##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561100
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18612901159439	32.0	32.0	32.0	32.0	32.0
2	31.284523733265004	32.0	32.0	32.0	32.0	32.0
3	31.365894561527128	32.0	32.0	32.0	32.0	32.0
4	31.47112100441996	32.0	32.0	32.0	32.0	32.0
5	31.386803535968227	32.0	32.0	32.0	32.0	32.0
6	34.912768560630326	36.0	36.0	36.0	36.0	36.0
7	34.949477291653324	36.0	36.0	36.0	36.0	36.0
8	34.89097431298443	36.0	36.0	36.0	32.0	36.0
9	34.989729037217344	36.0	36.0	36.0	36.0	36.0
10	34.84392607776568	36.0	36.0	36.0	32.0	36.0
11	35.02705400038435	36.0	36.0	36.0	36.0	36.0
12	34.92582730126193	36.0	36.0	36.0	32.0	36.0
13	34.97581961437448	36.0	36.0	36.0	36.0	36.0
14	34.92649734161809	36.0	36.0	36.0	32.0	36.0
15	34.893207994362946	36.0	36.0	36.0	32.0	36.0
16	34.90763115751714	36.0	36.0	36.0	32.0	36.0
17	34.874590993530205	36.0	36.0	36.0	32.0	36.0
18	34.866990583562874	36.0	36.0	36.0	32.0	36.0
19	34.86502594324515	36.0	36.0	36.0	32.0	36.0
20	34.85852603933124	36.0	36.0	36.0	32.0	36.0
21	34.84549099993594	36.0	36.0	36.0	32.0	36.0
22	34.82451668695151	36.0	36.0	36.0	32.0	36.0
23	34.76965793350843	36.0	36.0	36.0	32.0	36.0
24	34.74715457049516	36.0	36.0	36.0	32.0	36.0
25	34.7414649926334	36.0	36.0	36.0	32.0	36.0
26	34.67104349497149	36.0	36.0	36.0	32.0	36.0
27	34.659759144193195	36.0	36.0	36.0	32.0	36.0
28	34.625414771635384	36.0	36.0	36.0	32.0	36.0
29	34.58793671129332	36.0	36.0	36.0	32.0	36.0
30	34.568920632887064	36.0	36.0	36.0	32.0	36.0
31	34.56061238870028	36.0	36.0	36.0	32.0	36.0
32	34.52921017231439	36.0	36.0	36.0	32.0	36.0
33	34.495277688809175	36.0	36.0	36.0	32.0	36.0
34	34.47398372942156	36.0	36.0	36.0	32.0	36.0
35	34.447673435398116	36.0	36.0	36.0	32.0	36.0
36	34.42057715713279	36.0	36.0	36.0	32.0	36.0
37	34.41199538786753	36.0	36.0	36.0	32.0	36.0
38	33.982504003587216	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	30.0
22	97.0
23	344.0
24	916.0
25	2438.0
26	5069.0
27	9938.0
28	17554.0
29	28140.0
30	43295.0
31	62663.0
32	90468.0
33	145029.0
34	355501.0
35	799616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71419044318269	18.561153752276645	12.070079995694954	26.654575808845713
2	15.48728460700788	21.2104285439754	37.1454102876177	26.156876561399013
3	17.643392479661777	25.487733008775866	29.250528473512265	27.618346038050095
4	11.978876406221776	16.5692352433992	36.66208015127814	34.78980819910089
5	13.75255509099679	37.40377772325778	33.61913626233826	15.224530923407166
6	33.185659589814605	36.549123406010764	16.964907414584214	13.300309589590412
7	29.00339697891157	31.09193366442614	21.676287062974467	18.22838229368782
8	27.610555863749514	33.21371421836105	19.848488148483153	19.32724176940629
9	27.658574052258317	13.930020313211658	18.585467629862663	39.825938004667364
10	15.361372271059151	27.225062472335054	32.357498698025935	25.056066558579865
11	37.06305980810057	21.25147178842633	22.65426712943923	19.031201274033872
12	24.390204843001527	24.21699342385936	29.230323784925737	22.162477948213372
13	29.519912983988135	19.753672429299492	25.348474016906113	25.377940569806263
14	23.501584460690832	19.843072092160714	25.763196312341496	30.89214713480695
15	24.79559285119467	27.23182371404779	22.98609954519249	24.98648388956505
16	25.140926269937864	25.97501761578374	24.204791493177886	24.679264621100504
17	23.60835308436359	25.997757991160082	25.67817564537826	24.715713279098072
18	24.548011017872014	24.9729677791301	26.613093331625137	23.86592787137275
19	25.176798411376595	25.27160335660752	25.95599256934213	23.595605662673755
20	25.495163666645315	24.297162257382617	25.737492793543016	24.470181282429056
21	26.565387589127916	24.455079402395363	24.917574093635317	24.0619589148414
22	25.34491748756324	24.65963059333879	25.417238379653295	24.578213539444675
23	24.0330203484983	24.348631540108308	26.017136656379037	25.601211455014354
24	24.420985202741658	25.23560310037794	25.769457433860737	24.573954263019665
25	24.588142448456153	24.686342561453543	25.87050909039663	24.855005899693676
26	24.388315931074242	25.292998526679906	26.148356927807313	24.17032861443854
27	25.023509064121452	25.040099929536865	25.305361603997184	24.631029402344502
28	24.180705912497597	24.884184229069245	26.160143488565758	24.7749663698674
29	24.178015501889693	25.127474216898342	26.119402985074625	24.57510729613734
30	24.219204407148805	25.197617064890142	26.241944782525145	24.34123374543591
31	24.716546025238614	25.031836525526874	25.209275510857726	25.042341938376783
32	24.390878226891292	25.15553135609506	25.311639228748962	25.141951188264684
33	24.058740631605918	24.787905963743516	25.91326628659279	25.24008711805778
34	24.659342771122926	24.92261866632503	26.10832105566588	24.30971750688617
35	25.330215873422585	24.758311447056563	25.80552174748575	24.105950932035103
36	24.342899237716995	25.41035167510089	25.562872333610915	24.683876753571198
37	25.111331753250916	25.265517904042024	25.382166421113318	24.24098392159375
38	24.281804036771533	25.153177344934562	25.76678352878325	24.79823508951066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	242.5
2	208.0
3	208.0
4	725.0
5	1242.0
6	1242.0
7	1486.5
8	1731.0
9	1694.5
10	1658.0
11	1658.0
12	2253.0
13	2848.0
14	3565.5
15	4283.0
16	4283.0
17	6551.0
18	8819.0
19	8819.0
20	10503.5
21	12188.0
22	12881.0
23	13574.0
24	13574.0
25	15694.0
26	17814.0
27	17814.0
28	22587.5
29	27361.0
30	33041.0
31	38721.0
32	38721.0
33	47907.5
34	57094.0
35	57094.0
36	63782.5
37	70471.0
38	79700.5
39	88930.0
40	88930.0
41	95278.0
42	101626.0
43	113513.5
44	125401.0
45	125401.0
46	129943.5
47	134486.0
48	134486.0
49	140735.0
50	146984.0
51	145830.5
52	144677.0
53	144677.0
54	137617.0
55	130557.0
56	130557.0
57	125411.5
58	120266.0
59	107189.0
60	94112.0
61	94112.0
62	87601.0
63	81090.0
64	66445.0
65	51800.0
66	51800.0
67	43105.0
68	34410.0
69	34410.0
70	27320.5
71	20231.0
72	15800.5
73	11370.0
74	11370.0
75	8484.5
76	5599.0
77	5599.0
78	5182.0
79	4765.0
80	3756.5
81	2748.0
82	2748.0
83	2596.5
84	2445.0
85	2445.0
86	1563.5
87	682.0
88	564.0
89	446.0
90	446.0
91	269.5
92	93.0
93	67.5
94	42.0
95	42.0
96	39.5
97	37.0
98	37.0
99	25.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009032092755108577
2	0.0
3	0.0
4	1.2811479085260392E-4
5	6.405739542630196E-5
6	6.405739542630196E-5
7	4.4840176798411377E-4
8	0.002177951444494267
9	0.0030106975850361923
10	5.765165588367177E-4
11	0.004740247261546345
12	1.2811479085260392E-4
13	5.124591634104157E-4
14	6.405739542630196E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	6.405739542630196E-5
22	1.2811479085260392E-4
23	1.2811479085260392E-4
24	0.0
25	1.2811479085260392E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.405739542630196E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1561100.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.516487230400514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3018350600721	44.25344661289492
2	13.513101214793611	15.274260244979857
3	3.9400390009418267	6.680314916520262
4	1.5738643971776223	3.5579714842188435
5	0.7477762375041114	2.1130843089049023
6	0.42382651416131356	1.4371911465301825
7	0.29413778643109606	1.163654411556792
8	0.1987269967708134	0.8985081420266817
9	0.15458287333749346	0.7862832888315362
>10	0.7087635791958274	7.19013513037264
>50	0.06349060908494371	2.525252699232043
>100	0.0688059500509204	8.257114892255542
>500	0.007632322598434557	2.8896081535731146
>1k	0.003303542617232868	2.6224917792404083
>5k	1.1391526266320236E-4	0.3506827888624369
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5447	0.3489206328870668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3941	0.25245019537505603	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2214	0.14182307347383255	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1754	0.11235667157773366	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1646	0.10543847287169303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.10524630068541414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2811479085260395E-4
2	0.0	0.0	0.0	0.0	1.2811479085260395E-4
3	0.0	0.0	0.0	0.0	1.2811479085260395E-4
4	0.0	0.0	0.0	0.0	1.2811479085260395E-4
5	0.0	0.0	0.0	0.0	1.2811479085260395E-4
6	0.0	0.0	0.0	0.0	1.921721862789059E-4
7	0.0	0.0	0.0	0.0	1.921721862789059E-4
8	0.0	0.0	0.0	0.0	2.562295817052079E-4
9	0.0	0.0	0.0	0.0	2.562295817052079E-4
10	0.0	0.0	0.0	0.0	2.562295817052079E-4
11	0.0	0.0	0.0	0.0	2.562295817052079E-4
12	0.0	0.0	0.0	0.0	8.327461405419255E-4
13	0.0	0.0	0.0	0.0	8.327461405419255E-4
14	0.0	0.0	0.0	0.0	8.968035359682275E-4
15	0.0	0.0	0.0	0.0	0.0010889757222471335
16	0.0	0.0	0.0	6.405739542630197E-5	0.0011530331176734354
17	0.0	0.0	0.0	6.405739542630197E-5	0.0011530331176734354
18	0.0	0.0	0.0	6.405739542630197E-5	0.0012170905130997373
19	0.0	0.0	0.0	6.405739542630197E-5	0.0012170905130997373
20	0.0	0.0	0.0	1.921721862789059E-4	0.0014092626993786432
21	0.0	0.0	0.0	4.4840176798411377E-4	0.0015373774902312472
22	0.0	0.0	0.0	7.686887451156236E-4	0.0015373774902312472
23	0.0	0.0	0.0	0.0012811479085260394	0.0015373774902312472
24	0.0	0.0	0.0	0.002370123630773173	0.0015373774902312472
25	0.0	0.0	0.0	0.0030106975850361923	0.0015373774902312472
26	0.0	0.0	0.0	0.003523156748446608	0.0017295496765101532
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	335	0.0	18.150707	1
GTATAGA	155	0.0	17.549791	1
CGCAATC	65	2.1012679E-4	17.230494	12
ATACCGT	325	0.0	16.246933	6
ACCGTCG	325	0.0	16.246412	8
TAGCGTA	215	0.0	15.628658	7
TACCGTC	330	0.0	15.031026	7
CCGTGTA	75	6.2453677E-4	14.933573	9
AATAGCG	240	0.0	14.667371	5
TCTAGAT	285	0.0	14.5967245	2
CGTCGTA	365	0.0	14.465984	10
CTAGCGG	255	0.0	14.431142	29
CCGTCGT	360	0.0	14.22245	9
GTCCTAT	555	0.0	14.127257	1
CAAATCG	205	3.6379788E-12	14.048555	13
CGTTTAT	265	0.0	13.88657	31
GTATTAT	140	2.0548032E-7	13.7153845	1
ATAGCGT	245	0.0	13.714946	6
GACGTGA	140	2.0556035E-7	13.714945	7
TTAGTAT	105	3.6838755E-5	13.714505	4
>>END_MODULE