##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063079_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561100
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231387483184935	32.0	32.0	32.0	32.0	32.0
2	30.865977836141184	32.0	32.0	32.0	32.0	32.0
3	30.92715841393889	32.0	32.0	32.0	32.0	32.0
4	30.986912433540454	32.0	32.0	32.0	32.0	32.0
5	30.91193261162001	32.0	32.0	32.0	32.0	32.0
6	34.56618281980655	36.0	36.0	36.0	32.0	36.0
7	34.48852667990519	36.0	36.0	36.0	32.0	36.0
8	34.47544872205496	36.0	36.0	36.0	32.0	36.0
9	34.5947030939722	36.0	36.0	36.0	32.0	36.0
10	34.33792005637051	36.0	36.0	36.0	32.0	36.0
11	34.59986483889565	36.0	36.0	36.0	32.0	36.0
12	34.42049964768432	36.0	36.0	36.0	32.0	36.0
13	34.509341489975014	36.0	36.0	36.0	32.0	36.0
14	34.41111972327205	36.0	36.0	36.0	32.0	36.0
15	34.366253282941514	36.0	36.0	36.0	32.0	36.0
16	34.38311382999167	36.0	36.0	36.0	32.0	36.0
17	34.31213247069374	36.0	36.0	36.0	32.0	36.0
18	34.32919800140926	36.0	36.0	36.0	32.0	36.0
19	34.316305169431814	36.0	36.0	36.0	32.0	36.0
20	34.30123630773173	36.0	36.0	36.0	32.0	36.0
21	34.27601691115239	36.0	36.0	36.0	32.0	36.0
22	34.25578117993722	36.0	36.0	36.0	32.0	36.0
23	34.20986483889565	36.0	36.0	36.0	32.0	36.0
24	34.19321440010249	36.0	36.0	36.0	32.0	36.0
25	34.16580295945167	36.0	36.0	36.0	32.0	36.0
26	34.1368669527897	36.0	36.0	36.0	32.0	36.0
27	34.139613093331626	36.0	36.0	36.0	32.0	36.0
28	34.11175132919095	36.0	36.0	36.0	32.0	36.0
29	34.0724117609378	36.0	36.0	36.0	32.0	36.0
30	34.0560252386138	36.0	36.0	36.0	32.0	36.0
31	34.06548395362245	36.0	36.0	36.0	32.0	36.0
32	34.02488950099289	36.0	36.0	36.0	32.0	36.0
33	33.985619755300746	36.0	36.0	36.0	32.0	36.0
34	33.986577413362376	36.0	36.0	36.0	32.0	36.0
35	33.94152969060278	36.0	36.0	36.0	32.0	36.0
36	33.90080199859074	36.0	36.0	36.0	32.0	36.0
37	33.89951892896035	36.0	36.0	36.0	32.0	36.0
38	33.30610531035808	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	5.0
9	5.0
10	12.0
11	14.0
12	9.0
13	12.0
14	228.0
15	454.0
16	580.0
17	727.0
18	911.0
19	1117.0
20	1493.0
21	1997.0
22	2962.0
23	4516.0
24	6573.0
25	9837.0
26	14520.0
27	20806.0
28	29361.0
29	39940.0
30	53494.0
31	72699.0
32	98904.0
33	148422.0
34	333350.0
35	718149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.425534302800976	17.39891936957881	11.373939811771496	25.801606515848714
2	16.42405353319195	20.19402641666394	37.88674379271206	25.49517625743205
3	18.740838783082882	24.71190215051559	28.84719858955698	27.700060476844545
4	12.425298629319132	15.733779016545926	36.69577316278989	35.14514919134505
5	14.288203385835043	36.79475649096914	33.95533383042127	14.961706292774545
6	33.1338367403414	36.15402255851017	17.323662350041253	13.388478351107173
7	29.188795841903687	30.961841625675245	21.701762668479066	18.147599863942006
8	27.693481426154626	32.986503686839924	20.048197000434953	19.2718178865705
9	27.817728291241167	14.133385604234968	18.84282729207263	39.20605881245124
10	15.638550219045527	26.97645428741822	32.28325574870395	25.1017397448323
11	36.89620779580173	21.456038834876825	22.695252169824435	18.952501199497014
12	24.52980546501855	24.107270975544758	29.266663079351414	22.096260480085274
13	29.52013822909666	19.883566203062745	25.278447446868352	25.317848120972243
14	23.625351786044895	19.925520335843963	25.72629844525463	30.722829432856514
15	24.94251157617272	27.146325257937054	22.964550994426393	24.94661217146383
16	25.21219599228413	25.911006131565678	24.270006707501064	24.606791168649128
17	23.694819257038148	25.927613116518668	25.70919668370943	24.668370942733763
18	24.524394157881215	25.022661624628682	26.660512527474218	23.79243169001588
19	25.216322854212457	25.220807225218568	25.994681535986757	23.56818838458222
20	25.572449154779083	24.36547572984705	25.63311492800824	24.428960187365632
21	26.645876668073875	24.444285003206264	24.85914541062276	24.0506929180971
22	25.496864806135598	24.665724528311557	25.325883350800904	24.511527314751937
23	24.158769267827367	24.47927777638974	25.81999376023811	25.541959195544784
24	24.426913124507895	25.251443248046467	25.80734440437469	24.514299223070953
25	24.62384350095749	24.674392783945702	25.85701536275357	24.844748352343238
26	24.26775856855097	25.439255162657677	26.10070065555117	24.192285613240188
27	24.977546013055985	25.196320236775847	25.236871945008556	24.589261805159612
28	24.172601404093637	25.02096403155143	26.04274035926809	24.763694205086846
29	24.237669913859484	25.376600479967813	25.971371060221482	24.414358545951217
30	24.23698694723032	25.31331515750957	26.2552464754447	24.194451419815405
31	24.616914199030372	25.246058956776647	25.18007595092402	24.956950893268964
32	24.365003558632228	25.369428306212775	25.17833199764509	25.087236137509905
33	24.100593603737153	25.001889845698905	25.69447024742244	25.2030463031415
34	24.68629057625668	25.10194315015705	25.954581884133642	24.257184389452625
35	25.343654060038233	24.905501540164728	25.762906887944688	23.98793751185235
36	24.215248360536005	25.663729854261323	25.45359144480407	24.667430340398596
37	25.076971567606215	25.48889597851587	25.311121446591407	24.123011007286514
38	24.295233739095227	25.43628692631841	25.63513203597149	24.633347298614876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	485.0
1	425.0
2	365.0
3	365.0
4	865.5
5	1366.0
6	1366.0
7	1608.5
8	1851.0
9	1748.0
10	1645.0
11	1645.0
12	2172.5
13	2700.0
14	3433.0
15	4166.0
16	4166.0
17	6179.0
18	8192.0
19	8192.0
20	10060.5
21	11929.0
22	12656.5
23	13384.0
24	13384.0
25	15715.0
26	18046.0
27	18046.0
28	22798.5
29	27551.0
30	33327.0
31	39103.0
32	39103.0
33	48025.0
34	56947.0
35	56947.0
36	63486.0
37	70025.0
38	79471.5
39	88918.0
40	88918.0
41	95298.0
42	101678.0
43	112908.0
44	124138.0
45	124138.0
46	129392.5
47	134647.0
48	134647.0
49	140469.0
50	146291.0
51	145327.5
52	144364.0
53	144364.0
54	137428.5
55	130493.0
56	130493.0
57	125632.5
58	120772.0
59	107261.5
60	93751.0
61	93751.0
62	87359.0
63	80967.0
64	66430.0
65	51893.0
66	51893.0
67	42981.0
68	34069.0
69	34069.0
70	27181.5
71	20294.0
72	15814.0
73	11334.0
74	11334.0
75	8502.5
76	5671.0
77	5671.0
78	5371.5
79	5072.0
80	3979.0
81	2886.0
82	2886.0
83	2879.0
84	2872.0
85	2872.0
86	1939.5
87	1007.0
88	849.0
89	691.0
90	691.0
91	510.0
92	329.0
93	294.0
94	259.0
95	259.0
96	240.0
97	221.0
98	221.0
99	474.5
100	728.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11690474665300109
2	0.05105374415476266
3	0.011017872013323937
4	0.0032669271667414004
5	5.124591634104157E-4
6	2.5622958170520784E-4
7	6.405739542630196E-5
8	4.4840176798411377E-4
9	1.2811479085260392E-4
10	5.765165588367177E-4
11	0.0018576644673627572
12	0.0028185253987572864
13	0.013644225225802319
14	0.00877586317340337
15	0.02248414579463199
16	0.010313240663634617
17	0.02049836653641663
18	0.005829222983793478
19	0.007879059637435142
20	0.005637050797514573
21	0.005957337774646083
22	0.00717442828774582
23	0.00916020754596118
24	0.013516110434949716
25	0.015822176670296586
26	0.01767984113765934
27	0.008327461405419257
28	0.005957337774646083
29	0.010377298059060918
30	0.003330984562167702
31	0.005893280379219781
32	0.0068541413106143105
33	0.008071231823714048
34	0.011594388572160656
35	0.014605086157196847
36	0.01434885657549164
37	0.008583690987124463
38	0.005508936006661969
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1561100.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.401584865870205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7973661249838	46.602926498211566
2	12.662431086732173	14.790120874400442
3	3.6243452899526614	6.350025271031619
4	1.4140254767785791	3.3032531553834703
5	0.6888521900808657	2.0115029819524115
6	0.4162995943222488	1.458753365246289
7	0.27158338619882116	1.1102630124075596
8	0.1811689410117709	0.8464442626847016
9	0.1315192407417373	0.6912838889706047
>10	0.6775259514926437	7.062145110195964
>50	0.06035930593152755	2.460905626327456
>100	0.06383014537946324	7.782234692717717
>500	0.00793726990052966	3.065347463135771
>1k	0.0027559964932394655	2.4647937973344924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4924	0.3154186150791109	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3242	0.20767407597207097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1861	0.11921081288834795	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1628	0.1042854397540196	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1589	0.10178720133239383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2811479085260395E-4
2	0.0	0.0	0.0	0.0	1.2811479085260395E-4
3	0.0	0.0	0.0	0.0	1.2811479085260395E-4
4	0.0	0.0	0.0	0.0	1.2811479085260395E-4
5	0.0	0.0	0.0	0.0	1.921721862789059E-4
6	0.0	0.0	0.0	0.0	2.562295817052079E-4
7	0.0	0.0	0.0	0.0	2.562295817052079E-4
8	0.0	0.0	0.0	0.0	3.2028697713150985E-4
9	0.0	0.0	0.0	0.0	3.2028697713150985E-4
10	0.0	0.0	0.0	0.0	3.2028697713150985E-4
11	0.0	0.0	0.0	0.0	3.2028697713150985E-4
12	0.0	0.0	0.0	0.0	5.765165588367177E-4
13	0.0	0.0	0.0	0.0	6.405739542630197E-4
14	0.0	0.0	0.0	0.0	6.405739542630197E-4
15	0.0	0.0	0.0	0.0	7.686887451156236E-4
16	0.0	0.0	0.0	6.405739542630197E-5	7.686887451156236E-4
17	0.0	0.0	0.0	6.405739542630197E-5	7.686887451156236E-4
18	0.0	0.0	0.0	6.405739542630197E-5	7.686887451156236E-4
19	0.0	0.0	0.0	6.405739542630197E-5	7.686887451156236E-4
20	0.0	0.0	0.0	1.921721862789059E-4	0.0010249183268208316
21	0.0	0.0	0.0	4.4840176798411377E-4	0.0010249183268208316
22	0.0	0.0	0.0	7.686887451156236E-4	0.0012170905130997373
23	0.0	0.0	0.0	0.0012170905130997373	0.0012170905130997373
24	0.0	0.0	0.0	0.002177951444494267	0.0012170905130997373
25	0.0	0.0	0.0	0.0029466401896098907	0.0012170905130997373
26	0.0	0.0	0.0	0.003523156748446608	0.0014092626993786432
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATC	300	0.0	16.533052	25
CCGACTT	395	0.0	16.202257	23
TATTTCG	60	0.002442187	16.00024	16
CTTTCGT	395	0.0	15.796694	27
CGACTTT	400	0.0	15.599735	24
AACCGCG	315	0.0	15.237348	7
TCGTTCT	400	0.0	15.199255	30
TTTCGTT	400	0.0	15.199255	28
ATCGTTT	320	0.0	14.999265	29
AGGCCCG	515	0.0	14.912846	10
CGAGCCG	430	0.0	14.8839445	15
CGCGGTC	280	0.0	14.857366	10
GTCTTAC	130	8.229472E-8	14.781764	1
TCTTATA	185	1.0913936E-11	14.706222	2
TTCGTTC	415	0.0	14.649883	29
TACCGCA	460	0.0	14.260171	28
AGAACCG	360	0.0	14.220158	5
TTATACC	135	1.3218596E-7	14.220158	4
TCGTTTA	350	0.0	14.170733	30
TGGATAC	525	0.0	14.018808	24
>>END_MODULE