##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945551
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13820301601923	32.0	32.0	32.0	32.0	32.0
2	31.186787386402216	32.0	32.0	32.0	32.0	32.0
3	31.312425242001755	32.0	32.0	32.0	32.0	32.0
4	31.42702403149063	32.0	32.0	32.0	32.0	32.0
5	31.326529187743443	32.0	32.0	32.0	32.0	32.0
6	34.84432251671248	36.0	36.0	36.0	32.0	36.0
7	34.88070659329851	36.0	36.0	36.0	32.0	36.0
8	34.81374246338907	36.0	36.0	36.0	32.0	36.0
9	34.93772202662786	36.0	36.0	36.0	32.0	36.0
10	34.754072493181226	36.0	36.0	36.0	32.0	36.0
11	34.95436840530019	36.0	36.0	36.0	36.0	36.0
12	34.84006045152509	36.0	36.0	36.0	32.0	36.0
13	34.90656770496779	36.0	36.0	36.0	32.0	36.0
14	34.83977278856455	36.0	36.0	36.0	32.0	36.0
15	34.81574764343753	36.0	36.0	36.0	32.0	36.0
16	34.82158656698581	36.0	36.0	36.0	32.0	36.0
17	34.7818573508991	36.0	36.0	36.0	32.0	36.0
18	34.793656820203246	36.0	36.0	36.0	32.0	36.0
19	34.79109429316875	36.0	36.0	36.0	32.0	36.0
20	34.76826844876691	36.0	36.0	36.0	32.0	36.0
21	34.75892363288707	36.0	36.0	36.0	32.0	36.0
22	34.740657034892884	36.0	36.0	36.0	32.0	36.0
23	34.687466884388044	36.0	36.0	36.0	32.0	36.0
24	34.6611774510312	36.0	36.0	36.0	32.0	36.0
25	34.63700107133301	36.0	36.0	36.0	32.0	36.0
26	34.570554100201896	36.0	36.0	36.0	32.0	36.0
27	34.56167567904851	36.0	36.0	36.0	32.0	36.0
28	34.518146562163224	36.0	36.0	36.0	32.0	36.0
29	34.48227012609579	36.0	36.0	36.0	32.0	36.0
30	34.46438320090614	36.0	36.0	36.0	32.0	36.0
31	34.45730796117819	36.0	36.0	36.0	32.0	36.0
32	34.43896310193739	36.0	36.0	36.0	32.0	36.0
33	34.39501518162426	36.0	36.0	36.0	32.0	36.0
34	34.388095406805135	36.0	36.0	36.0	32.0	36.0
35	34.36488460167669	36.0	36.0	36.0	32.0	36.0
36	34.328441300363494	36.0	36.0	36.0	32.0	36.0
37	34.32338498928138	36.0	36.0	36.0	32.0	36.0
38	33.87073780261456	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	21.0
22	45.0
23	189.0
24	560.0
25	1533.0
26	3299.0
27	6439.0
28	11351.0
29	18272.0
30	27969.0
31	40843.0
32	59749.0
33	95670.0
34	226806.0
35	452801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12304460676368	18.44949876356744	11.954473051270172	26.472983578398708
2	16.06260906350801	20.316535349796414	36.2700015863783	27.350854000317277
3	18.270299539633506	24.10160848013486	28.35003082858566	29.27806115164597
4	12.027827219953698	15.662223745147	35.69333794441113	36.61661109048817
5	14.364247648720479	36.841030977770586	32.84419950737614	15.950521866132798
6	35.07446980649378	35.66555373533527	15.91474177490162	13.345234683269332
7	30.658021230039335	30.233610809404503	20.429867579295372	18.678500381260793
8	28.627768259407322	32.52067601582165	18.830403790426633	20.021151934344395
9	27.437994502501795	13.66569330559554	18.142378646270153	40.75393354563251
10	16.173157829054656	26.268425939831335	30.678489162829358	26.879927068284644
11	38.63568098554011	20.8178214554654	21.376672109925625	19.169825449068863
12	25.043625587755646	23.64083820353531	27.836509276121944	23.479026932587097
13	29.47501454180107	18.819734546031412	25.245941515520066	26.459309396647452
14	23.74361615608254	19.547226960787942	24.3748882926463	32.33426859048322
15	25.312965667637176	26.935405916761763	21.94952995660731	25.80209845899375
16	26.426496296868173	25.4396642804037	23.076597666334234	25.05724175639389
17	24.35786118358502	25.695070916322866	24.50708634436429	25.439981555727826
18	25.65266178133173	24.33163309012417	25.685975690364664	24.32972943817943
19	25.857092848508433	24.744937079015305	24.9163715124832	24.481598559993063
20	26.122758053240915	23.735578514538084	24.54611120923144	25.59555222298956
21	27.649410396065782	23.770398180952885	23.98974142033737	24.590450002643962
22	26.257282790669144	23.863863503925227	24.755301406269993	25.12355229913564
23	24.500635079440453	23.44231035660689	25.509147576386678	26.547906987565977
24	25.074691899220664	24.80765183475032	24.737216712794975	25.38043955323404
25	25.129051738087107	24.129317191774955	24.980778403280205	25.760852666857737
26	24.904949600814763	25.2950924910449	25.34765443640798	24.452303471732357
27	25.794644169683433	24.29096746969221	24.456268263199473	25.45812009742488
28	24.657157572674556	24.248929988969394	25.73758580975537	25.356326628600677
29	24.65387906099195	24.54600545079007	25.560334662011886	25.239780826206093
30	24.617603915600533	24.789884416599424	25.78285042266361	24.809661245136432
31	25.39027508828186	24.572868094899164	24.378589838094403	25.658266978724576
32	25.172095423726482	24.454207123677094	24.25453518636224	26.119162266234184
33	24.53352595470789	24.077178280177378	25.141425475727907	26.24787028938682
34	25.632038885263725	24.29123336551915	25.110226735522463	24.96650101369466
35	26.25908068417251	23.942759301190524	25.29361187286566	24.504548141771306
36	24.650389032426595	25.008910148685793	24.761646912752457	25.57905390613515
37	25.987387248281692	24.71669957516834	24.559331014403245	24.73658216214673
38	24.785442562061696	24.311750503145785	25.125455951080376	25.777350983712143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	42.0
2	46.0
3	46.0
4	187.5
5	329.0
6	329.0
7	455.0
8	581.0
9	641.5
10	702.0
11	702.0
12	944.0
13	1186.0
14	1580.5
15	1975.0
16	1975.0
17	2954.5
18	3934.0
19	3934.0
20	4709.5
21	5485.0
22	6053.5
23	6622.0
24	6622.0
25	7617.5
26	8613.0
27	8613.0
28	10909.5
29	13206.0
30	16024.5
31	18843.0
32	18843.0
33	24809.0
34	30775.0
35	30775.0
36	34001.0
37	37227.0
38	42944.0
39	48661.0
40	48661.0
41	51954.5
42	55248.0
43	63841.0
44	72434.0
45	72434.0
46	73740.0
47	75046.0
48	75046.0
49	80873.5
50	86701.0
51	88623.0
52	90545.0
53	90545.0
54	87259.0
55	83973.0
56	83973.0
57	81790.0
58	79607.0
59	71824.5
60	64042.0
61	64042.0
62	60978.5
63	57915.0
64	47965.0
65	38015.0
66	38015.0
67	32024.0
68	26033.0
69	26033.0
70	20887.0
71	15741.0
72	12142.5
73	8544.0
74	8544.0
75	6309.0
76	4074.0
77	4074.0
78	4045.5
79	4017.0
80	3189.5
81	2362.0
82	2362.0
83	2162.5
84	1963.0
85	1963.0
86	1266.5
87	570.0
88	461.5
89	353.0
90	353.0
91	216.5
92	80.0
93	59.0
94	38.0
95	38.0
96	26.0
97	14.0
98	14.0
99	13.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0094125012823211
2	1.0575844137439442E-4
3	0.0
4	2.1151688274878883E-4
5	2.1151688274878883E-4
6	0.0
7	4.2303376549757767E-4
8	0.0011633428551183385
9	0.003172753241231832
10	9.518259723695497E-4
11	0.004124579213601381
12	5.287922068719721E-4
13	1.0575844137439442E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0575844137439442E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	2.1151688274878883E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	945551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.187826674749914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.61898212276623	38.45340194773153
2	14.057935067147262	14.110744171097508
3	4.31723211792118	6.500174917466752
4	1.7586773266705602	3.5305677139101843
5	0.8720708364974967	2.1883669995120267
6	0.5433514044347512	1.6361775665551943
7	0.33347135419612345	1.1715341767772427
8	0.2299609652606072	0.9232992853166031
9	0.16977713040973497	0.7668670674906171
>10	0.8697705661892742	8.053567207095796
>50	0.10431630189802958	3.6795528982052677
>100	0.10685727861511098	11.474019800588216
>500	0.01314514139289858	4.539128230415916
>1k	0.004452386600820487	2.972598017837195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2377	0.25138781514693553	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1703	0.18010662566059366	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1686	0.17830873215722898	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1632	0.17259777632301165	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1425	0.150705778958512	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1418	0.14996546986889125	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1396	0.14763878415865458	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1364	0.14425451403467396	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1264	0.13367866989723454	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1258	0.13304411924898815	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1242	0.13135198418699784	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1226	0.12965984912500753	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1194	0.12627557900102693	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1152	0.12183372446330236	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1142	0.1207761400495584	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1101	0.11644004395320823	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1089	0.1151709426567155	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1085	0.11474790889121793	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1081	0.11432487512572034	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1058	0.11189243097410927	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1043	0.11030605435349336	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	988	0.10448934007790166	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	987	0.10438358163652728	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	952	0.10068203618842347	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	949	0.10036476086430028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.057584413743944E-4	0.0
5	0.0	0.0	0.0	1.057584413743944E-4	0.0
6	0.0	0.0	0.0	1.057584413743944E-4	0.0
7	0.0	0.0	0.0	1.057584413743944E-4	0.0
8	0.0	0.0	0.0	1.057584413743944E-4	0.0
9	0.0	0.0	0.0	2.115168827487888E-4	0.0
10	0.0	0.0	0.0	2.115168827487888E-4	0.0
11	0.0	0.0	0.0	2.115168827487888E-4	0.0
12	0.0	0.0	0.0	2.115168827487888E-4	0.0
13	0.0	0.0	0.0	2.115168827487888E-4	0.0
14	0.0	0.0	0.0	2.115168827487888E-4	0.0
15	0.0	0.0	0.0	4.230337654975776E-4	1.057584413743944E-4
16	0.0	0.0	0.0	8.460675309951552E-4	1.057584413743944E-4
17	0.0	0.0	0.0	8.460675309951552E-4	1.057584413743944E-4
18	0.0	0.0	0.0	9.518259723695496E-4	1.057584413743944E-4
19	0.0	0.0	0.0	9.518259723695496E-4	1.057584413743944E-4
20	0.0	0.0	0.0	0.001057584413743944	1.057584413743944E-4
21	0.0	0.0	0.0	0.0012691012964927328	1.057584413743944E-4
22	0.0	0.0	0.0	0.001586376620615916	1.057584413743944E-4
23	0.0	0.0	0.0	0.0022209272688622824	1.057584413743944E-4
24	0.0	0.0	0.0	0.003701545448103804	1.057584413743944E-4
25	0.0	0.0	0.0	0.004441854537724565	1.057584413743944E-4
26	0.0	0.0	0.0	0.005499438951468509	2.115168827487888E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	245	0.0	20.244396	29
TGGATCG	40	0.004480801	20.00162	5
TCGTTTA	245	0.0	19.591352	30
TCTAGCG	320	0.0	19.499517	28
CATCGTT	255	0.0	19.450499	28
ATTCGTG	50	7.193289E-4	19.200539	11
TAGCGGC	325	0.0	18.70723	30
CTAGCGG	335	0.0	18.626406	29
ACCGTCG	275	0.0	18.619688	8
CGTCGTA	300	0.0	17.600494	10
ATACCGT	310	0.0	17.549809	6
GTATTAC	55	0.001364146	17.455036	1
GTCCTAA	110	1.0621079E-8	17.455036	1
CGTTTAT	275	0.0	17.454113	31
TACCGTC	305	0.0	17.312878	7
AAGACGG	400	0.0	17.201393	5
CCGTCGT	310	0.0	17.033638	9
CAAGACG	415	0.0	16.964333	4
GTACTAG	95	7.6865945E-7	16.842579	1
GCATCGT	305	0.0	16.78647	27
>>END_MODULE