FastQCFastQC Report
Thu 2 Feb 2017
SRR4063080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083666
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30220.2788682121613117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19860.18326679991805594No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15900.14672417516098135No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13810.1274377898725253No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12230.11285765171187433No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12090.11156574073561412No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11470.10584442069789032No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11170.10307604003447557No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11170.10307604003447557No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11140.1027992019681341No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10990.10141501163642673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG400.00448268120.0004915
CGTTATT1550.018.5853882
ACCGTCT550.001365619617.4525578
TAGCGGC2700.017.18560630
CTAGCGG2700.017.18560629
TCTAGCG2800.016.57030528
AAGACGG4100.015.6065375
AACCGCG1357.918061E-915.4056527
CGCAAGA4200.015.2419852
CGCGCCA859.429421E-515.05919210
AGCGGCG3200.015.00036731
CGTTTAT2350.014.9790931
GTATAAT1501.9499566E-914.9447361
CCTATAC756.247154E-414.9323223
CAAGACG4400.014.9060164
GCGCAAG4300.014.8950831
GTATCAA22150.014.53027251
CGAGCCG2550.014.43172615
TACGCTA2800.014.2860649
GTCTTAC901.5241388E-414.233081
Produced by FastQC (version 0.11.5)