##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063077_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945551
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211232392541493	32.0	32.0	32.0	32.0	32.0
2	30.81736257483732	32.0	32.0	32.0	32.0	32.0
3	30.865089244260755	32.0	32.0	32.0	32.0	32.0
4	30.934192867439197	32.0	32.0	32.0	32.0	32.0
5	30.835096150286976	32.0	32.0	32.0	32.0	32.0
6	34.49275290280482	36.0	36.0	36.0	32.0	36.0
7	34.413696352708634	36.0	36.0	36.0	32.0	36.0
8	34.38637788971721	36.0	36.0	36.0	32.0	36.0
9	34.53554805610697	36.0	36.0	36.0	32.0	36.0
10	34.24815266442529	36.0	36.0	36.0	32.0	36.0
11	34.51557980479107	36.0	36.0	36.0	32.0	36.0
12	34.329066332752014	36.0	36.0	36.0	32.0	36.0
13	34.438254520380184	36.0	36.0	36.0	32.0	36.0
14	34.32109531902562	36.0	36.0	36.0	32.0	36.0
15	34.25773226404498	36.0	36.0	36.0	32.0	36.0
16	34.28689409666956	36.0	36.0	36.0	32.0	36.0
17	34.20179239406441	36.0	36.0	36.0	32.0	36.0
18	34.225717068672125	36.0	36.0	36.0	32.0	36.0
19	34.2247451485959	36.0	36.0	36.0	32.0	36.0
20	34.208297595793354	36.0	36.0	36.0	32.0	36.0
21	34.18192990118989	36.0	36.0	36.0	32.0	36.0
22	34.15872755673676	36.0	36.0	36.0	32.0	36.0
23	34.10449039766232	36.0	36.0	36.0	32.0	36.0
24	34.09266871908549	36.0	36.0	36.0	32.0	36.0
25	34.06125000132198	36.0	36.0	36.0	32.0	36.0
26	34.02153664900148	36.0	36.0	36.0	32.0	36.0
27	34.021769317572506	36.0	36.0	36.0	32.0	36.0
28	34.00838664440099	36.0	36.0	36.0	32.0	36.0
29	33.973962271733626	36.0	36.0	36.0	32.0	36.0
30	33.937980077224815	36.0	36.0	36.0	32.0	36.0
31	33.967388327017794	36.0	36.0	36.0	32.0	36.0
32	33.91798327112974	36.0	36.0	36.0	32.0	36.0
33	33.87351396170064	36.0	36.0	36.0	32.0	36.0
34	33.879013400652106	36.0	36.0	36.0	32.0	36.0
35	33.83362293519863	36.0	36.0	36.0	32.0	36.0
36	33.79198795199836	36.0	36.0	36.0	32.0	36.0
37	33.79941007941402	36.0	36.0	36.0	32.0	36.0
38	33.19544900275078	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	7.0
11	14.0
12	6.0
13	8.0
14	94.0
15	213.0
16	312.0
17	354.0
18	500.0
19	664.0
20	839.0
21	1228.0
22	1839.0
23	2876.0
24	4249.0
25	6275.0
26	9173.0
27	13393.0
28	19012.0
29	25818.0
30	35045.0
31	47244.0
32	64482.0
33	95998.0
34	207556.0
35	408349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.971392359225064	17.04779481445481	11.266548288761618	25.714264537558513
2	17.02508827827471	19.176887758287364	37.12068383520825	26.67734012822968
3	19.26729713749602	23.289483843876496	28.03187117833031	29.411347840297175
4	12.545332054323374	14.764844430013865	35.58601958915859	37.10380392650417
5	14.851705043001518	36.178596961761855	33.36350983768143	15.606188157555195
6	35.08797534554	35.153439959219455	16.30354429014255	13.45504040509799
7	30.953380621457754	30.00102585688133	20.432953907298497	18.61263961436242
8	28.85939400349003	32.115171064459844	19.136269895827827	19.889165036222302
9	27.526624715773888	13.882713764475701	18.449050816984823	40.14161070276558
10	16.29138204365929	25.895009142836596	30.72215189270995	27.091456920794162
11	38.397838260878764	20.87146430327804	21.552454430560477	19.178243005282724
12	25.21882046357364	23.442239435487625	28.009653906193815	23.329286194744924
13	29.49391911760039	18.72877931759574	25.251263456953616	26.52603810785025
14	23.928724792746827	19.481904084987022	24.53065273908417	32.058718383181976
15	25.498822628384282	26.692273353319386	22.177217171642376	25.631686846653956
16	26.385831826984813	25.32950161987555	23.302726869837738	24.981939683301903
17	24.315441622926436	25.51425180408609	24.748083786602688	25.422222786384786
18	25.599869698158745	24.22355649991486	25.89252001857224	24.28405378335415
19	25.76977099365822	24.587192059872486	25.20402421637355	24.43901273009574
20	26.00237757297182	23.76790318793614	24.75087308117804	25.478846157914
21	27.646252517218624	23.661008918145974	24.23764236034725	24.455096204288157
22	26.36473152761722	23.534843185306073	24.934768514160545	25.16565677291616
23	24.502753694774064	23.57568879388486	25.561395177147133	26.360162334193944
24	25.026628686631923	24.758913866908397	25.00557959570937	25.208877850750312
25	25.203778334867096	24.05769047694602	25.09895387088926	25.639577317297622
26	24.83395337472114	25.243000866330217	25.572819867839687	24.350225891108956
27	25.70671574304707	24.189895977111792	24.74359177759092	25.359796502250216
28	24.664937768113244	24.17990134278728	25.9423625271815	25.212798361917976
29	24.553862295969743	24.558833484231815	25.79158240326279	25.095721816535654
30	24.53943478900037	24.8419128884226	25.941727488384736	24.67692483419229
31	25.184452670544687	24.6806980433633	24.541406663141196	25.593442622950818
32	25.053147270292737	24.40438039272924	24.392746164406454	26.14972617257157
33	24.55683322968096	24.083099586659728	25.184035842566853	26.176031341092454
34	25.682173478000582	24.235218957642736	25.219634580520694	24.862972983835995
35	26.25343239503958	23.88767130242732	25.501161408244517	24.35773489428858
36	24.50732442069433	25.090570224846864	24.818727330999927	25.58337802345888
37	25.954185555059624	24.60183655467251	24.776036367251706	24.66794152301616
38	24.79349419456919	24.391693654322502	25.18672778462899	25.62808436647932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	63.5
2	92.0
3	92.0
4	312.5
5	533.0
6	533.0
7	674.5
8	816.0
9	799.5
10	783.0
11	783.0
12	1012.0
13	1241.0
14	1749.0
15	2257.0
16	2257.0
17	3222.0
18	4187.0
19	4187.0
20	5086.5
21	5986.0
22	6283.5
23	6581.0
24	6581.0
25	7534.5
26	8488.0
27	8488.0
28	10797.0
29	13106.0
30	15842.0
31	18578.0
32	18578.0
33	24126.5
34	29675.0
35	29675.0
36	33420.5
37	37166.0
38	42927.0
39	48688.0
40	48688.0
41	52199.5
42	55711.0
43	63930.5
44	72150.0
45	72150.0
46	73582.5
47	75015.0
48	75015.0
49	80353.5
50	85692.0
51	88237.5
52	90783.0
53	90783.0
54	87133.5
55	83484.0
56	83484.0
57	81712.0
58	79940.0
59	71944.5
60	63949.0
61	63949.0
62	60692.0
63	57435.0
64	47482.5
65	37530.0
66	37530.0
67	31553.5
68	25577.0
69	25577.0
70	20635.0
71	15693.0
72	12176.5
73	8660.0
74	8660.0
75	6491.0
76	4322.0
77	4322.0
78	4275.0
79	4228.0
80	3272.0
81	2316.0
82	2316.0
83	2337.5
84	2359.0
85	2359.0
86	1555.5
87	752.0
88	660.0
89	568.0
90	568.0
91	433.0
92	298.0
93	248.5
94	199.0
95	199.0
96	185.5
97	172.0
98	172.0
99	339.0
100	506.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11823793745657295
2	0.05584045704568024
3	0.012691012964927329
4	0.002961236358483043
5	3.172753241231832E-4
6	2.1151688274878883E-4
7	0.0
8	1.0575844137439442E-4
9	1.0575844137439442E-4
10	2.1151688274878883E-4
11	0.0016921350619903107
12	0.002855477917108649
13	0.013642838937296878
14	0.0094125012823211
15	0.02379564930923874
16	0.011421911668434596
17	0.020834412950755696
18	0.005710955834217298
19	0.00793188310307958
20	0.005182163627345325
21	0.006662781806586847
22	0.0076146077789563974
23	0.009941293489193074
24	0.015017698675164006
25	0.0170271090612775
26	0.01945955321288857
27	0.009095225958197919
28	0.005816714275591692
29	0.010893119461562623
30	0.002961236358483043
31	0.005393680510094114
32	0.0068742986893356355
33	0.008777950634074735
34	0.012373737640804143
35	0.015546490882035977
36	0.01607528308890795
37	0.008989467516823524
38	0.006028231158340481
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	945551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.25070231184459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32489019615934	40.92530521247436
2	13.117438546522003	13.707907531764729
3	3.940672215414302	6.177086725085094
4	1.6767411558380234	3.504436119508431
5	0.7986233844833244	2.0864316360957993
6	0.46043335976734734	1.4434779849387662
7	0.28915219242094303	1.0575883590302728
8	0.21388765496491036	0.8940624150200046
9	0.15570117698883376	0.7321946263602641
>10	0.8096577285847166	7.855650895202461
>50	0.0978424355649897	3.5659294416315346
>100	0.09987655519408324	11.20277991980093
>500	0.010599144608845585	3.754637438177407
>1k	0.004484253488357747	3.092511694910007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2789	0.294960292993186	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1898	0.20072952172860056	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1630	0.17238625944026287	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1588	0.1679444049025383	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1420	0.15017698675164004	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1322	0.1398126594969494	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1306	0.1381205244349591	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1305	0.13801476599358467	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1292	0.13663990625571756	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1286	0.1360053556074712	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1286	0.1360053556074712	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1223	0.12934257380088435	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1156	0.12225675822879992	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1087	0.11495942577396671	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1083	0.11453639200846913	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1076	0.11379608291884837	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1075	0.11369032447747399	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1064	0.11252698162235564	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1058	0.11189243097410927	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1053	0.1113636387672373	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1028	0.10871967773287744	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1012	0.10702754267088713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.057584413743944E-4	0.0
7	0.0	0.0	0.0	1.057584413743944E-4	0.0
8	0.0	0.0	0.0	1.057584413743944E-4	0.0
9	0.0	0.0	0.0	5.28792206871972E-4	0.0
10	0.0	0.0	0.0	5.28792206871972E-4	0.0
11	0.0	0.0	0.0	5.28792206871972E-4	0.0
12	0.0	0.0	0.0	5.28792206871972E-4	1.057584413743944E-4
13	0.0	0.0	0.0	5.28792206871972E-4	1.057584413743944E-4
14	0.0	0.0	0.0	5.28792206871972E-4	1.057584413743944E-4
15	0.0	0.0	0.0	6.345506482463664E-4	1.057584413743944E-4
16	0.0	0.0	0.0	6.345506482463664E-4	1.057584413743944E-4
17	0.0	0.0	0.0	6.345506482463664E-4	1.057584413743944E-4
18	0.0	0.0	0.0	8.460675309951552E-4	2.115168827487888E-4
19	0.0	0.0	0.0	0.0011633428551183385	2.115168827487888E-4
20	0.0	0.0	0.0	0.0011633428551183385	3.172753241231832E-4
21	0.0	0.0	0.0	0.0012691012964927328	3.172753241231832E-4
22	0.0	0.0	0.0	0.0016921350619903104	3.172753241231832E-4
23	0.0	0.0	0.0	0.002115168827487888	3.172753241231832E-4
24	0.0	0.0	0.0	0.003490028565355015	3.172753241231832E-4
25	0.0	0.0	0.0	0.004230337654975776	3.172753241231832E-4
26	0.0	0.0	0.0	0.005182163627345326	3.172753241231832E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAG	65	9.421759E-6	19.69452	3
GTATATA	115	9.258656E-10	18.107187	1
CGCAAGA	330	0.0	17.949005	2
AACGAAT	45	0.0088542	17.776014	31
CAAGACG	360	0.0	17.775074	4
GCACCGT	45	0.008859597	17.774136	6
GTAAGAC	55	0.0013633758	17.456507	3
CGCACTG	55	0.0013657942	17.45189	5
AAGACGG	370	0.0	17.294668	5
AGTACTG	115	1.843E-8	16.693115	5
TAGCGGC	240	0.0	16.665014	30
GTCTAGA	135	4.3473847E-10	16.611153	1
TCTAGCG	235	0.0	16.338804	28
GTCCTAT	395	0.0	16.220655	1
TCTTATA	110	2.0057996E-7	16.008572	2
TCCTCGA	60	0.0024413678	16.000105	10
ATCGTTT	280	0.0	15.998414	29
CGTTTAT	260	0.0	15.998413	31
CCTATTC	375	0.0	15.788442	3
CTAGCGG	245	0.0	15.671915	29
>>END_MODULE