FastQCFastQC Report
Thu 2 Feb 2017
SRR4063079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561100
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54470.3489206328870668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39410.25245019537505603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22140.14182307347383255No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17540.11235667157773366No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16460.10543847287169303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.10524630068541414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3350.018.1507071
GTATAGA1550.017.5497911
CGCAATC652.1012679E-417.23049412
ATACCGT3250.016.2469336
ACCGTCG3250.016.2464128
TAGCGTA2150.015.6286587
TACCGTC3300.015.0310267
CCGTGTA756.2453677E-414.9335739
AATAGCG2400.014.6673715
TCTAGAT2850.014.59672452
CGTCGTA3650.014.46598410
CTAGCGG2550.014.43114229
CCGTCGT3600.014.222459
GTCCTAT5550.014.1272571
CAAATCG2053.6379788E-1214.04855513
CGTTTAT2650.013.8865731
GTATTAT1402.0548032E-713.71538451
ATAGCGT2450.013.7149466
GACGTGA1402.0556035E-713.7149457
TTAGTAT1053.6838755E-513.7145054
Produced by FastQC (version 0.11.5)