##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063078_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832358
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231160149839372	32.0	32.0	32.0	32.0	32.0
2	30.85696659370127	32.0	32.0	32.0	32.0	32.0
3	30.914023773424415	32.0	32.0	32.0	32.0	32.0
4	30.967077867936634	32.0	32.0	32.0	32.0	32.0
5	30.90219833292886	32.0	32.0	32.0	32.0	32.0
6	34.5518719108845	36.0	36.0	36.0	32.0	36.0
7	34.46676069671944	36.0	36.0	36.0	32.0	36.0
8	34.45789792372993	36.0	36.0	36.0	32.0	36.0
9	34.57506385473558	36.0	36.0	36.0	32.0	36.0
10	34.313007143560824	36.0	36.0	36.0	32.0	36.0
11	34.571425996986875	36.0	36.0	36.0	32.0	36.0
12	34.37824349618794	36.0	36.0	36.0	32.0	36.0
13	34.46892923477638	36.0	36.0	36.0	32.0	36.0
14	34.379464124811676	36.0	36.0	36.0	32.0	36.0
15	34.3268413350986	36.0	36.0	36.0	32.0	36.0
16	34.333029778052236	36.0	36.0	36.0	32.0	36.0
17	34.254885518010276	36.0	36.0	36.0	32.0	36.0
18	34.28700991640616	36.0	36.0	36.0	32.0	36.0
19	34.270793336521066	36.0	36.0	36.0	32.0	36.0
20	34.25192044769198	36.0	36.0	36.0	32.0	36.0
21	34.230194219314285	36.0	36.0	36.0	32.0	36.0
22	34.209752294085	36.0	36.0	36.0	32.0	36.0
23	34.149427289699865	36.0	36.0	36.0	32.0	36.0
24	34.135940304532426	36.0	36.0	36.0	32.0	36.0
25	34.10250517205337	36.0	36.0	36.0	32.0	36.0
26	34.0689667186475	36.0	36.0	36.0	32.0	36.0
27	34.06424038694888	36.0	36.0	36.0	32.0	36.0
28	34.04311366022793	36.0	36.0	36.0	32.0	36.0
29	34.017714733323885	36.0	36.0	36.0	32.0	36.0
30	33.98339536593629	36.0	36.0	36.0	32.0	36.0
31	34.00753762203283	36.0	36.0	36.0	32.0	36.0
32	33.95600330626966	36.0	36.0	36.0	32.0	36.0
33	33.912113537684505	36.0	36.0	36.0	32.0	36.0
34	33.923212127473995	36.0	36.0	36.0	32.0	36.0
35	33.87508139526502	36.0	36.0	36.0	32.0	36.0
36	33.82282503442028	36.0	36.0	36.0	32.0	36.0
37	33.8219480079485	36.0	36.0	36.0	32.0	36.0
38	33.203543427227224	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	11.0
11	8.0
12	6.0
13	4.0
14	165.0
15	299.0
16	413.0
17	483.0
18	520.0
19	670.0
20	877.0
21	1220.0
22	1818.0
23	2516.0
24	3661.0
25	5613.0
26	7987.0
27	11521.0
28	15885.0
29	21405.0
30	29096.0
31	39143.0
32	53638.0
33	80318.0
34	179003.0
35	376074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.546414567847776	17.55370330278319	11.232229198239157	25.667652931129876
2	16.9531252817207	19.962473300390293	36.93651352680058	26.147887891088427
3	18.659042410027684	24.11781951707167	28.65957109350969	28.56356697939096
4	12.44841295109758	15.157791791967378	35.926332192360974	36.46746306457407
5	14.69705907441158	36.529998546296845	33.16365453765632	15.609287841635261
6	34.125501437484154	35.56610925360152	16.766003049172408	13.542386259741916
7	30.203470141453558	30.481475518947377	21.129610095655956	18.18544424394311
8	28.480790625142667	32.12299093895146	19.73739538705887	19.658823048847005
9	27.782069472594088	13.77581975041959	18.836148431502348	39.60596234548397
10	16.430749416714523	25.82915442227706	30.963988879731456	26.776107281276957
11	37.90065046003167	20.980159407161068	22.187246946538288	18.931943186268974
12	25.208058755047535	23.713591970560003	28.23555843366329	22.842790840729176
13	29.49863920647413	18.914128821787116	25.403762158524934	26.183469813213815
14	23.995914811811	19.90339731458953	24.66516475924424	31.43552311435523
15	25.330071175446335	27.046429270977413	22.2568973369592	25.366602216617057
16	26.158912945004424	25.38500264939016	23.582335957090695	24.873748448514718
17	24.147407404737102	25.74186160248114	25.16135001688302	24.949380975898734
18	25.46701461478203	23.825554962826264	26.49632107344877	24.211109348942937
19	25.834020189980944	24.55238173049427	25.41337556200093	24.200222517523855
20	25.91804515614337	23.66912888436067	24.990268072549718	25.42255788694624
21	27.62972138752353	23.804672096580212	23.978527050845667	24.587079465050586
22	26.130095386622802	23.979661106552108	24.828636892839935	25.061606613985155
23	24.12463564032383	23.569174871017275	25.741275192783213	26.564914295875674
24	24.940522250686097	24.785399474192666	25.182999216575908	25.09107905854533
25	25.03625839770926	24.099127515407567	25.251105175187842	25.61350891169533
26	24.614007365973528	25.281150678018037	25.849279925018475	24.25556203098996
27	25.615023699814365	24.49377316664364	24.612242200688467	25.27896093285353
28	24.393227067221105	24.27211878223697	25.96258859347385	25.372065557068073
29	24.513044554425704	24.608927706746545	25.75267673800229	25.125351000825457
30	24.352149210228166	24.865407789467866	26.149275106358182	24.633167893945785
31	25.2850500414509	24.64129951580541	24.39535749900878	25.678292943734903
32	24.622314389178978	24.86981951349624	24.44725858037789	26.06060751694689
33	24.241725180347093	24.120492524186453	25.525421610892433	26.112360684574014
34	25.33007146942483	24.395489784245967	25.5414277543605	24.7330109919687
35	26.24548949019674	24.093422075573496	25.477426122565706	24.18366231166406
36	24.516034456448207	25.181588461968257	24.844185384246593	25.45819169733694
37	25.771502585693142	24.93127246861603	24.689644731530077	24.607580214160755
38	24.713748483137294	24.608859679686656	25.28120532013312	25.396186517042928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	440.0
1	346.5
2	253.0
3	253.0
4	540.0
5	827.0
6	827.0
7	935.0
8	1043.0
9	961.5
10	880.0
11	880.0
12	1136.5
13	1393.0
14	1791.5
15	2190.0
16	2190.0
17	3194.0
18	4198.0
19	4198.0
20	4872.0
21	5546.0
22	5771.0
23	5996.0
24	5996.0
25	6769.5
26	7543.0
27	7543.0
28	9597.5
29	11652.0
30	14563.5
31	17475.0
32	17475.0
33	22989.0
34	28503.0
35	28503.0
36	31468.5
37	34434.0
38	39500.0
39	44566.0
40	44566.0
41	47247.0
42	49928.0
43	56780.0
44	63632.0
45	63632.0
46	65036.5
47	66441.0
48	66441.0
49	71239.5
50	76038.0
51	77289.0
52	78540.0
53	78540.0
54	74500.0
55	70460.0
56	70460.0
57	68569.0
58	66678.0
59	59966.0
60	53254.0
61	53254.0
62	51818.5
63	50383.0
64	41505.0
65	32627.0
66	32627.0
67	27346.0
68	22065.0
69	22065.0
70	17767.0
71	13469.0
72	10372.0
73	7275.0
74	7275.0
75	5512.5
76	3750.0
77	3750.0
78	3851.0
79	3952.0
80	3099.0
81	2246.0
82	2246.0
83	2312.5
84	2379.0
85	2379.0
86	1573.5
87	768.0
88	658.5
89	549.0
90	549.0
91	398.5
92	248.0
93	208.0
94	168.0
95	168.0
96	155.0
97	142.0
98	142.0
99	284.5
100	427.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11269189459343215
2	0.050098635442922396
3	0.01225434248244145
4	0.0032437965394697954
5	1.2014061257295539E-4
6	1.2014061257295539E-4
7	0.0
8	4.8056245029182154E-4
9	1.2014061257295539E-4
10	4.8056245029182154E-4
11	0.0028833747017509292
12	0.002522952864032063
13	0.014777295346473512
14	0.009971670843555297
15	0.022706575776288568
16	0.01093279574413894
17	0.019342638624245817
18	0.006007030628647769
19	0.007688999204669145
20	0.005526468178355948
21	0.006607733691512546
22	0.0073285773669502785
23	0.00913068655554461
24	0.013696029833316914
25	0.016459263922494888
26	0.018381513723662174
27	0.008770264717825743
28	0.005406327565782992
29	0.011413358194430762
30	0.003484077764615706
31	0.0057667494035018585
32	0.00624731185379368
33	0.008890405330398699
34	0.013696029833316914
35	0.016218982697348977
36	0.01429673289618169
37	0.010332092681274163
38	0.0057667494035018585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	832358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.18272697171915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.28390079160886	43.76082229968159
2	10.742370220801293	11.42617084964003
3	2.961185843184909	4.7245181463186885
4	1.2256849812053776	2.6074107883512925
5	0.6886196523428842	1.8311335478955888
6	0.40596146242754105	1.2954082570394243
7	0.28105162492817554	1.0462964283459222
8	0.20843809465683158	0.88682450229117
9	0.14791107850743093	0.7079683053917893
>10	0.8308724347153675	8.276240884091138
>50	0.0969097833226435	3.6784741875550067
>100	0.11007251011704823	12.37321161543693
>500	0.01339026411631947	4.818952646788772
>1k	0.003631258065442568	2.56656754117261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2563	0.3079203900244847	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1817	0.21829549304505994	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1412	0.169638544953013	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1378	0.16555376412553252	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1353	0.16255024881120864	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1307	0.1570237806328527	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1295	0.15558209328197722	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1267	0.15221815612993447	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1220	0.14657154733900557	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1187	0.14260690712409804	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1146	0.13768114200860687	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1110	0.13335607995598048	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1094	0.1314338301548132	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1064	0.12782961177762453	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1031	0.123864971562717	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1020	0.12254342482441449	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	985	0.11833850338436105	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	973	0.11689681603348559	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	965	0.11593569113290195	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	961	0.11545512868261013	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	956	0.11485442561974535	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	948	0.1138933007191617	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	901	0.1082466919282328	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	889	0.10680500457735734	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	882	0.10596402028934665	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	867	0.10416191110075232	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	834	0.10019727088584479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.2014061257295539E-4	0.0
8	0.0	0.0	0.0	1.2014061257295539E-4	0.0
9	0.0	0.0	0.0	2.4028122514591077E-4	0.0
10	0.0	0.0	0.0	2.4028122514591077E-4	0.0
11	0.0	0.0	0.0	2.4028122514591077E-4	0.0
12	0.0	0.0	0.0	2.4028122514591077E-4	0.0
13	0.0	0.0	0.0	2.4028122514591077E-4	0.0
14	0.0	0.0	0.0	2.4028122514591077E-4	1.2014061257295539E-4
15	0.0	0.0	0.0	2.4028122514591077E-4	4.8056245029182154E-4
16	0.0	0.0	0.0	2.4028122514591077E-4	4.8056245029182154E-4
17	0.0	0.0	0.0	2.4028122514591077E-4	4.8056245029182154E-4
18	0.0	0.0	0.0	2.4028122514591077E-4	4.8056245029182154E-4
19	0.0	0.0	0.0	2.4028122514591077E-4	4.8056245029182154E-4
20	0.0	0.0	0.0	3.6042183771886616E-4	6.007030628647769E-4
21	0.0	0.0	0.0	6.007030628647769E-4	7.208436754377323E-4
22	0.0	0.0	0.0	0.0012014061257295539	7.208436754377323E-4
23	0.0	0.0	0.0	0.0024028122514591077	8.409842880106877E-4
24	0.0	0.0	0.0	0.004445202665199349	9.611249005836431E-4
25	0.0	0.0	0.0	0.004805624502918215	9.611249005836431E-4
26	0.0	0.0	0.0	0.006367452466366635	0.0010812655131565985
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGAAC	55	5.8319085E-5	20.36314	3
TCGGCAA	50	7.194837E-4	19.199532	13
CATCGTT	230	0.0	18.0876	28
GTGTATT	170	0.0	17.881918	11
AATCGGT	45	0.008852587	17.776276	19
CTAGCGG	280	0.0	17.714916	29
GTCCTAA	165	0.0	17.474066	1
TCTAGCG	285	0.0	17.404129	28
CGTTATT	185	0.0	17.306232	2
GTGCTAT	75	3.201105E-5	17.085752	1
CTAGAAC	75	3.2326374E-5	17.06625	3
ATCGTTT	240	0.0	16.667261	29
TAGCGGC	290	0.0	16.552315	30
GTTATTC	195	0.0	16.409857	3
GCGTTAT	200	0.0	16.017895	1
CCTAATA	60	0.0024327447	16.008266	2
CGTGATT	120	3.104833E-8	16.00057	28
TATACAC	80	5.6190438E-5	15.999609	3
TCCGTGA	140	7.421477E-10	15.998648	26
TAAGAGG	410	0.0	15.607499	4
>>END_MODULE