FastQCFastQC Report
Thu 2 Feb 2017
SRR4063081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299656
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19190.147654456256117No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18680.14373034095175954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT17720.13634377096708666No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14880.11449183476242944No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14350.1104138325833913No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14300.11002911539668958No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14240.10956745477264754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13170.1013345069772309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTTT1600.019.99944728
TCTAGCG3400.018.35243428
TCACGTT1850.018.16166127
GTCCTAC3650.017.9734881
TACTATA904.3673208E-717.777972
CTAGCGG3550.017.57697929
CAAGACG4150.017.3495864
ACGTTTT1850.017.2968229
GGACCGA652.0998776E-417.231626
CGCAAGA3750.017.066852
TAGCGGC3700.016.86439930
GACGGAC4050.016.593417
AAGACGG4250.016.5655215
GTATCAA17250.016.3254411
CGCCGGT3950.016.2033317
GCGCAAG4050.016.1983281
GTATTAC1001.3140161E-616.000791
CGGTCCA4100.016.0007910
TCTATAC908.58165E-616.0001743
CGTGATT1203.111927E-815.999557528
Produced by FastQC (version 0.11.5)