##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299656
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159848452205814	32.0	32.0	32.0	32.0	32.0
2	31.164356568199587	32.0	32.0	32.0	32.0	32.0
3	31.301637510233476	32.0	32.0	32.0	32.0	32.0
4	31.41971337030722	32.0	32.0	32.0	32.0	32.0
5	31.313444480693352	32.0	32.0	32.0	32.0	32.0
6	34.85033885889805	36.0	36.0	36.0	32.0	36.0
7	34.8787117514173	36.0	36.0	36.0	32.0	36.0
8	34.80252235976289	36.0	36.0	36.0	32.0	36.0
9	34.95020682395957	36.0	36.0	36.0	32.0	36.0
10	34.75462968662477	36.0	36.0	36.0	32.0	36.0
11	34.962597025674484	36.0	36.0	36.0	36.0	36.0
12	34.85002954627994	36.0	36.0	36.0	32.0	36.0
13	34.92435767618508	36.0	36.0	36.0	32.0	36.0
14	34.86168724647138	36.0	36.0	36.0	32.0	36.0
15	34.81538037757684	36.0	36.0	36.0	32.0	36.0
16	34.846110047581824	36.0	36.0	36.0	32.0	36.0
17	34.80603867484935	36.0	36.0	36.0	32.0	36.0
18	34.80469447299901	36.0	36.0	36.0	32.0	36.0
19	34.80424973993118	36.0	36.0	36.0	32.0	36.0
20	34.78813162867713	36.0	36.0	36.0	32.0	36.0
21	34.78709443114178	36.0	36.0	36.0	32.0	36.0
22	34.76469311879451	36.0	36.0	36.0	32.0	36.0
23	34.70445794887262	36.0	36.0	36.0	32.0	36.0
24	34.68067857956259	36.0	36.0	36.0	32.0	36.0
25	34.66136039074955	36.0	36.0	36.0	32.0	36.0
26	34.59899080987584	36.0	36.0	36.0	32.0	36.0
27	34.58452313535274	36.0	36.0	36.0	32.0	36.0
28	34.557600626627355	36.0	36.0	36.0	32.0	36.0
29	34.519295105781836	36.0	36.0	36.0	32.0	36.0
30	34.50150809137187	36.0	36.0	36.0	32.0	36.0
31	34.49856885206547	36.0	36.0	36.0	32.0	36.0
32	34.47380152901999	36.0	36.0	36.0	32.0	36.0
33	34.44467920742104	36.0	36.0	36.0	32.0	36.0
34	34.43939011553827	36.0	36.0	36.0	32.0	36.0
35	34.407024628055424	36.0	36.0	36.0	32.0	36.0
36	34.38205186603224	36.0	36.0	36.0	32.0	36.0
37	34.37768070935694	36.0	36.0	36.0	32.0	36.0
38	33.9264112965277	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	17.0
22	82.0
23	257.0
24	748.0
25	1894.0
26	4232.0
27	8311.0
28	14657.0
29	24204.0
30	37221.0
31	54922.0
32	81484.0
33	132914.0
34	317919.0
35	620792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.322773340802534	18.113606231036837	11.811314453595411	26.752305974565218
2	15.930677040693844	19.93404408551186	36.89430126125682	27.24097761253747
3	18.751346510153454	23.392189933336205	28.250937171066802	29.605526385443532
4	12.10407701132533	15.596009088579798	35.88565563269581	36.41425826739907
5	14.199680530848163	36.912229082157126	33.38860436915614	15.499486017838565
6	35.430606252731494	35.51239712662427	15.897514419200157	13.159482201444076
7	30.83575770959345	29.948516789559008	20.10738300476976	19.108342496077782
8	28.018876127146857	33.954945673041045	18.64075264440445	19.38542555540765
9	26.949786509755764	13.955621422432134	17.968345425599285	41.12624664221282
10	15.427142019546983	26.931807164764344	31.616419341385832	26.024631474302844
11	38.748088830614414	20.675563270767785	21.495586708802804	19.080761189814996
12	24.619263757325992	23.306451799057132	28.62364031014432	23.45064413347255
13	29.79045913095131	18.875375004520446	25.05041738128159	26.283748483246654
14	23.10151540216442	19.500944481420067	24.716405507615484	32.68113460880003
15	25.216364945801047	27.437414208067366	21.746215921751602	25.600004924379988
16	25.95504191496974	25.635341686832273	23.571409335554435	24.838207062643548
17	24.030512689511685	26.078746991511597	24.844343426260487	25.046396892716228
18	25.01161845903839	24.987765993462887	25.898853235009877	24.10176231248884
19	25.695107012932656	24.829724173165822	24.812411899764246	24.66275691413728
20	25.751198778753764	24.46747447016749	24.700074481247345	25.0812522698314
21	26.789032474002717	24.296370959985538	24.10478165359268	24.809814912419064
22	25.65371144364355	24.48532534763045	24.922594902035616	24.938368306690386
23	24.5119666372999	24.130557724934697	25.298867776448365	26.05860786131704
24	24.953833937595796	24.93929162793847	24.99076678751916	25.116107646946578
25	25.040722345545547	24.517198795064843	25.132592880418265	25.309485978971342
26	24.58665985460768	25.50567227020073	25.451273260001106	24.45639461519048
27	25.283344644036028	24.75851265029	24.798984960612593	25.15915774506138
28	24.476015191712268	24.82672337910955	25.408261878527856	25.288999550650328
29	24.350135728223467	24.72569664588168	25.36425023236918	25.559917393525673
30	24.440698153973052	25.09871843010766	25.458121225924398	25.00246218999489
31	25.127264445360925	24.812488843201585	24.702305840930215	25.357940870507274
32	24.921979354536894	24.978917498168745	24.553112515927292	25.545990631367072
33	24.47570741796291	24.55326640280197	25.189434742731926	25.7815914365032
34	25.27838135629736	24.789790529186185	25.362480533310354	24.569347581206102
35	25.66279076924971	24.552881685615272	25.36024917362748	24.424078371507537
36	24.470706094535785	25.23806299513102	24.96637571788227	25.324855192450922
37	25.550145576983446	24.971761758496093	24.783865884510977	24.69422678000948
38	24.764745078305456	24.662717923385763	25.15592611572003	25.416610882588753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	21.0
2	32.0
3	32.0
4	123.0
5	214.0
6	214.0
7	291.0
8	368.0
9	405.0
10	442.0
11	442.0
12	644.0
13	846.0
14	1284.0
15	1722.0
16	1722.0
17	2656.0
18	3590.0
19	3590.0
20	4505.0
21	5420.0
22	6693.5
23	7967.0
24	7967.0
25	10587.5
26	13208.0
27	13208.0
28	17096.5
29	20985.0
30	25563.0
31	30141.0
32	30141.0
33	37649.0
34	45157.0
35	45157.0
36	51019.0
37	56881.0
38	65133.0
39	73385.0
40	73385.0
41	77236.0
42	81087.0
43	90439.5
44	99792.0
45	99792.0
46	102446.5
47	105101.0
48	105101.0
49	112591.0
50	120081.0
51	121829.5
52	123578.0
53	123578.0
54	119055.0
55	114532.0
56	114532.0
57	110440.5
58	106349.0
59	95928.5
60	85508.0
61	85508.0
62	79710.0
63	73912.0
64	61702.0
65	49492.0
66	49492.0
67	41312.5
68	33133.0
69	33133.0
70	26254.0
71	19375.0
72	15270.0
73	11165.0
74	11165.0
75	8305.0
76	5445.0
77	5445.0
78	5145.5
79	4846.0
80	3705.5
81	2565.0
82	2565.0
83	2393.0
84	2221.0
85	2221.0
86	1406.0
87	591.0
88	491.5
89	392.0
90	392.0
91	233.0
92	74.0
93	51.5
94	29.0
95	29.0
96	19.0
97	9.0
98	9.0
99	10.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00746351342201321
2	0.0
3	0.0
4	2.308303120210271E-4
5	0.0
6	0.0
7	6.924909360630812E-4
8	0.001923585933508559
9	0.0050013234271222545
10	0.0013849818721261625
11	0.004539662803080199
12	2.308303120210271E-4
13	3.8471718670171184E-4
14	7.694343734034237E-5
15	0.0
16	7.694343734034237E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	7.694343734034237E-5
22	0.0
23	7.694343734034237E-5
24	0.0
25	7.694343734034237E-5
26	0.0
27	1.5388687468068473E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.5388687468068473E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1299656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.49554743226718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17767649341741	44.731861779105685
2	12.88119109306065	14.554598847642108
3	3.6055652212163594	6.110951429260853
4	1.4745133720075168	3.332137605910515
5	0.7667819465746618	2.165988291645748
6	0.4636243641825007	1.5715627354456312
7	0.2947549497844033	1.1656639572508203
8	0.21095476811204164	0.9534404086349417
9	0.15538154550657834	0.790052892683929
>10	0.8188825873657866	8.167871934924676
>50	0.06777711658190573	2.6456120807566577
>100	0.07111292248890053	8.337326134780081
>500	0.009180261860249589	3.5346744247920765
>1k	0.0026033578409663015	1.9382574771663263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1919	0.147654456256117	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1868	0.14373034095175954	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1772	0.13634377096708666	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1488	0.11449183476242944	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1435	0.1104138325833913	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1430	0.11002911539668958	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1424	0.10956745477264754	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1317	0.1013345069772309	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	7.694343734034237E-5	0.0
17	0.0	0.0	0.0	7.694343734034237E-5	0.0
18	0.0	0.0	0.0	7.694343734034237E-5	0.0
19	0.0	0.0	0.0	7.694343734034237E-5	0.0
20	0.0	0.0	0.0	1.5388687468068473E-4	0.0
21	0.0	0.0	0.0	1.5388687468068473E-4	0.0
22	0.0	0.0	0.0	3.0777374936136946E-4	0.0
23	0.0	0.0	0.0	4.616606240420542E-4	0.0
24	0.0	0.0	0.0	6.155474987227389E-4	0.0
25	0.0	0.0	0.0	7.694343734034237E-4	0.0
26	0.0	0.0	0.0	0.0010002646854244508	7.694343734034237E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTTT	160	0.0	19.999447	28
TCTAGCG	340	0.0	18.352434	28
TCACGTT	185	0.0	18.161661	27
GTCCTAC	365	0.0	17.973488	1
TACTATA	90	4.3673208E-7	17.77797	2
CTAGCGG	355	0.0	17.576979	29
CAAGACG	415	0.0	17.349586	4
ACGTTTT	185	0.0	17.29682	29
GGACCGA	65	2.0998776E-4	17.23162	6
CGCAAGA	375	0.0	17.06685	2
TAGCGGC	370	0.0	16.864399	30
GACGGAC	405	0.0	16.59341	7
AAGACGG	425	0.0	16.565521	5
GTATCAA	1725	0.0	16.325441	1
CGCCGGT	395	0.0	16.203331	7
GCGCAAG	405	0.0	16.198328	1
GTATTAC	100	1.3140161E-6	16.00079	1
CGGTCCA	410	0.0	16.00079	10
TCTATAC	90	8.58165E-6	16.000174	3
CGTGATT	120	3.111927E-8	15.9995575	28
>>END_MODULE