FastQCFastQC Report
Thu 2 Feb 2017
SRR4063078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832358
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25630.3079203900244847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18170.21829549304505994No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14120.169638544953013No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13780.16555376412553252No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13530.16255024881120864No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13070.1570237806328527No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12950.15558209328197722No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12670.15221815612993447No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12200.14657154733900557No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11870.14260690712409804No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11460.13768114200860687No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11100.13335607995598048No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10940.1314338301548132No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10640.12782961177762453No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10310.123864971562717No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10200.12254342482441449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9850.11833850338436105No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9730.11689681603348559No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9650.11593569113290195No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9610.11545512868261013No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9560.11485442561974535No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT9480.1138933007191617No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9010.1082466919282328No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8890.10680500457735734No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8820.10596402028934665No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC8670.10416191110075232No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT8340.10019727088584479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC555.8319085E-520.363143
TCGGCAA507.194837E-419.19953213
CATCGTT2300.018.087628
GTGTATT1700.017.88191811
AATCGGT450.00885258717.77627619
CTAGCGG2800.017.71491629
GTCCTAA1650.017.4740661
TCTAGCG2850.017.40412928
CGTTATT1850.017.3062322
GTGCTAT753.201105E-517.0857521
CTAGAAC753.2326374E-517.066253
ATCGTTT2400.016.66726129
TAGCGGC2900.016.55231530
GTTATTC1950.016.4098573
GCGTTAT2000.016.0178951
CCTAATA600.002432744716.0082662
CGTGATT1203.104833E-816.0005728
TATACAC805.6190438E-515.9996093
TCCGTGA1407.421477E-1015.99864826
TAAGAGG4100.015.6074994
Produced by FastQC (version 0.11.5)