FastQCFastQC Report
Thu 2 Feb 2017
SRR4063078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832358
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29520.3546550883153643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21820.26214681663418865No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14380.17276220087990984No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14130.16975868556558596No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13590.16327109248664637No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13360.1605078583974684No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13090.1572640618579986No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12980.1559425151196961No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12560.15089660939163196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12100.14537014121327602No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12040.14464929753783828No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11680.1403242354852119No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG11390.13684015772059618No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11390.13684015772059618No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11320.1359991734325855No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11270.13539847036972072No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10920.13119354892966728No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10480.12590736197645724No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10360.12446567462558178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.11617597235804786No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9640.11581555052032899No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9620.11557526929518308No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC9370.1125717539808592No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9370.1125717539808592No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9350.11233147275571329No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9340.11221133214314033No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8780.10548345783905483No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT8780.10548345783905483No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT8610.10344106742531459No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG8470.10175909884929321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT555.8320795E-520.3630626
CGTAAAC400.0044831919.99943732
TACCGTC2800.018.8566117
CTAGCGG2700.018.36985429
TCTAGCG2850.017.96440728
CGCTTCG2950.017.897832
GGATAGA450.00884665517.7783451
GCATACG450.00884665517.7783451
AAAAGCG450.00884971717.7772775
GGACCGT450.00884971717.7772776
ACCGTCG2900.017.6546758
GGTCGTA550.001364474817.45405429
GACCGAC550.001364474817.4540547
GTATAGG652.099015E-417.231321
CTAAGAC652.0999787E-417.2302843
AACCGCG2050.017.1702487
TAGCGGC2900.017.10296630
CGTCGTA2950.016.81308610
ATACCGT2950.016.8130866
TAAACGC3100.016.51566528
Produced by FastQC (version 0.11.5)