##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063075_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768583
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22324708537852	32.0	32.0	32.0	32.0	32.0
2	30.923409871066273	32.0	32.0	32.0	32.0	32.0
3	30.97294839993373	32.0	32.0	32.0	32.0	32.0
4	31.01059435717747	32.0	32.0	32.0	32.0	32.0
5	30.97930150860887	32.0	32.0	32.0	32.0	32.0
6	34.591660103031636	36.0	36.0	36.0	32.0	36.0
7	34.52351628394031	36.0	36.0	36.0	32.0	36.0
8	34.51343420127865	36.0	36.0	36.0	32.0	36.0
9	34.60288603927551	36.0	36.0	36.0	32.0	36.0
10	34.390085735303344	36.0	36.0	36.0	32.0	36.0
11	34.61045763755504	36.0	36.0	36.0	32.0	36.0
12	34.45953964275355	36.0	36.0	36.0	32.0	36.0
13	34.52703265834852	36.0	36.0	36.0	32.0	36.0
14	34.44261140133089	36.0	36.0	36.0	32.0	36.0
15	34.402652858248665	36.0	36.0	36.0	32.0	36.0
16	34.40148186429475	36.0	36.0	36.0	32.0	36.0
17	34.35499775809221	36.0	36.0	36.0	32.0	36.0
18	34.37527783541965	36.0	36.0	36.0	32.0	36.0
19	34.348177608854094	36.0	36.0	36.0	32.0	36.0
20	34.323633666047904	36.0	36.0	36.0	32.0	36.0
21	34.301702549442126	36.0	36.0	36.0	32.0	36.0
22	34.286686007951	36.0	36.0	36.0	32.0	36.0
23	34.245237006122984	36.0	36.0	36.0	32.0	36.0
24	34.227409174463396	36.0	36.0	36.0	32.0	36.0
25	34.207508496915324	36.0	36.0	36.0	32.0	36.0
26	34.160090875011235	36.0	36.0	36.0	32.0	36.0
27	34.16650165697624	36.0	36.0	36.0	32.0	36.0
28	34.15152752231589	36.0	36.0	36.0	32.0	36.0
29	34.12660022175945	36.0	36.0	36.0	32.0	36.0
30	34.10561110222138	36.0	36.0	36.0	32.0	36.0
31	34.10771674272567	36.0	36.0	36.0	32.0	36.0
32	34.07432051535042	36.0	36.0	36.0	32.0	36.0
33	34.02666315349633	36.0	36.0	36.0	32.0	36.0
34	34.02862800332243	36.0	36.0	36.0	32.0	36.0
35	33.9811917224128	36.0	36.0	36.0	32.0	36.0
36	33.94180991222917	36.0	36.0	36.0	32.0	36.0
37	33.946014973569234	36.0	36.0	36.0	32.0	36.0
38	33.30236239972905	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	4.0
9	6.0
10	6.0
11	26.0
12	8.0
13	10.0
14	330.0
15	605.0
16	814.0
17	1028.0
18	1267.0
19	1565.0
20	1956.0
21	2516.0
22	3672.0
23	5161.0
24	7478.0
25	10527.0
26	15274.0
27	22056.0
28	31325.0
29	42564.0
30	57532.0
31	78788.0
32	108674.0
33	167379.0
34	380501.0
35	827510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28039330244523	17.367480083515627	11.462904840192575	25.889221773846565
2	16.391790837899826	20.144366384436175	37.90751906837914	25.556323709284868
3	18.47507994718255	24.805426512210502	28.89860915935997	27.82088438124698
4	12.487362342835043	15.755624464242498	36.60040802386623	35.156605169056235
5	14.321594543874438	36.81595584256531	33.84312055823284	15.019329055327407
6	33.13820481765576	36.120205046087875	17.274725796656977	13.466864339599386
7	29.33461420809767	30.959021996705836	21.677806469925358	18.028557325271137
8	27.733806481817595	32.775710203338505	20.137477679808296	19.353005635035604
9	27.67409144727245	14.204486984488138	18.75773925099317	39.363682317246244
10	15.545896811898386	26.93828255048129	32.19431904537888	25.32150159224144
11	36.731949791722386	21.433963245659022	22.759962523034368	19.074124439584224
12	24.706664653705687	24.07817978568751	29.11493611393359	22.100219446673215
13	29.448752372313546	19.87178762166943	25.32013508897731	25.359324917039714
14	23.532801328203284	19.848292584726117	25.892499677675463	30.72640640939514
15	24.93361694474498	26.905229033558864	23.106167077737314	25.054986943958845
16	25.260198273770712	25.94998877492913	24.119934651426206	24.66987829987395
17	23.641699308110724	25.88154520807012	25.71171167095162	24.765043812867535
18	24.517412068854377	25.10073588368808	26.546492310371693	23.835359737085845
19	25.231516692329485	25.118705847560037	26.134908199114815	23.514869260995663
20	25.513375237207747	24.389592941330807	25.658866424049414	24.438165397412032
21	26.610097802386708	24.422323553094664	24.96154855286848	24.006030091650153
22	25.4249345047553	24.599069580180146	25.42199407503411	24.554001840030445
23	24.119201807739895	24.399173035903722	25.93135247987442	25.550272676481967
24	24.317640598694915	25.384759372350935	25.766988084123117	24.530611944831033
25	24.591817864609812	24.687165456347937	25.80956382718221	24.91145285186004
26	24.374744095245205	25.46600114464359	26.08673276644068	24.072521993670527
27	24.910923360444148	25.24053944168272	25.212378726558892	24.63615847131424
28	24.166810387480737	24.947256817314354	26.099662138282987	24.786270656921925
29	24.14911592199937	25.359930694256267	25.992142031535927	24.498811352208442
30	24.317809526663655	25.372696322541138	26.144975688862676	24.16451846193253
31	24.61103581099917	25.184070605305465	25.255148633373654	24.949744950321715
32	24.270947604130146	25.347081641654324	25.205206790088553	25.17676396412698
33	24.018549797588705	24.920989194364036	25.80346737132223	25.256993636725028
34	24.697429123033615	25.103401709846374	25.832331455495698	24.36683771162431
35	25.323551251027137	24.989551917216122	25.68548754232251	24.001409289434232
36	24.361834541548404	25.577175055745283	25.36471242897884	24.696277973727472
37	24.97551475561125	25.441412973899745	25.32995704620869	24.253115224280315
38	24.148938035032604	25.459055781080448	25.798502318674878	24.593503865212075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	543.0
1	482.0
2	421.0
3	421.0
4	1033.5
5	1646.0
6	1646.0
7	1970.0
8	2294.0
9	2173.5
10	2053.0
11	2053.0
12	2564.0
13	3075.0
14	4078.5
15	5082.0
16	5082.0
17	7426.5
18	9771.0
19	9771.0
20	11956.5
21	14142.0
22	14871.0
23	15600.0
24	15600.0
25	17735.5
26	19871.0
27	19871.0
28	25142.5
29	30414.0
30	36675.5
31	42937.0
32	42937.0
33	52597.0
34	62257.0
35	62257.0
36	70241.0
37	78225.0
38	89134.0
39	100043.0
40	100043.0
41	107757.0
42	115471.0
43	128729.5
44	141988.0
45	141988.0
46	147185.0
47	152382.0
48	152382.0
49	159911.0
50	167440.0
51	166129.0
52	164818.0
53	164818.0
54	156191.0
55	147564.0
56	147564.0
57	141429.0
58	135294.0
59	121609.0
60	107924.0
61	107924.0
62	99857.5
63	91791.0
64	75428.0
65	59065.0
66	59065.0
67	48750.5
68	38436.0
69	38436.0
70	30613.5
71	22791.0
72	17925.5
73	13060.0
74	13060.0
75	9936.5
76	6813.0
77	6813.0
78	6301.5
79	5790.0
80	4475.0
81	3160.0
82	3160.0
83	3263.5
84	3367.0
85	3367.0
86	2164.5
87	962.0
88	845.0
89	728.0
90	728.0
91	513.0
92	298.0
93	256.0
94	214.0
95	214.0
96	185.5
97	157.0
98	157.0
99	426.5
100	696.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.124789167372976
2	0.05637281371583917
3	0.01289167655688198
4	0.0033360040212984067
5	1.1308488207791209E-4
6	2.8271220519478023E-4
7	0.0
8	2.8271220519478023E-4
9	5.654244103895604E-5
10	3.957970872726923E-4
11	0.0016962732311686813
12	0.002487867405714066
13	0.015096831757401265
14	0.010121096945973133
15	0.024652504292984836
16	0.012439337028570328
17	0.021542670035842253
18	0.005484616780778736
19	0.007689771981298023
20	0.005145362134545001
21	0.00672855048363577
22	0.00757668709922011
23	0.01057343647428478
24	0.015266459080518133
25	0.01786741136831011
26	0.019620227040517746
27	0.00915987544831088
28	0.006106583632207253
29	0.01057343647428478
30	0.0029402069340257143
31	0.005880413868051429
32	0.006954720247791594
33	0.008707535919999232
34	0.013400558526232583
35	0.01786741136831011
36	0.015379543962596045
37	0.009668757417661484
38	0.0065589231605189015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1768583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.87832364281313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62443867900002	43.37523507096866
2	13.501892201123022	15.08926204409454
3	4.12978600051335	6.922965561367316
4	1.6822696564330917	3.760096332666094
5	0.8670023017809056	2.42233176089887
6	0.5058822961649059	1.6960712802163234
7	0.33883099007770723	1.3253315406643853
8	0.22511467320097514	1.006322445269616
9	0.1656633825334221	0.8331292894469146
>10	0.8223820499977177	8.075722218423676
>50	0.06233860196647859	2.434478093298812
>100	0.06374232775668726	7.542639197056735
>500	0.007612028179849156	2.844723238728685
>1k	0.002943317562875007	2.3292589341671817
>5k	1.014937090646554E-4	0.3424329927321332
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6038	0.3414032589932166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3889	0.21989355320050008	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2142	0.12111390870544385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.654244103895604E-5
7	0.0	0.0	0.0	5.654244103895604E-5	5.654244103895604E-5
8	0.0	0.0	0.0	5.654244103895604E-5	5.654244103895604E-5
9	0.0	0.0	0.0	1.1308488207791209E-4	5.654244103895604E-5
10	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	5.654244103895604E-5
11	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	5.654244103895604E-5
12	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	1.1308488207791209E-4
13	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	1.6962732311686814E-4
14	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	1.6962732311686814E-4
15	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	2.8271220519478023E-4
16	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	2.8271220519478023E-4
17	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	2.8271220519478023E-4
18	0.0	0.0	5.654244103895604E-5	1.1308488207791209E-4	2.8271220519478023E-4
19	0.0	0.0	5.654244103895604E-5	1.6962732311686814E-4	2.8271220519478023E-4
20	0.0	0.0	5.654244103895604E-5	2.8271220519478023E-4	4.5233952831164834E-4
21	0.0	0.0	5.654244103895604E-5	5.088819693506044E-4	5.088819693506044E-4
22	0.0	0.0	5.654244103895604E-5	5.088819693506044E-4	5.088819693506044E-4
23	0.0	0.0	5.654244103895604E-5	0.0010177639387012089	5.088819693506044E-4
24	0.0	0.0	5.654244103895604E-5	0.0014701034670128572	5.088819693506044E-4
25	0.0	0.0	5.654244103895604E-5	0.0020355278774024177	5.088819693506044E-4
26	0.0	0.0	5.654244103895604E-5	0.0026009522877919783	5.088819693506044E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAA	45	0.008851787	17.77789	22
TAGGACG	45	0.00886333	17.773869	4
TTTACCG	55	0.0013665361	17.451693	3
GTAATAC	125	2.8630893E-9	16.63728	3
CGCGGTT	430	0.0	16.000551	10
AAACGCT	340	0.0	15.058491	29
TTTCGTT	465	0.0	14.795371	28
GACCGAT	65	0.004161022	14.770157	11
CGGTTCG	65	0.0041633695	14.768904	31
TTACCGT	65	0.004168851	14.765981	4
ATCGTTT	315	0.0	14.729834	29
CGACTTT	470	0.0	14.638389	24
TCCGACT	500	0.0	14.400089	22
TTCGTTC	490	0.0	14.367029	29
CTTTCGT	480	0.0	14.332611	27
CCGACTT	495	0.0	14.222311	23
CTATACT	260	0.0	14.150732	4
AGGCCCG	500	0.0	14.080484	10
CGAGCCG	480	0.0	14.001274	15
GGCATCG	320	0.0	13.999294	26
>>END_MODULE