FastQCFastQC Report
Thu 2 Feb 2017
SRR4063080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083666
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26900.2482314661528552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20030.18483554896065763No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16340.1507844668006563No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16080.14838520355903018No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12840.11848669239415095No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12410.11451868010992318No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12410.11451868010992318No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11770.10861280136130506No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11450.105659861986996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11130.10270692261268695No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT11100.10243008454634546No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11070.102153246480004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTCG2100.020.5703932
AAACGCT2200.020.36260829
CGTCTTA659.437108E-619.69131515
CTAGCGG2150.019.34786229
TCTAGCG2200.018.90813428
TAAACGC2300.018.78166228
TAGCGGC2400.017.33245830
GTAAACG2500.017.27912727
AACGCTT2550.016.94032130
CCGTGCT1151.8373612E-816.6978939
CTAAGAC1252.8539944E-916.6399273
ACGCTTC2650.016.30106431
GTTCAAA4150.016.1919541
TATTCTG1102.0168409E-716.0014085
TACACCG805.6161538E-516.0014085
TAACGAA1501.1459633E-1015.9999313
ACGAACG1601.8189894E-1115.99919315
GCGCAAG3850.015.79140951
ATCGTTT2250.015.64365629
GATAACG1652.910383E-1115.51723211
Produced by FastQC (version 0.11.5)