FastQCFastQC Report
Thu 2 Feb 2017
SRR4063075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768583
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT65170.36848708825087656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT45080.2548933242036139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26300.1487066199324544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.1071479257688217No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18110.1023983607215494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA659.431977E-619.6938927
ACCGTAT601.1379205E-418.6681698
GCGAATC652.1017344E-417.23020615
ATCGTTT3700.015.99947729
CGTTTAT3750.015.78615131
TCGTTTA3900.015.58923430
GCATTCG5050.015.52468522
CGTCTTA1551.891749E-1015.48336515
CGTCGTA4050.015.40821210
CGAGCCG4600.014.95603215
CGTATTG5400.014.8143327
ATGCGAA650.004163662414.76874713
AACCGCG2500.014.7211847
CCGTCGT4150.014.6513669
ATACGCT4050.014.618468
TACGCTA4050.014.6180479
ACCGTCG4200.014.4773548
GTCGTAG4200.014.47653711
CATCGTT4100.014.43855228
ATTCGTA5700.014.31532224
Produced by FastQC (version 0.11.5)