##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063080_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083666
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244579049264257	32.0	32.0	32.0	32.0	32.0
2	30.927528408199574	32.0	32.0	32.0	32.0	32.0
3	30.97566132000081	32.0	32.0	32.0	32.0	32.0
4	31.03280069689369	32.0	32.0	32.0	32.0	32.0
5	30.975465687767265	32.0	32.0	32.0	32.0	32.0
6	34.574494355271824	36.0	36.0	36.0	32.0	36.0
7	34.51667303394219	36.0	36.0	36.0	32.0	36.0
8	34.50682128995465	36.0	36.0	36.0	32.0	36.0
9	34.62208097328882	36.0	36.0	36.0	32.0	36.0
10	34.38646409502559	36.0	36.0	36.0	32.0	36.0
11	34.610964079338096	36.0	36.0	36.0	32.0	36.0
12	34.46359671706965	36.0	36.0	36.0	32.0	36.0
13	34.53228300970964	36.0	36.0	36.0	32.0	36.0
14	34.44009777920503	36.0	36.0	36.0	32.0	36.0
15	34.40208975828346	36.0	36.0	36.0	32.0	36.0
16	34.41120788139519	36.0	36.0	36.0	32.0	36.0
17	34.33845483756065	36.0	36.0	36.0	32.0	36.0
18	34.371058056633686	36.0	36.0	36.0	32.0	36.0
19	34.34924413979953	36.0	36.0	36.0	32.0	36.0
20	34.32496636417494	36.0	36.0	36.0	32.0	36.0
21	34.31687254190867	36.0	36.0	36.0	32.0	36.0
22	34.29587898854444	36.0	36.0	36.0	32.0	36.0
23	34.25441879693559	36.0	36.0	36.0	32.0	36.0
24	34.23521361747992	36.0	36.0	36.0	32.0	36.0
25	34.21706503664413	36.0	36.0	36.0	32.0	36.0
26	34.169948120546366	36.0	36.0	36.0	32.0	36.0
27	34.1767537230106	36.0	36.0	36.0	32.0	36.0
28	34.160245869114654	36.0	36.0	36.0	32.0	36.0
29	34.12819724896786	36.0	36.0	36.0	32.0	36.0
30	34.10561003113506	36.0	36.0	36.0	32.0	36.0
31	34.11792932508725	36.0	36.0	36.0	32.0	36.0
32	34.07211077952063	36.0	36.0	36.0	32.0	36.0
33	34.03660168354456	36.0	36.0	36.0	32.0	36.0
34	34.03373271838371	36.0	36.0	36.0	32.0	36.0
35	34.007312216125634	36.0	36.0	36.0	32.0	36.0
36	33.969593029586605	36.0	36.0	36.0	32.0	36.0
37	33.96918054086776	36.0	36.0	36.0	32.0	36.0
38	33.34588793964192	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	1.0
9	4.0
10	13.0
11	7.0
12	6.0
13	12.0
14	103.0
15	239.0
16	360.0
17	433.0
18	593.0
19	742.0
20	1039.0
21	1364.0
22	1889.0
23	2816.0
24	3988.0
25	6106.0
26	8783.0
27	12990.0
28	18752.0
29	26354.0
30	36221.0
31	50045.0
32	70375.0
33	108236.0
34	244734.0
35	487457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.54001714809449	17.21461727227035	11.305361736096739	25.940003843538424
2	17.00116243318779	19.483081934458713	37.23026979714202	26.285485835211475
3	19.34876243266266	23.248398531845243	28.315415163487728	29.08742387200437
4	12.594253653179777	15.042656958690346	36.02563570172889	36.33745368640099
5	14.683781426916989	36.374248499305594	33.67802780379545	15.263942269981959
6	34.56003809305854	35.42645215943717	16.683984489628685	13.329525257875611
7	30.499526606906556	30.39248255458785	20.668730033054462	18.439260805451127
8	28.42567924839964	32.610013648179645	19.316280644961846	19.648026458458872
9	27.343212182396353	14.175360952031168	18.695312745117942	39.786114120454535
10	15.806433752283928	26.476570141926437	31.576601517081006	26.140394588708638
11	37.765898052764804	21.270552278018787	21.871490413320302	19.092059255896103
12	24.550195314482618	23.92240205899208	28.562332220106917	22.965070406418388
13	29.36116564173702	19.20074869241866	25.442251836428426	25.995833829415897
14	23.476541086092617	19.71632239832994	25.1581370194748	31.648999496102643
15	25.100494836111498	27.129590571807544	22.41281755675939	25.35709703532157
16	25.89591428017683	25.85604459498122	23.623157642151117	24.624883482690834
17	24.258814342926605	25.752584608369876	25.141379300796636	24.847221747906882
18	25.079641934293097	24.811461794019934	26.171465485418977	23.937430786267996
19	25.300186327321455	25.04150604894714	25.698035303444055	23.960272320287345
20	25.589471372224754	24.258534991758935	25.200028054684488	24.951965581331823
21	26.9822710187312	24.045374970122545	24.701433102650164	24.270920908496084
22	25.70808935155064	24.137931034482758	25.44064378890442	24.713335825062178
23	24.312880406443664	24.05926841986258	25.92508848271184	25.702762690981917
24	24.547897819461852	25.015459245736476	25.566269863977098	24.870373070824574
25	24.69800565593319	24.521257743681378	25.622540296678064	25.15819630370737
26	24.526624843208182	25.533507531083778	25.855074978563707	24.084792647144333
27	25.299819393135092	24.724129243269232	25.18455216049924	24.791499203096436
28	24.36445350892169	24.784075231059575	25.994305990706863	24.857165269311874
29	24.299495819724402	25.080546098394063	25.967545298239752	24.65241278364178
30	24.302617882217447	25.25829992691192	26.071681690328006	24.367400500542622
31	24.84256066859113	25.147195623444986	24.975175158177617	25.035068549786267
32	24.589994490500498	24.862977732208794	24.979535485514262	25.56749229177645
33	24.355440216543663	24.474029803362775	25.55887073181311	25.61165924828045
34	25.000945984733193	24.842943461491963	25.66839589599521	24.48771465777964
35	25.65472811765955	24.52880342235328	25.623439612723992	24.19302884726318
36	24.29967180191455	25.393173184481288	25.310939074769358	24.996215938834805
37	25.36518513049577	25.139170881169477	25.109177156779506	24.386466831555246
38	24.35259363986294	24.802578089744028	25.717128109908426	25.12770016048461
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	62.5
2	101.0
3	101.0
4	322.0
5	543.0
6	543.0
7	725.5
8	908.0
9	894.0
10	880.0
11	880.0
12	1176.0
13	1472.0
14	2099.0
15	2726.0
16	2726.0
17	3971.0
18	5216.0
19	5216.0
20	6457.0
21	7698.0
22	8303.5
23	8909.0
24	8909.0
25	10481.5
26	12054.0
27	12054.0
28	15165.0
29	18276.0
30	21888.5
31	25501.0
32	25501.0
33	31736.0
34	37971.0
35	37971.0
36	42373.5
37	46776.0
38	53363.0
39	59950.0
40	59950.0
41	64037.5
42	68125.0
43	76707.0
44	85289.0
45	85289.0
46	86585.5
47	87882.0
48	87882.0
49	92818.5
50	97755.0
51	99638.5
52	101522.0
53	101522.0
54	95658.5
55	89795.0
56	89795.0
57	87200.0
58	84605.0
59	75520.5
60	66436.0
61	66436.0
62	63220.0
63	60004.0
64	50487.0
65	40970.0
66	40970.0
67	34452.5
68	27935.0
69	27935.0
70	22621.0
71	17307.0
72	13625.5
73	9944.0
74	9944.0
75	7642.5
76	5341.0
77	5341.0
78	4891.0
79	4441.0
80	3451.5
81	2462.0
82	2462.0
83	2462.5
84	2463.0
85	2463.0
86	1649.5
87	836.0
88	677.5
89	519.0
90	519.0
91	381.0
92	243.0
93	209.5
94	176.0
95	176.0
96	151.5
97	127.0
98	127.0
99	305.5
100	484.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12273154274472023
2	0.05472165778016473
3	0.01190403685268339
4	0.0019378664643903195
5	9.227935544715808E-5
6	3.691174217886323E-4
7	0.0
8	4.6139677723579036E-4
9	3.691174217886323E-4
10	5.536761326829485E-4
11	0.0024915425970732682
12	0.0026761013079675843
13	0.01633344591414698
14	0.010335287810081705
15	0.025561381458862786
16	0.012549992340813498
17	0.02150108981918783
18	0.006090437459512433
19	0.008212862634797069
20	0.006274996170406749
21	0.006736392947642539
22	0.007474627791219803
23	0.011165802009106126
24	0.015872049136911186
25	0.01864042980032593
26	0.020578296264716252
27	0.008951097478374333
28	0.006551834236748223
29	0.011534919430894758
30	0.003875732928780639
31	0.0068286723030896975
32	0.0076591865021141195
33	0.008951097478374333
34	0.012826830407154972
35	0.018548150444878772
36	0.016056607847805503
37	0.009781611677398756
38	0.006736392947642539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1083666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.18166334327477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89071599898674	47.87247121848483
2	12.433240828862388	14.716397459991848
3	3.2957382698296263	5.851418182578114
4	1.2260426660674677	2.902369772307837
5	0.6318847890211292	1.869799642779234
6	0.36042808862060105	1.279844028012265
7	0.21726548269337576	0.9000692857011418
8	0.12874672216910743	0.6095556134369797
9	0.10159967021358167	0.5411553730531138
>10	0.5591439327326687	6.254898645423318
>50	0.07158102333949956	2.966589104750182
>100	0.07406128492991773	9.34835563734536
>500	0.007359154083514676	3.109013976154165
>1k	0.002192088450408627	1.778062059981703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3022	0.2788682121613117	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1986	0.18326679991805594	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1590	0.14672417516098135	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1381	0.1274377898725253	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1223	0.11285765171187433	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1209	0.11156574073561412	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1147	0.10584442069789032	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1117	0.10307604003447557	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1117	0.10307604003447557	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1114	0.1027992019681341	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1099	0.10141501163642673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.227935544715808E-5
2	0.0	0.0	0.0	9.227935544715808E-5	9.227935544715808E-5
3	0.0	0.0	0.0	1.8455871089431616E-4	9.227935544715808E-5
4	0.0	0.0	0.0	1.8455871089431616E-4	9.227935544715808E-5
5	0.0	0.0	0.0	1.8455871089431616E-4	9.227935544715808E-5
6	0.0	0.0	0.0	1.8455871089431616E-4	1.8455871089431616E-4
7	0.0	0.0	0.0	2.768380663414742E-4	1.8455871089431616E-4
8	0.0	0.0	0.0	2.768380663414742E-4	1.8455871089431616E-4
9	0.0	0.0	0.0	3.691174217886323E-4	1.8455871089431616E-4
10	0.0	0.0	0.0	3.691174217886323E-4	1.8455871089431616E-4
11	0.0	0.0	0.0	3.691174217886323E-4	1.8455871089431616E-4
12	0.0	0.0	0.0	4.6139677723579036E-4	4.6139677723579036E-4
13	0.0	0.0	0.0	4.6139677723579036E-4	6.459554881301065E-4
14	0.0	0.0	0.0	4.6139677723579036E-4	6.459554881301065E-4
15	0.0	0.0	0.0	4.6139677723579036E-4	8.305141990244227E-4
16	0.0	0.0	0.0	4.6139677723579036E-4	8.305141990244227E-4
17	0.0	0.0	0.0	5.536761326829484E-4	8.305141990244227E-4
18	0.0	0.0	0.0	8.305141990244227E-4	8.305141990244227E-4
19	0.0	0.0	0.0	9.227935544715807E-4	8.305141990244227E-4
20	0.0	0.0	0.0	9.227935544715807E-4	9.227935544715807E-4
21	0.0	0.0	0.0	9.227935544715807E-4	9.227935544715807E-4
22	0.0	0.0	0.0	0.0013841903317073712	0.0010150729099187388
23	0.0	0.0	0.0	0.0023069838861789517	0.0010150729099187388
24	0.0	0.0	0.0	0.0029529393743090585	0.0010150729099187388
25	0.0	0.0	0.0	0.0032297774406505326	0.0010150729099187388
26	0.0	0.0	0.0	0.003691174217886323	0.0011996316208130549
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	40	0.004482681	20.00049	15
CGTTATT	155	0.0	18.585388	2
ACCGTCT	55	0.0013656196	17.452557	8
TAGCGGC	270	0.0	17.185606	30
CTAGCGG	270	0.0	17.185606	29
TCTAGCG	280	0.0	16.570305	28
AAGACGG	410	0.0	15.606537	5
AACCGCG	135	7.918061E-9	15.405652	7
CGCAAGA	420	0.0	15.241985	2
CGCGCCA	85	9.429421E-5	15.059192	10
AGCGGCG	320	0.0	15.000367	31
CGTTTAT	235	0.0	14.97909	31
GTATAAT	150	1.9499566E-9	14.944736	1
CCTATAC	75	6.247154E-4	14.932322	3
CAAGACG	440	0.0	14.906016	4
GCGCAAG	430	0.0	14.895083	1
GTATCAA	2215	0.0	14.5302725	1
CGAGCCG	255	0.0	14.431726	15
TACGCTA	280	0.0	14.286064	9
GTCTTAC	90	1.5241388E-4	14.23308	1
>>END_MODULE