250.5
58	105653.0
59	95365.5
60	85078.0
61	85078.0
62	79130.5
63	73183.0
64	61118.0
65	49053.0
66	49053.0
67	40880.5
68	32708.0
69	32708.0
70	25951.0
71	19194.0
72	15205.0
73	11216.0
74	11216.0
75	8333.0
76	5450.0
77	5450.0
78	5019.0
79	4588.0
80	3629.0
81	2670.0
82	2670.0
83	2632.0
84	2594.0
85	2594.0
86	1743.5
87	893.0
88	782.0
89	671.0
90	671.0
91	480.5
92	290.0
93	248.5
94	207.0
95	207.0
96	189.5
97	172.0
98	172.0
99	399.0
100	626.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11933927131487101
2	0.05216765051675212
3	0.012387893411795121
4	0.003000794056273352
5	1.5388687468068473E-4
6	4.616606240420542E-4
7	0.0
8	5.386040613823966E-4
9	3.0777374936136946E-4
10	6.924909360630812E-4
11	0.0025391334322312983
12	0.0028469071815926676
13	0.014465366219984364
14	0.00977181654222348
15	0.022852200890081685
16	0.011233741851689984
17	0.01985140683380833
18	0.006309361861908074
19	0.008002117483395606
20	0.006847965923290471
21	0.006847965923290471
22	0.008540721544778002
23	0.009925703416904165
24	0.013157327785198547
25	0.015773404654770184
26	0.017696990588278744
27	0.010002646854244509
28	0.005924644675206362
29	0.011156798414349644
30	0.0033855112429750644
31	0.006232418424567732
32	0.0073865699846728675
33	0.009156269043500741
34	0.012772610598496832
35	0.01631200871615258
36	0.013465101534559916
37	0.010464307478286562
38	0.006694079048609787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1299656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.079038421950834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37749242033877	46.68247470540916
2	12.115685326239815	14.073347071418961
3	3.4149902453112975	5.950180490040664
4	1.3812792876822462	3.2089349128296822
5	0.7398941662822542	2.148617085584216
6	0.4339032117520406	1.5120408784052781
7	0.28764601628857805	1.1694342822361794
8	0.21170829792578885	0.9836651495582164
9	0.14761012624137648	0.7715748774097725
>10	0.749068428871904	7.806229867860807
>50	0.06334816618298156	2.5577029628121886
>100	0.06698672795208026	8.169473619141247
>500	0.0078572712427412	3.0448116995134478
>1k	0.002530307688340386	1.9215123977801298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2094	0.16111955779067694	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1901	0.14626947438399082	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1674	0.12880331410773313	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1439	0.11072160633275266	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1396	0.10741303852711795	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1383	0.10641277384169351	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1344	0.10341197978542013	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1301	0.10010341197978542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.694343734034237E-5
6	0.0	0.0	0.0	0.0	7.694343734034237E-5
7	0.0	0.0	0.0	0.0	7.694343734034237E-5
8	0.0	0.0	0.0	0.0	7.694343734034237E-5
9	0.0	0.0	0.0	7.694343734034237E-5	7.694343734034237E-5
10	0.0	0.0	0.0	7.694343734034237E-5	7.694343734034237E-5
11	0.0	0.0	0.0	7.694343734034237E-5	7.694343734034237E-5
12	0.0	0.0	0.0	7.694343734034237E-5	1.5388687468068473E-4
13	0.0	0.0	0.0	7.694343734034237E-5	1.5388687468068473E-4
14	0.0	0.0	0.0	7.694343734034237E-5	1.5388687468068473E-4
15	0.0	0.0	0.0	7.694343734034237E-5	1.5388687468068473E-4
16	0.0	0.0	0.0	1.5388687468068473E-4	1.5388687468068473E-4
17	0.0	0.0	0.0	1.5388687468068473E-4	1.5388687468068473E-4
18	0.0	0.0	0.0	1.5388687468068473E-4	1.5388687468068473E-4
19	0.0	0.0	0.0	1.5388687468068473E-4	1.5388687468068473E-4
20	0.0	0.0	0.0	1.5388687468068473E-4	2.308303120210271E-4
21	0.0	0.0	0.0	1.5388687468068473E-4	2.308303120210271E-4
22	0.0	0.0	0.0	3.0777374936136946E-4	3.0777374936136946E-4
23	0.0	0.0	0.0	3.0777374936136946E-4	3.0777374936136946E-4
24	0.0	0.0	0.0	4.616606240420542E-4	3.0777374936136946E-4
25	0.0	0.0	0.0	6.155474987227389E-4	3.0777374936136946E-4
26	0.0	0.0	0.0	9.233212480841084E-4	3.0777374936136946E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATC	35	0.0020659622	22.858519	3
AAGACGG	380	0.0	18.102869	5
AGTGCGT	45	0.008851737	17.77748	10
GTATCAA	1680	0.0	17.161058	1
GATATAC	385	0.0	17.057053	1
AACGAAT	200	0.0	16.79907	31
CGCAAGA	425	0.0	16.572718	2
CGCTTCG	280	0.0	16.570513	32
GACGGAC	425	0.0	16.563152	7
CAAGACG	425	0.0	16.186096	4
GTTATTC	140	7.421477E-10	16.000963	3
CGGACCA	440	0.0	15.999731	9
TATATCA	385	0.0	15.790119	4
TAGCGGC	345	0.0	15.767242	30
CTAGCGG	335	0.0	15.760322	29
ACGGACC	470	0.0	15.658107	8
GCGCAAG	440	0.0	15.652965	1
TCTAGCG	350	0.0	15.541997	28
TAAACGC	290	0.0	15.447422	28
CGGTCCA	385	0.0	15.376365	10
>>END_MODULE