FastQCFastQC Report
Thu 2 Feb 2017
SRR4063076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738137
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC29090.16736310198793306No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28610.16460152450583584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25710.14791699388483184No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23400.13462690225223903No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21540.12392578950911236No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20610.118575233137549No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19990.11500819555650676No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19630.11293701244493386No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19490.11213155234598884No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18700.1075864560733705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18150.1044221485418008No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17740.1020633011091761No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17630.10143043960286215No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17440.10033731518286533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5550.021.33298928
ACCGTCG4150.021.2050868
CTAGCGG5900.020.0674729
CGTCGTA4200.019.80977410
ATACCGT4450.019.4165336
CCGTCGT4450.019.056429
TAGCGGC6300.019.04731230
AGCGGCG6450.018.6043531
AAGACGG7300.018.1925325
GTCGTAG4650.017.89269811
TACCGTC4950.017.4552677
ACGGGAC550.001365174717.45426413
GCGAAAG7250.017.21351418
CAAGACG7600.017.0528454
GCGGCGC7300.016.87643832
CGCAAGA7700.016.831382
GACGGAC7550.016.7424167
GCGCAAG7650.016.7341631
GCACCGT1456.91216E-1116.5524086
CGGTCCA7050.016.34063110
Produced by FastQC (version 0.11.5)