FastQCFastQC Report
Thu 2 Feb 2017
SRR4063077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945551
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27890.294960292993186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18980.20072952172860056No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16300.17238625944026287No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15880.1679444049025383No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14200.15017698675164004No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13220.1398126594969494No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13060.1381205244349591No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13050.13801476599358467No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12920.13663990625571756No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12860.1360053556074712No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12860.1360053556074712No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12230.12934257380088435No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11560.12225675822879992No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10870.11495942577396671No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10830.11453639200846913No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10760.11379608291884837No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10750.11369032447747399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10640.11252698162235564No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG10580.11189243097410927No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10530.1113636387672373No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10280.10871967773287744No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10120.10702754267088713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG659.421759E-619.694523
GTATATA1159.258656E-1018.1071871
CGCAAGA3300.017.9490052
AACGAAT450.008854217.77601431
CAAGACG3600.017.7750744
GCACCGT450.00885959717.7741366
GTAAGAC550.001363375817.4565073
CGCACTG550.001365794217.451895
AAGACGG3700.017.2946685
AGTACTG1151.843E-816.6931155
TAGCGGC2400.016.66501430
GTCTAGA1354.3473847E-1016.6111531
TCTAGCG2350.016.33880428
GTCCTAT3950.016.2206551
TCTTATA1102.0057996E-716.0085722
TCCTCGA600.002441367816.00010510
ATCGTTT2800.015.99841429
CGTTTAT2600.015.99841331
CCTATTC3750.015.7884423
CTAGCGG2450.015.67191529
Produced by FastQC (version 0.11.5)