FastQCFastQC Report
Thu 2 Feb 2017
SRR4063079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561100
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49240.3154186150791109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32420.20767407597207097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18610.11921081288834795No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16280.1042854397540196No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15890.10178720133239383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATC3000.016.53305225
CCGACTT3950.016.20225723
TATTTCG600.00244218716.0002416
CTTTCGT3950.015.79669427
CGACTTT4000.015.59973524
AACCGCG3150.015.2373487
TCGTTCT4000.015.19925530
TTTCGTT4000.015.19925528
ATCGTTT3200.014.99926529
AGGCCCG5150.014.91284610
CGAGCCG4300.014.883944515
CGCGGTC2800.014.85736610
GTCTTAC1308.229472E-814.7817641
TCTTATA1851.0913936E-1114.7062222
TTCGTTC4150.014.64988329
TACCGCA4600.014.26017128
AGAACCG3600.014.2201585
TTATACC1351.3218596E-714.2201584
TCGTTTA3500.014.17073330
TGGATAC5250.014.01880824
Produced by FastQC (version 0.11.5)