##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083666
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118848427467505	32.0	32.0	32.0	32.0	32.0
2	31.122881958094098	32.0	32.0	32.0	32.0	32.0
3	31.26215180692206	32.0	32.0	32.0	32.0	32.0
4	31.39157821690447	32.0	32.0	32.0	32.0	32.0
5	31.268942644689414	32.0	32.0	32.0	32.0	32.0
6	34.7988411558543	36.0	36.0	36.0	32.0	36.0
7	34.831438838166	36.0	36.0	36.0	32.0	36.0
8	34.757239776831604	36.0	36.0	36.0	32.0	36.0
9	34.89621894568991	36.0	36.0	36.0	32.0	36.0
10	34.704873088202454	36.0	36.0	36.0	32.0	36.0
11	34.91386183565785	36.0	36.0	36.0	36.0	36.0
12	34.7930746189324	36.0	36.0	36.0	32.0	36.0
13	34.87339826108783	36.0	36.0	36.0	32.0	36.0
14	34.80821858395483	36.0	36.0	36.0	32.0	36.0
15	34.7683308325628	36.0	36.0	36.0	32.0	36.0
16	34.7779887899039	36.0	36.0	36.0	32.0	36.0
17	34.74410104220304	36.0	36.0	36.0	32.0	36.0
18	34.75491064590012	36.0	36.0	36.0	32.0	36.0
19	34.75106536515864	36.0	36.0	36.0	32.0	36.0
20	34.73915210037041	36.0	36.0	36.0	32.0	36.0
21	34.7295854995912	36.0	36.0	36.0	32.0	36.0
22	34.71654088990519	36.0	36.0	36.0	32.0	36.0
23	34.65382322597553	36.0	36.0	36.0	32.0	36.0
24	34.62839288120141	36.0	36.0	36.0	32.0	36.0
25	34.610647561148916	36.0	36.0	36.0	32.0	36.0
26	34.54858784902359	36.0	36.0	36.0	32.0	36.0
27	34.52970287893133	36.0	36.0	36.0	32.0	36.0
28	34.50513719171774	36.0	36.0	36.0	32.0	36.0
29	34.46208149005321	36.0	36.0	36.0	32.0	36.0
30	34.44343829187222	36.0	36.0	36.0	32.0	36.0
31	34.43536200268348	36.0	36.0	36.0	32.0	36.0
32	34.415719419083004	36.0	36.0	36.0	32.0	36.0
33	34.38088765357592	36.0	36.0	36.0	32.0	36.0
34	34.37072400536697	36.0	36.0	36.0	32.0	36.0
35	34.337158312616616	36.0	36.0	36.0	32.0	36.0
36	34.31434685595008	36.0	36.0	36.0	32.0	36.0
37	34.312537257789764	36.0	36.0	36.0	32.0	36.0
38	33.83869568667837	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	15.0
22	69.0
23	228.0
24	661.0
25	1633.0
26	3632.0
27	7332.0
28	13165.0
29	21129.0
30	32974.0
31	48513.0
32	71161.0
33	114877.0
34	268731.0
35	499538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63405423264553	18.703451789204784	12.06668057152666	26.59581340662302
2	16.00705383393038	20.52542019404503	36.2923631451019	27.175162826922687
3	18.198042570312257	24.004813291180124	28.647203105015752	29.14994103349187
4	12.057877665145595	16.02635500823594	36.01472779872009	35.901039527898384
5	14.202069641383968	36.89051792711038	33.27012197485203	15.637290456653618
6	34.509713813242676	36.08105102785645	16.193133843270463	13.216101315630413
7	30.427781381815898	30.52882774225519	20.460088533222105	18.583302342706805
8	28.38093357314171	32.87835569463375	19.01022753803605	19.73048319418849
9	27.25742492937977	13.95645862498881	18.438962987708713	40.34715345792271
10	15.575535153521258	26.735205936173767	31.45596270420362	26.233296206101354
11	37.92900423951702	21.10825441531763	21.741413455344798	19.221327889820543
12	24.358031447161885	24.033667417517872	28.465688929820175	23.142612205500072
13	29.276564094708498	19.18190358248236	25.318411091281227	26.223121231527912
14	23.36910081150465	19.620897951951985	25.06741006915415	31.94259116738921
15	25.135696792185048	27.23182235116724	22.126282452342327	25.506198404305387
16	25.919356996857886	25.97398642569429	23.33350251230777	24.77315406514006
17	24.181435977505984	25.987712081028658	24.904260168723575	24.926591772741784
18	25.18691183445822	24.85138409805235	25.84643238783906	24.11527167965037
19	25.446401381975626	25.124438710820492	25.540710883242625	23.888449023961257
20	25.744648258780845	24.107335655081915	24.985373722161626	25.162642363975618
21	26.858115746102346	24.244023752728012	24.611111367442888	24.28674913372675
22	25.660144048206778	24.136979601629655	25.400654261233868	24.802222088929696
23	24.306334804884173	24.044445510785632	25.80080172451978	25.848417959810423
24	24.52674532558925	25.024131051449434	25.385312448669605	25.06381117429171
25	24.738803284406817	24.574730590421773	25.453876009766844	25.232590115404562
26	24.591824964357066	25.51074363387209	25.53888886325571	24.35854253851513
27	25.291648902890746	24.7669484878182	24.915795088154468	25.025607521136585
28	24.448123314748273	24.788910974414627	25.891280154586376	24.87168555625073
29	24.346339185690056	25.080606016983094	25.779991251917107	24.793063545409748
30	24.330928533330383	25.299769486170092	25.89109559587548	24.478206384624045
31	24.995247613194472	25.073408227258216	24.821485586887473	25.109858572659842
32	24.72966762821755	24.945693599319345	24.884789224724223	25.43984954773888
33	24.404936576399	24.56218059808096	25.429791097995142	25.6030917275249
34	25.01222701459675	24.80026133513463	25.559074474976605	24.628437175292017
35	25.651630668490107	24.551937589626323	25.452491819435142	24.343939922448428
36	24.43769574758274	25.342771665808467	25.173992724695616	25.045539861913173
37	25.55446050720425	25.111335042346994	24.900568994505687	24.43363545594307
38	24.563380229701774	24.672915824617547	25.608720768207178	25.1549831774735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	34.5
2	48.0
3	48.0
4	168.0
5	288.0
6	288.0
7	458.0
8	628.0
9	741.0
10	854.0
11	854.0
12	1153.5
13	1453.0
14	2038.5
15	2624.0
16	2624.0
17	3872.0
18	5120.0
19	5120.0
20	6138.5
21	7157.0
22	8084.0
23	9011.0
24	9011.0
25	10628.5
26	12246.0
27	12246.0
28	15372.5
29	18499.0
30	22050.5
31	25602.0
32	25602.0
33	31954.5
34	38307.0
35	38307.0
36	42626.0
37	46945.0
38	53255.0
39	59565.0
40	59565.0
41	63871.0
42	68177.0
43	76721.5
44	85266.0
45	85266.0
46	86531.0
47	87796.0
48	87796.0
49	92979.0
50	98162.0
51	99227.0
52	100292.0
53	100292.0
54	95303.5
55	90315.0
56	90315.0
57	87475.0
58	84635.0
59	75943.5
60	67252.0
61	67252.0
62	63607.0
63	59962.0
64	50628.5
65	41295.0
66	41295.0
67	35049.5
68	28804.0
69	28804.0
70	23169.5
71	17535.0
72	13802.0
73	10069.0
74	10069.0
75	7717.0
76	5365.0
77	5365.0
78	4957.0
79	4549.0
80	3591.0
81	2633.0
82	2633.0
83	2329.5
84	2026.0
85	2026.0
86	1341.5
87	657.0
88	515.5
89	374.0
90	374.0
91	226.5
92	79.0
93	54.5
94	30.0
95	30.0
96	23.5
97	17.0
98	17.0
99	12.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010335287810081705
2	0.0
3	0.0
4	9.227935544715808E-5
5	0.0
6	2.7683806634147426E-4
7	4.6139677723579036E-4
8	0.002122425175284636
9	0.004890805838699378
10	4.6139677723579036E-4
11	0.00479852648325222
12	6.459554881301066E-4
13	3.691174217886323E-4
14	0.0
15	0.0
16	9.227935544715808E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	9.227935544715808E-5
22	9.227935544715808E-5
23	1.8455871089431616E-4
24	0.0
25	0.0
26	9.227935544715808E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1083666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.81261138292202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7806916289401	46.12330121004654
2	13.10713103063236	15.155149452379737
3	3.5664323603459303	6.185543042164696
4	1.3205138685269646	3.053694204276335
5	0.636870267622974	1.840956664171227
6	0.3542223874524205	1.228711273735057
7	0.22581537590770492	0.9138483600148418
8	0.1491288987555044	0.6897224855772078
9	0.11513818894963045	0.5990795435770588
>10	0.5804506171119108	6.326145829885689
>50	0.07347503468089817	2.9569473027555246
>100	0.07938526610955064	9.671448781526145
>500	0.008339461166237653	3.3590276638438845
>1k	0.0024056137979531693	1.8964241860460629
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2690	0.2482314661528552	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2003	0.18483554896065763	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1634	0.1507844668006563	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1608	0.14838520355903018	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1284	0.11848669239415095	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1241	0.11451868010992318	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1241	0.11451868010992318	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1177	0.10861280136130506	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1145	0.105659861986996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1113	0.10270692261268695	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1110	0.10243008454634546	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1107	0.102153246480004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.227935544715808E-5
2	0.0	0.0	0.0	9.227935544715808E-5	9.227935544715808E-5
3	0.0	0.0	0.0	3.691174217886323E-4	9.227935544715808E-5
4	0.0	0.0	0.0	3.691174217886323E-4	1.8455871089431616E-4
5	0.0	0.0	0.0	3.691174217886323E-4	2.768380663414742E-4
6	0.0	0.0	0.0	3.691174217886323E-4	2.768380663414742E-4
7	0.0	0.0	0.0	4.6139677723579036E-4	2.768380663414742E-4
8	0.0	0.0	0.0	5.536761326829484E-4	2.768380663414742E-4
9	0.0	0.0	0.0	6.459554881301065E-4	2.768380663414742E-4
10	0.0	0.0	0.0	6.459554881301065E-4	2.768380663414742E-4
11	0.0	0.0	0.0	6.459554881301065E-4	2.768380663414742E-4
12	0.0	0.0	0.0	6.459554881301065E-4	4.6139677723579036E-4
13	0.0	0.0	0.0	6.459554881301065E-4	5.536761326829484E-4
14	0.0	0.0	0.0	7.382348435772646E-4	6.459554881301065E-4
15	0.0	0.0	0.0	7.382348435772646E-4	7.382348435772646E-4
16	0.0	0.0	0.0	7.382348435772646E-4	7.382348435772646E-4
17	0.0	0.0	0.0	7.382348435772646E-4	7.382348435772646E-4
18	0.0	0.0	0.0	8.305141990244227E-4	0.0010150729099187388
19	0.0	0.0	0.0	9.227935544715807E-4	0.0010150729099187388
20	0.0	0.0	0.0	9.227935544715807E-4	0.001291910976260213
21	0.0	0.0	0.0	9.227935544715807E-4	0.001291910976260213
22	0.0	0.0	0.0	0.0014764696871545292	0.001291910976260213
23	0.0	0.0	0.0	0.0023992632416261097	0.001291910976260213
24	0.0	0.0	0.0	0.0032297774406505326	0.0013841903317073712
25	0.0	0.0	0.0	0.003506615506992007	0.0013841903317073712
26	0.0	0.0	0.0	0.0039680122842277975	0.0013841903317073712
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTCG	210	0.0	20.57039	32
AAACGCT	220	0.0	20.362608	29
CGTCTTA	65	9.437108E-6	19.691315	15
CTAGCGG	215	0.0	19.347862	29
TCTAGCG	220	0.0	18.908134	28
TAAACGC	230	0.0	18.781662	28
TAGCGGC	240	0.0	17.332458	30
GTAAACG	250	0.0	17.279127	27
AACGCTT	255	0.0	16.940321	30
CCGTGCT	115	1.8373612E-8	16.697893	9
CTAAGAC	125	2.8539944E-9	16.639927	3
ACGCTTC	265	0.0	16.301064	31
GTTCAAA	415	0.0	16.191954	1
TATTCTG	110	2.0168409E-7	16.001408	5
TACACCG	80	5.6161538E-5	16.001408	5
TAACGAA	150	1.1459633E-10	15.99993	13
ACGAACG	160	1.8189894E-11	15.999193	15
GCGCAAG	385	0.0	15.7914095	1
ATCGTTT	225	0.0	15.643656	29
GATAACG	165	2.910383E-11	15.517232	11
>>END_MODULE