FastQCFastQC Report
Thu 2 Feb 2017
SRR4063076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738137
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33650.19359808806785656No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28390.16333580149320795No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26840.15441820754060237No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23750.13664055249960158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21150.12168200780490837No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20650.11880536459439044No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20380.11725197726071075No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19530.11236168380283028No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19230.11063569787651951No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18500.10643579878916334No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18430.10603306873969082No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17780.10229343256601753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG453.5371102E-421.33392710
AAGACGG6450.018.3534055
ACCGTCG3600.018.2201088
CGCAAGA6600.018.1886042
CAAGACG6550.018.0737194
TAAACGC3850.017.86857228
GCGCAAG6650.017.8275781
GTAAACG3700.017.72818427
CGCTTCG3700.017.72818432
GACGGAC6850.017.0491187
AACGCTT3850.017.03747630
GAGCGAA6900.016.92800716
CATCGTT3650.016.65608228
GTATCAA24500.016.6092451
ACGGACC7200.016.4425378
TACCGTC4000.016.3980987
AAACGCT4100.016.38881529
TCTAGCG4600.016.346428
CTAGCGG4600.016.346429
CGTCGTA3850.016.20824410
Produced by FastQC (version 0.11.5)