FastQCFastQC Report
Thu 2 Feb 2017
SRR4063077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945551
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23770.25138781514693553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17030.18010662566059366No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16860.17830873215722898No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16320.17259777632301165No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14250.150705778958512No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14180.14996546986889125No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13960.14763878415865458No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13640.14425451403467396No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12640.13367866989723454No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12580.13304411924898815No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12420.13135198418699784No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12260.12965984912500753No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11940.12627557900102693No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11520.12183372446330236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11420.1207761400495584No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11010.11644004395320823No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10890.1151709426567155No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10850.11474790889121793No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10810.11432487512572034No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10580.11189243097410927No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10430.11030605435349336No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG9880.10448934007790166No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9870.10438358163652728No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC9520.10068203618842347No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9490.10036476086430028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT2450.020.24439629
TGGATCG400.00448080120.001625
TCGTTTA2450.019.59135230
TCTAGCG3200.019.49951728
CATCGTT2550.019.45049928
ATTCGTG507.193289E-419.20053911
TAGCGGC3250.018.7072330
CTAGCGG3350.018.62640629
ACCGTCG2750.018.6196888
CGTCGTA3000.017.60049410
ATACCGT3100.017.5498096
GTATTAC550.00136414617.4550361
GTCCTAA1101.0621079E-817.4550361
CGTTTAT2750.017.45411331
TACCGTC3050.017.3128787
AAGACGG4000.017.2013935
CCGTCGT3100.017.0336389
CAAGACG4150.016.9643334
GTACTAG957.6865945E-716.8425791
GCATCGT3050.016.7864727
Produced by FastQC (version 0.11.5)