##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832358
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172574781524297	32.0	32.0	32.0	32.0	32.0
2	31.27435670708998	32.0	32.0	32.0	32.0	32.0
3	31.373743028840956	32.0	32.0	32.0	32.0	32.0
4	31.469525132214745	32.0	32.0	32.0	32.0	32.0
5	31.384618157091058	32.0	32.0	32.0	32.0	32.0
6	34.91870925731476	36.0	36.0	36.0	36.0	36.0
7	34.946661172235984	36.0	36.0	36.0	36.0	36.0
8	34.886256875046556	36.0	36.0	36.0	32.0	36.0
9	34.99217524190313	36.0	36.0	36.0	36.0	36.0
10	34.83708932935107	36.0	36.0	36.0	32.0	36.0
11	35.01542245043599	36.0	36.0	36.0	36.0	36.0
12	34.90233168900882	36.0	36.0	36.0	32.0	36.0
13	34.96555328356308	36.0	36.0	36.0	36.0	36.0
14	34.910763157199185	36.0	36.0	36.0	32.0	36.0
15	34.87646181090348	36.0	36.0	36.0	32.0	36.0
16	34.87909769594333	36.0	36.0	36.0	32.0	36.0
17	34.829886899627326	36.0	36.0	36.0	32.0	36.0
18	34.85141249318202	36.0	36.0	36.0	32.0	36.0
19	34.84072838850591	36.0	36.0	36.0	32.0	36.0
20	34.82665511714911	36.0	36.0	36.0	32.0	36.0
21	34.81734061545633	36.0	36.0	36.0	32.0	36.0
22	34.80345476345515	36.0	36.0	36.0	32.0	36.0
23	34.73162148979165	36.0	36.0	36.0	32.0	36.0
24	34.714282796585124	36.0	36.0	36.0	32.0	36.0
25	34.68960591476264	36.0	36.0	36.0	32.0	36.0
26	34.62031842067957	36.0	36.0	36.0	32.0	36.0
27	34.60621991979413	36.0	36.0	36.0	32.0	36.0
28	34.56491557719154	36.0	36.0	36.0	32.0	36.0
29	34.531730337186644	36.0	36.0	36.0	32.0	36.0
30	34.51204409641044	36.0	36.0	36.0	32.0	36.0
31	34.50876065346882	36.0	36.0	36.0	32.0	36.0
32	34.475255839434475	36.0	36.0	36.0	32.0	36.0
33	34.43136366803707	36.0	36.0	36.0	32.0	36.0
34	34.423701099767165	36.0	36.0	36.0	32.0	36.0
35	34.379100098755586	36.0	36.0	36.0	32.0	36.0
36	34.35303679426401	36.0	36.0	36.0	32.0	36.0
37	34.33890465400705	36.0	36.0	36.0	32.0	36.0
38	33.88761806818701	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	10.0
22	63.0
23	179.0
24	534.0
25	1277.0
26	2856.0
27	5625.0
28	9925.0
29	15784.0
30	23441.0
31	34324.0
32	48917.0
33	79114.0
34	192098.0
35	418205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.919604365817435	18.58860677364938	11.933306419443362	26.55848244108982
2	15.946503788033514	20.86229723268113	36.26576545188488	26.92543352740047
3	17.75558113215708	24.67988533779936	29.070784446115734	28.493749083927828
4	11.989341099241189	15.912662370428038	36.20301890056658	35.894977629764185
5	14.204945468175953	36.865747671074224	32.989290665795245	15.940016194954577
6	34.22625841284639	35.97370362272003	16.394027569867774	13.4060103945658
7	29.87758799451675	30.74248545989502	21.112917235836235	18.26700930975199
8	28.390800316700165	32.352796296674526	19.52247477872438	19.733928607900925
9	27.719092942841556	13.583510148765255	18.651525018082033	40.045871890311155
10	16.128648577212573	26.190341745309937	30.861361574827743	26.81964810264974
11	37.93775861820384	20.866434060468002	22.14336519653782	19.052442124790343
12	24.899712262195816	23.786845756918623	28.249124472130283	23.064317508755277
13	29.537361417470407	18.863202764201855	25.552648145745334	26.046787672582404
14	23.880229420513768	19.77898932911079	24.73587086325836	31.60491038711708
15	25.377061312560222	27.095552634803777	22.166183300935415	25.36120275170059
16	26.035672150685162	25.385471155440325	23.499503819270075	25.07935287460444
17	24.09323872660562	25.652063174739716	25.13221474413654	25.122483354518128
18	25.59643807111844	23.6477573351851	26.515153335463825	24.240651258232635
19	25.800076409429597	24.49042359177181	25.468608459340814	24.24089153945778
20	25.98437210911651	23.504069162547847	25.055084470864703	25.456474257470944
21	27.514035443926105	23.753149189590523	24.223139830625563	24.50967553585781
22	26.05585577359742	23.875543936623426	24.946717638323896	25.121882651455262
23	24.055058370667254	23.386471845479022	25.891178382248874	26.66729140160485
24	24.905269126986223	24.699948820099042	25.239380170551613	25.155401882363115
25	24.933802363411807	24.008957705597126	25.522132356828088	25.53510757416298
26	24.761460813736395	24.97062562022591	25.88741863477014	24.380494931267556
27	25.709730668774732	24.140574127959365	24.84579952376261	25.30389567950329
28	24.500154981390217	24.089033805165567	26.070633068943895	25.340178144500324
29	24.524783806967676	24.353583434051213	25.907361976457246	25.21427078252387
30	24.35346329343864	24.588938894081632	26.24615850391298	24.811439308566747
31	25.384750311764893	24.428310895071593	24.551815444796592	25.63512334836693
32	24.83426602495561	24.50099596567823	24.63182909277018	26.032908916595982
33	24.375929587989784	23.955317303371867	25.60292566419738	26.065827444440977
34	25.2785460102504	24.125676692000315	25.8720406363608	24.72373666138849
35	26.348518305825134	23.93885803944937	25.49491925349429	24.217704401231202
36	24.73683198815894	24.895537737367814	25.010872725437856	25.356757549035393
37	25.893545806011353	24.660662839787687	24.92905696827567	24.516734385925286
38	24.717369208922122	24.25747094399285	25.539731701984003	25.485428145101025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	209.0
1	181.5
2	154.0
3	154.0
4	475.5
5	797.0
6	797.0
7	904.0
8	1011.0
9	960.0
10	909.0
11	909.0
12	1175.0
13	1441.0
14	1853.0
15	2265.0
16	2265.0
17	3374.0
18	4483.0
19	4483.0
20	5149.5
21	5816.0
22	5914.5
23	6013.0
24	6013.0
25	6740.0
26	7467.0
27	7467.0
28	9562.5
29	11658.0
30	14423.5
31	17189.0
32	17189.0
33	23008.0
34	28827.0
35	28827.0
36	31600.5
37	34374.0
38	39429.5
39	44485.0
40	44485.0
41	47051.0
42	49617.0
43	56830.5
44	64044.0
45	64044.0
46	65251.5
47	66459.0
48	66459.0
49	71685.0
50	76911.0
51	77302.0
52	77693.0
53	77693.0
54	74173.0
55	70653.0
56	70653.0
57	68902.0
58	67151.0
59	59960.0
60	52769.0
61	52769.0
62	52041.0
63	51313.0
64	41828.0
65	32343.0
66	32343.0
67	27319.5
68	22296.0
69	22296.0
70	18028.0
71	13760.0
72	10572.5
73	7385.0
74	7385.0
75	5521.0
76	3657.0
77	3657.0
78	3779.0
79	3901.0
80	3053.5
81	2206.0
82	2206.0
83	2156.5
84	2107.0
85	2107.0
86	1319.5
87	532.0
88	431.5
89	331.0
90	331.0
91	207.5
92	84.0
93	54.0
94	24.0
95	24.0
96	17.5
97	11.0
98	11.0
99	12.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008890405330398699
2	0.0
3	0.0
4	2.4028122514591077E-4
5	0.0
6	0.0
7	6.007030628647769E-4
8	0.0030035153143238846
9	0.004805624502918215
10	0.00156182796344842
11	0.006007030628647769
12	3.6042183771886616E-4
13	2.4028122514591077E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2014061257295539E-4
22	0.0
23	6.007030628647769E-4
24	0.0
25	2.4028122514591077E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	832358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.02604176087585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82048684310114	41.239495367903956
2	11.653093375331926	11.892224584261452
3	3.154877146335701	4.829426790580749
4	1.3520198109473665	2.7595287733972715
5	0.7323009830938599	1.8683210272438868
6	0.45217334804042986	1.3843569684159616
7	0.31768947662152913	1.1347305550756657
8	0.23159288314170087	0.9453814501368056
9	0.15868895938492114	0.7287542521708428
>10	0.8876509858242395	8.540620422804416
>50	0.10149614810936357	3.7049348219098923
>100	0.11805675813772273	12.646592703059465
>500	0.015378134826846144	5.206447656858898
>1k	0.00449514710323195	3.1191846261806764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2952	0.3546550883153643	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2182	0.26214681663418865	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1438	0.17276220087990984	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1413	0.16975868556558596	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1359	0.16327109248664637	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1336	0.1605078583974684	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1309	0.1572640618579986	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1298	0.1559425151196961	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1256	0.15089660939163196	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1210	0.14537014121327602	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1204	0.14464929753783828	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1168	0.1403242354852119	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1139	0.13684015772059618	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1139	0.13684015772059618	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1132	0.1359991734325855	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1127	0.13539847036972072	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1092	0.13119354892966728	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1048	0.12590736197645724	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1036	0.12446567462558178	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	967	0.11617597235804786	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	964	0.11581555052032899	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	962	0.11557526929518308	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	937	0.1125717539808592	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	937	0.1125717539808592	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	935	0.11233147275571329	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	934	0.11221133214314033	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	878	0.10548345783905483	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	878	0.10548345783905483	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	861	0.10344106742531459	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	847	0.10175909884929321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2014061257295539E-4
2	0.0	0.0	0.0	0.0	1.2014061257295539E-4
3	0.0	0.0	0.0	0.0	1.2014061257295539E-4
4	0.0	0.0	0.0	0.0	2.4028122514591077E-4
5	0.0	0.0	0.0	0.0	2.4028122514591077E-4
6	0.0	0.0	0.0	0.0	2.4028122514591077E-4
7	0.0	0.0	0.0	0.0	2.4028122514591077E-4
8	0.0	0.0	0.0	0.0	2.4028122514591077E-4
9	0.0	0.0	0.0	0.0	2.4028122514591077E-4
10	0.0	0.0	0.0	0.0	2.4028122514591077E-4
11	0.0	0.0	0.0	0.0	2.4028122514591077E-4
12	0.0	0.0	0.0	0.0	2.4028122514591077E-4
13	0.0	0.0	0.0	0.0	2.4028122514591077E-4
14	0.0	0.0	0.0	0.0	2.4028122514591077E-4
15	0.0	0.0	0.0	0.0	2.4028122514591077E-4
16	0.0	0.0	0.0	0.0	3.6042183771886616E-4
17	0.0	0.0	0.0	1.2014061257295539E-4	3.6042183771886616E-4
18	0.0	0.0	0.0	1.2014061257295539E-4	3.6042183771886616E-4
19	0.0	0.0	0.0	1.2014061257295539E-4	3.6042183771886616E-4
20	0.0	0.0	0.0	2.4028122514591077E-4	3.6042183771886616E-4
21	0.0	0.0	0.0	3.6042183771886616E-4	3.6042183771886616E-4
22	0.0	0.0	0.0	9.611249005836431E-4	3.6042183771886616E-4
23	0.0	0.0	0.0	0.0020423904137402416	4.8056245029182154E-4
24	0.0	0.0	0.0	0.0038444996023345723	4.8056245029182154E-4
25	0.0	0.0	0.0	0.004084780827480483	4.8056245029182154E-4
26	0.0	0.0	0.0	0.0057667494035018585	4.8056245029182154E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	55	5.8320795E-5	20.363062	6
CGTAAAC	40	0.00448319	19.999437	32
TACCGTC	280	0.0	18.856611	7
CTAGCGG	270	0.0	18.369854	29
TCTAGCG	285	0.0	17.964407	28
CGCTTCG	295	0.0	17.8978	32
GGATAGA	45	0.008846655	17.778345	1
GCATACG	45	0.008846655	17.778345	1
AAAAGCG	45	0.008849717	17.777277	5
GGACCGT	45	0.008849717	17.777277	6
ACCGTCG	290	0.0	17.654675	8
GGTCGTA	55	0.0013644748	17.454054	29
GACCGAC	55	0.0013644748	17.454054	7
GTATAGG	65	2.099015E-4	17.23132	1
CTAAGAC	65	2.0999787E-4	17.230284	3
AACCGCG	205	0.0	17.170248	7
TAGCGGC	290	0.0	17.102966	30
CGTCGTA	295	0.0	16.813086	10
ATACCGT	295	0.0	16.813086	6
TAAACGC	310	0.0	16.515665	28
>>END_MODULE