##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063076_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1738137
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.235079858492167	32.0	32.0	32.0	32.0	32.0
2	30.845067448653356	32.0	32.0	32.0	32.0	32.0
3	30.889419533673124	32.0	32.0	32.0	32.0	32.0
4	30.959115420706194	32.0	32.0	32.0	32.0	32.0
5	30.865822429417243	32.0	32.0	32.0	32.0	32.0
6	34.5014552938002	36.0	36.0	36.0	32.0	36.0
7	34.44483490081622	36.0	36.0	36.0	32.0	36.0
8	34.42106864993956	36.0	36.0	36.0	32.0	36.0
9	34.58011882837774	36.0	36.0	36.0	32.0	36.0
10	34.29069745365296	36.0	36.0	36.0	32.0	36.0
11	34.55080756004849	36.0	36.0	36.0	32.0	36.0
12	34.37170315113251	36.0	36.0	36.0	32.0	36.0
13	34.46361880565226	36.0	36.0	36.0	32.0	36.0
14	34.351491280606766	36.0	36.0	36.0	32.0	36.0
15	34.30688490032719	36.0	36.0	36.0	32.0	36.0
16	34.32494676771739	36.0	36.0	36.0	32.0	36.0
17	34.24104313986757	36.0	36.0	36.0	32.0	36.0
18	34.26876707647326	36.0	36.0	36.0	32.0	36.0
19	34.25887545112957	36.0	36.0	36.0	32.0	36.0
20	34.250772522534184	36.0	36.0	36.0	32.0	36.0
21	34.240346992210625	36.0	36.0	36.0	32.0	36.0
22	34.206464737819864	36.0	36.0	36.0	32.0	36.0
23	34.157787907397406	36.0	36.0	36.0	32.0	36.0
24	34.14473887846585	36.0	36.0	36.0	32.0	36.0
25	34.11094867665783	36.0	36.0	36.0	32.0	36.0
26	34.065077724022906	36.0	36.0	36.0	32.0	36.0
27	34.076792565833415	36.0	36.0	36.0	32.0	36.0
28	34.06082777134369	36.0	36.0	36.0	32.0	36.0
29	34.019060637912894	36.0	36.0	36.0	32.0	36.0
30	33.996806350707686	36.0	36.0	36.0	32.0	36.0
31	34.01975160761206	36.0	36.0	36.0	32.0	36.0
32	33.9699925840138	36.0	36.0	36.0	32.0	36.0
33	33.93920099508842	36.0	36.0	36.0	32.0	36.0
34	33.958804743239455	36.0	36.0	36.0	32.0	36.0
35	33.91936308818005	36.0	36.0	36.0	32.0	36.0
36	33.87678646734981	36.0	36.0	36.0	32.0	36.0
37	33.8807832754265	36.0	36.0	36.0	32.0	36.0
38	33.28399717628702	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	4.0
9	5.0
10	18.0
11	17.0
12	9.0
13	9.0
14	99.0
15	269.0
16	459.0
17	546.0
18	648.0
19	921.0
20	1335.0
21	1981.0
22	3003.0
23	4868.0
24	7387.0
25	11524.0
26	16976.0
27	24774.0
28	34408.0
29	47101.0
30	63252.0
31	84733.0
32	115075.0
33	171392.0
34	377500.0
35	769820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79408943892583	16.99385672606413	11.18110417644704	26.030949658562996
2	17.173260327971523	18.848116305093644	37.09872762522277	26.879895741712062
3	19.59057227588096	22.420535139918684	27.985463232865616	30.00342935133474
4	12.634378752320794	14.609534253384894	35.71015556722864	37.04593142706567
5	15.034916755555095	35.924905674706324	33.6197324256933	15.420445144045281
6	35.637820164302916	35.02043286725799	16.050171132095336	13.29157583634375
7	31.286198959000355	29.85455116598979	20.027765360267917	18.831484514741934
8	28.572373758004293	32.901681692393545	18.817752412075045	19.70819213752711
9	26.975998398282297	14.202644905398548	18.511058410916533	40.310298285402624
10	15.904237202323419	26.278559461673705	31.25011506632423	26.567088269678642
11	38.45796691205026	20.968531639524883	21.39002758767501	19.183473860749846
12	24.77702535541888	23.541876427572795	28.2133261223527	23.46777209465563
13	29.405892168709364	18.855745439898726	25.263191207779506	26.475171183612407
14	23.311167894425232	19.566211792811355	24.77671058135514	32.34590973140827
15	25.32872040020187	27.076524854651744	21.845138724641384	25.749616020504995
16	26.236252513012424	25.7608659377636	23.232087845823308	24.770793703400663
17	24.29505291203195	25.91003515959926	24.78550342676618	25.00940850160261
18	25.31374277350223	24.664278546967633	25.75699865596347	24.264980023566668
19	25.555719215796387	24.813954746153378	25.217517885315345	24.412808152734897
20	25.68304030176555	24.311666373232345	24.784383310376413	25.220910014625687
21	27.03443831683482	24.09888178316317	24.348358922576356	24.518320977425656
22	25.826660023083765	24.134847855430728	25.032939819955836	25.005552301529672
23	24.446872549239608	23.95411687469541	25.61972771854238	25.979282857522605
24	24.79747190154594	24.992764284855287	25.182590322727282	25.02717349087149
25	25.15068811602309	24.419904698937373	25.113170731145935	25.31623645389361
26	24.724367310768663	25.513401848292688	25.552128528846485	24.21010231209216
27	25.37868282932432	24.637370123555954	24.833285193653847	25.150661853465884
28	24.55107735680792	24.594862058053565	25.705934812001956	25.14812577313656
29	24.509234735886956	24.745029917448672	25.673308019970666	25.07242732669371
30	24.41853975735308	25.069947833271293	25.811915555143862	24.699596854231764
31	25.242501149287726	24.914946954401902	24.608393511515605	25.234158384794764
32	24.92915825915448	24.68900116166071	24.612535233876994	25.76930534530782
33	24.45748235911457	24.450002531633917	25.22974577792507	25.86276933132644
34	25.439039139948775	24.6356180841374	25.296859563288326	24.6284832126255
35	25.847360098695642	24.39120807794378	25.41993983432574	24.341491989034843
36	24.439995880059083	25.237457628191446	24.978004924365134	25.344541567384336
37	25.676877754634692	24.83728236228265	24.87675347242201	24.60908641066065
38	24.632629623918604	24.582055064705514	25.465872816778557	25.31944249459733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	60.0
2	88.0
3	88.0
4	265.0
5	442.0
6	442.0
7	581.5
8	721.0
9	797.0
10	873.0
11	873.0
12	1156.0
13	1439.0
14	2247.0
15	3055.0
16	3055.0
17	4545.5
18	6036.0
19	6036.0
20	7658.0
21	9280.0
22	10679.0
23	12078.0
24	12078.0
25	14612.0
26	17146.0
27	17146.0
28	21719.5
29	26293.0
30	31678.5
31	37064.0
32	37064.0
33	46884.5
34	56705.0
35	56705.0
36	64103.0
37	71501.0
38	82440.0
39	93379.0
40	93379.0
41	100112.0
42	106845.0
43	120881.0
44	134917.0
45	134917.0
46	138214.0
47	141511.0
48	141511.0
49	151189.5
50	160868.0
51	164746.0
52	168624.0
53	168624.0
54	161071.0
55	153518.0
56	153518.0
57	147501.0
58	141484.0
59	127203.5
60	112923.0
61	112923.0
62	106101.0
63	99279.0
64	83379.5
65	67480.0
66	67480.0
67	56734.5
68	45989.0
69	45989.0
70	36586.0
71	27183.0
72	21071.0
73	14959.0
74	14959.0
75	11340.0
76	7721.0
77	7721.0
78	7319.0
79	6917.0
80	5390.0
81	3863.0
82	3863.0
83	3875.0
84	3887.0
85	3887.0
86	2602.0
87	1317.0
88	1121.5
89	926.0
90	926.0
91	668.5
92	411.0
93	356.0
94	301.0
95	301.0
96	284.0
97	267.0
98	267.0
99	541.0
100	815.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12035875192807012
2	0.05252750502405737
3	0.011449039977861354
4	0.002646511753676494
5	1.7259859263107569E-4
6	3.4519718526215137E-4
7	0.0
8	4.027300494725099E-4
9	1.7259859263107569E-4
10	5.177957778932271E-4
11	0.0024163802968350597
12	0.0027040446178868524
13	0.013635288817854979
14	0.00839979817471235
15	0.023818605783088444
16	0.010528514150495617
17	0.01996390388099442
18	0.005120424914721912
19	0.006846410841032668
20	0.005695753556825498
21	0.005868352149456573
22	0.006846410841032668
23	0.008975126816815936
24	0.01323255876838247
25	0.01587907052205896
26	0.018237917954683665
27	0.00799706812523984
28	0.005005359186301195
29	0.01081617847154741
30	0.0032218403957800794
31	0.00598341787787729
32	0.006213549334718724
33	0.007421739483136255
34	0.011161375656809561
35	0.015476340472586454
36	0.014268150324168923
37	0.009032659681026295
38	0.006271082198929083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1738137.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.959656625277745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40531721512653	44.43494285579736
2	12.843888661743383	14.374791984889157
3	3.6818200033822364	6.181001494360467
4	1.511078176656148	3.3823766359851537
5	0.7454129638453455	2.085652675040805
6	0.42196586989544	1.4167839112161267
7	0.262874241282228	1.0297246594448723
8	0.18497808911930602	0.8281048280253112
9	0.1334006783076459	0.6718550536507502
>10	0.663859413036298	6.641473549025208
>50	0.06175944291582086	2.4377787868983063
>100	0.06776242591846794	8.207569387714774
>500	0.011035464990472157	4.163725771268838
>1k	0.004847353780861602	4.144218406682886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3365	0.19359808806785656	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2839	0.16333580149320795	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2684	0.15441820754060237	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2375	0.13664055249960158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2115	0.12168200780490837	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2065	0.11880536459439044	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2038	0.11725197726071075	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1953	0.11236168380283028	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1923	0.11063569787651951	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1850	0.10643579878916334	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1843	0.10603306873969082	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1778	0.10229343256601753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.753286421035856E-5	0.0
2	0.0	0.0	0.0	5.753286421035856E-5	0.0
3	0.0	0.0	0.0	5.753286421035856E-5	0.0
4	0.0	0.0	0.0	5.753286421035856E-5	0.0
5	0.0	0.0	0.0	5.753286421035856E-5	0.0
6	0.0	0.0	0.0	5.753286421035856E-5	1.7259859263107569E-4
7	0.0	0.0	0.0	1.1506572842071712E-4	1.7259859263107569E-4
8	0.0	0.0	0.0	1.1506572842071712E-4	1.7259859263107569E-4
9	0.0	0.0	0.0	1.1506572842071712E-4	1.7259859263107569E-4
10	0.0	0.0	0.0	1.1506572842071712E-4	2.3013145684143424E-4
11	0.0	0.0	0.0	1.1506572842071712E-4	2.3013145684143424E-4
12	0.0	0.0	0.0	1.1506572842071712E-4	4.602629136828685E-4
13	0.0	0.0	0.0	1.1506572842071712E-4	5.177957778932271E-4
14	0.0	0.0	0.0	1.1506572842071712E-4	5.177957778932271E-4
15	0.0	0.0	0.0	1.1506572842071712E-4	6.328615063139442E-4
16	0.0	0.0	0.0	1.1506572842071712E-4	6.328615063139442E-4
17	0.0	0.0	0.0	1.1506572842071712E-4	6.328615063139442E-4
18	0.0	0.0	0.0	2.3013145684143424E-4	6.328615063139442E-4
19	0.0	0.0	0.0	3.4519718526215137E-4	6.328615063139442E-4
20	0.0	0.0	0.0	4.027300494725099E-4	6.328615063139442E-4
21	0.0	0.0	0.0	4.602629136828685E-4	6.903943705243027E-4
22	0.0	0.0	0.0	5.753286421035856E-4	6.903943705243027E-4
23	0.0	0.0	0.0	8.054600989450199E-4	7.479272347346613E-4
24	0.0	0.0	0.0	0.0011506572842071713	7.479272347346613E-4
25	0.0	0.0	0.0	0.0012657230126278884	7.479272347346613E-4
26	0.0	0.0	0.0	0.001323255876838247	7.479272347346613E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCGG	45	3.5371102E-4	21.333927	10
AAGACGG	645	0.0	18.353405	5
ACCGTCG	360	0.0	18.220108	8
CGCAAGA	660	0.0	18.188604	2
CAAGACG	655	0.0	18.073719	4
TAAACGC	385	0.0	17.868572	28
GCGCAAG	665	0.0	17.827578	1
GTAAACG	370	0.0	17.728184	27
CGCTTCG	370	0.0	17.728184	32
GACGGAC	685	0.0	17.049118	7
AACGCTT	385	0.0	17.037476	30
GAGCGAA	690	0.0	16.928007	16
CATCGTT	365	0.0	16.656082	28
GTATCAA	2450	0.0	16.609245	1
ACGGACC	720	0.0	16.442537	8
TACCGTC	400	0.0	16.398098	7
AAACGCT	410	0.0	16.388815	29
TCTAGCG	460	0.0	16.3464	28
CTAGCGG	460	0.0	16.3464	29
CGTCGTA	385	0.0	16.208244	10
>>END_MODULE