FastQCFastQC Report
Thu 2 Feb 2017
SRR4063075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768583
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT60380.3414032589932166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38890.21989355320050008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21420.12111390870544385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA450.00885178717.7778922
TAGGACG450.0088633317.7738694
TTTACCG550.001366536117.4516933
GTAATAC1252.8630893E-916.637283
CGCGGTT4300.016.00055110
AAACGCT3400.015.05849129
TTTCGTT4650.014.79537128
GACCGAT650.00416102214.77015711
CGGTTCG650.004163369514.76890431
TTACCGT650.00416885114.7659814
ATCGTTT3150.014.72983429
CGACTTT4700.014.63838924
TCCGACT5000.014.40008922
TTCGTTC4900.014.36702929
CTTTCGT4800.014.33261127
CCGACTT4950.014.22231123
CTATACT2600.014.1507324
AGGCCCG5000.014.08048410
CGAGCCG4800.014.00127415
GGCATCG3200.013.99929426
Produced by FastQC (version 0.11.5)