##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768583
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163994565140566	32.0	32.0	32.0	32.0	32.0
2	31.219876025043778	32.0	32.0	32.0	32.0	32.0
3	31.328107869407315	32.0	32.0	32.0	32.0	32.0
4	31.441172961630866	32.0	32.0	32.0	32.0	32.0
5	31.342229909481205	32.0	32.0	32.0	32.0	32.0
6	34.86915909516262	36.0	36.0	36.0	32.0	36.0
7	34.8968366200512	36.0	36.0	36.0	36.0	36.0
8	34.83338412729287	36.0	36.0	36.0	32.0	36.0
9	34.953935438709976	36.0	36.0	36.0	32.0	36.0
10	34.79022132407696	36.0	36.0	36.0	32.0	36.0
11	34.984537338649076	36.0	36.0	36.0	36.0	36.0
12	34.87906306913501	36.0	36.0	36.0	32.0	36.0
13	34.93851066079455	36.0	36.0	36.0	36.0	36.0
14	34.880378811737984	36.0	36.0	36.0	32.0	36.0
15	34.8501534844562	36.0	36.0	36.0	32.0	36.0
16	34.85891982451488	36.0	36.0	36.0	32.0	36.0
17	34.83016799324657	36.0	36.0	36.0	32.0	36.0
18	34.82247030532353	36.0	36.0	36.0	32.0	36.0
19	34.815443210751205	36.0	36.0	36.0	32.0	36.0
20	34.805394488129764	36.0	36.0	36.0	32.0	36.0
21	34.78516077560397	36.0	36.0	36.0	32.0	36.0
22	34.7704082873125	36.0	36.0	36.0	32.0	36.0
23	34.71667035134907	36.0	36.0	36.0	32.0	36.0
24	34.697176779376484	36.0	36.0	36.0	32.0	36.0
25	34.68640431350974	36.0	36.0	36.0	32.0	36.0
26	34.61870491800498	36.0	36.0	36.0	32.0	36.0
27	34.60105971842995	36.0	36.0	36.0	32.0	36.0
28	34.57229318612697	36.0	36.0	36.0	32.0	36.0
29	34.54112473092866	36.0	36.0	36.0	32.0	36.0
30	34.52033124823658	36.0	36.0	36.0	32.0	36.0
31	34.50473401587599	36.0	36.0	36.0	32.0	36.0
32	34.474651175545624	36.0	36.0	36.0	32.0	36.0
33	34.43245524807148	36.0	36.0	36.0	32.0	36.0
34	34.42439512310138	36.0	36.0	36.0	32.0	36.0
35	34.387626139118154	36.0	36.0	36.0	32.0	36.0
36	34.362010717054275	36.0	36.0	36.0	32.0	36.0
37	34.351912802509126	36.0	36.0	36.0	32.0	36.0
38	33.9210113407174	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	37.0
22	117.0
23	346.0
24	1123.0
25	2803.0
26	6135.0
27	12116.0
28	20698.0
29	33314.0
30	50701.0
31	73783.0
32	107597.0
33	173108.0
34	414166.0
35	872527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68502873738711	18.64718269559179	12.165041494479842	26.50274707254126
2	15.475326857716034	21.238245533288513	37.021163270256466	26.26526433873898
3	17.53918249807897	25.59093918690839	29.15339568456782	27.716482630444826
4	12.023833823651643	16.67304655319327	36.647613705692535	34.65550591746255
5	13.86392383054683	37.30868158293956	33.543463891714445	15.283930694799169
6	33.18737836300494	36.55035503018802	16.974050397437043	13.288216209369994
7	29.052162929003817	31.153460358458286	21.61379733050469	18.18057938203321
8	27.648289810788178	33.112210192887154	19.852568238220414	19.38693175810425
9	27.704493395358515	13.935253535565433	18.604557799165296	39.75569526991076
10	15.320669582401553	27.176665827945257	32.317669975375516	25.184994614277677
11	36.94630305134467	21.37558460592853	22.62704134975479	19.051070992972004
12	24.441387374489562	24.179538589759684	29.07906804223506	22.300005993515697
13	29.49175581073845	19.81008481950764	25.33808215522179	25.36007721453212
14	23.46239899399689	19.714766001934883	25.878796754237715	30.944038249830513
15	24.90072560914585	26.997941289721773	22.970932096486283	25.130401004646092
16	25.2052209057878	26.059351503068562	24.025688376337655	24.709739214805985
17	23.704117929438425	25.851034415687586	25.62017162892553	24.82467602594846
18	24.58516224570744	24.952744654901693	26.575795424924927	23.886297674465943
19	25.289059094201406	25.10461765153233	26.11141235667198	23.49491089759429
20	25.49826612604554	24.289727991278895	25.66664951545955	24.545356367216016
21	26.585238012578433	24.386415565455508	24.9414361666939	24.08691025527216
22	25.38819234844638	24.62594326980598	25.42845058922911	24.557413792518528
23	24.075063567911222	24.222130623364155	26.03527913055721	25.667526678167413
24	24.398628732719924	25.263614995733874	25.721212971062148	24.616543300484057
25	24.653224620882288	24.488742380893147	25.881950491354644	24.97608250686992
26	24.45935531439576	25.226353527089202	26.09512813365276	24.21916302486228
27	24.974145939597904	25.012199045449428	25.2260993417887	24.787555673163965
28	24.19530211474384	24.8137067923869	26.11508761533951	24.87590347752975
29	24.304711738154218	25.072953884550515	26.023488860856403	24.598845516438868
30	24.3675303901485	25.087711461661684	26.18254274749899	24.362215400690836
31	24.63876447981237	24.97977194171831	25.34266132830633	25.038802250162984
32	24.45681090454901	25.04287330591779	25.24823545177128	25.252080337761928
33	24.088267273857095	24.691744747065872	25.88846551165538	25.331522467421657
34	24.74071050100561	24.807600208754693	25.957164577517705	24.494524712721994
35	25.426004886397756	24.717641185061716	25.757909015296427	24.098444913244105
36	24.46257823353498	25.321175200711533	25.459195299287625	24.757051266465865
37	25.05299440286376	25.224600711416993	25.33434958947361	24.38805529624564
38	24.346834379465097	25.019182066506502	25.85454197974758	24.77944157428082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	319.0
1	280.5
2	242.0
3	242.0
4	827.5
5	1413.0
6	1413.0
7	1714.5
8	2016.0
9	2019.0
10	2022.0
11	2022.0
12	2650.5
13	3279.0
14	4163.5
15	5048.0
16	5048.0
17	7693.5
18	10339.0
19	10339.0
20	12279.5
21	14220.0
22	14976.0
23	15732.0
24	15732.0
25	17764.0
26	19796.0
27	19796.0
28	25167.0
29	30538.0
30	36453.0
31	42368.0
32	42368.0
33	52839.0
34	63310.0
35	63310.0
36	70544.5
37	77779.0
38	88510.0
39	99241.0
40	99241.0
41	107318.5
42	115396.0
43	128381.0
44	141366.0
45	141366.0
46	146696.0
47	152026.0
48	152026.0
49	159706.5
50	167387.0
51	165658.0
52	163929.0
53	163929.0
54	156288.0
55	148647.0
56	148647.0
57	143049.5
58	137452.0
59	123016.0
60	108580.0
61	108580.0
62	100316.0
63	92052.0
64	75751.0
65	59450.0
66	59450.0
67	49062.5
68	38675.0
69	38675.0
70	30997.0
71	23319.0
72	18185.5
73	13052.0
74	13052.0
75	9802.0
76	6552.0
77	6552.0
78	6107.5
79	5663.0
80	4379.0
81	3095.0
82	3095.0
83	2946.0
84	2797.0
85	2797.0
86	1789.0
87	781.0
88	663.0
89	545.0
90	545.0
91	318.0
92	91.0
93	63.5
94	36.0
95	36.0
96	28.0
97	20.0
98	20.0
99	15.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008764078361038187
2	0.0
3	0.0
4	2.2616976415582417E-4
5	0.0
6	5.654244103895604E-5
7	5.088819693506044E-4
8	0.0020355278774024177
9	0.0030532918161036266
10	0.0010177639387012089
11	0.004749565047272308
12	2.8271220519478023E-4
13	2.2616976415582417E-4
14	0.0
15	0.0
16	5.654244103895604E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	5.654244103895604E-5
23	1.1308488207791209E-4
24	0.0
25	1.6962732311686814E-4
26	0.0
27	1.1308488207791209E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.2616976415582417E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1768583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.92304213108693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0285201517709	40.99689095308134
2	14.29553266886178	15.417172207787267
3	4.49597848973724	7.273105125676854
4	1.876961338677446	4.04845861375701
5	0.9428568325948772	2.5420854353798363
6	0.5418152037767088	1.7529794436308952
7	0.3566481678418199	1.3462087926356592
8	0.25208617134094385	1.0874602590305693
9	0.18435720138450926	0.8946991023683553
>10	0.8790940753505295	8.344863732384828
>50	0.06532295649502924	2.4372678500431366
>100	0.06827075817001302	7.621407991634553
>500	0.009179584939880327	3.280653383714338
>1k	0.0032708865877734503	2.5859589140528327
>5k	1.0551247057333711E-4	0.370788194822607
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6517	0.36848708825087656	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4508	0.2548933242036139	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2630	0.1487066199324544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1895	0.1071479257688217	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1811	0.1023983607215494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.654244103895604E-5	0.0
5	0.0	0.0	0.0	5.654244103895604E-5	0.0
6	0.0	0.0	0.0	5.654244103895604E-5	5.654244103895604E-5
7	0.0	0.0	0.0	1.1308488207791209E-4	5.654244103895604E-5
8	0.0	0.0	0.0	1.1308488207791209E-4	5.654244103895604E-5
9	0.0	0.0	0.0	1.1308488207791209E-4	5.654244103895604E-5
10	0.0	0.0	0.0	1.1308488207791209E-4	5.654244103895604E-5
11	0.0	0.0	0.0	1.1308488207791209E-4	5.654244103895604E-5
12	0.0	0.0	0.0	1.1308488207791209E-4	3.3925464623373627E-4
13	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
14	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
15	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
16	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
17	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
18	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
19	0.0	0.0	0.0	1.1308488207791209E-4	3.957970872726923E-4
20	0.0	0.0	0.0	2.2616976415582417E-4	3.957970872726923E-4
21	0.0	0.0	0.0	4.5233952831164834E-4	3.957970872726923E-4
22	0.0	0.0	0.0	5.654244103895605E-4	3.957970872726923E-4
23	0.0	0.0	0.0	0.001130848820779121	3.957970872726923E-4
24	0.0	0.0	0.0	0.0016397307901297254	3.957970872726923E-4
25	0.0	0.0	0.0	0.002205155200519286	3.957970872726923E-4
26	0.0	0.0	0.0	0.0028271220519478025	4.5233952831164834E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	65	9.431977E-6	19.693892	7
ACCGTAT	60	1.1379205E-4	18.668169	8
GCGAATC	65	2.1017344E-4	17.230206	15
ATCGTTT	370	0.0	15.999477	29
CGTTTAT	375	0.0	15.786151	31
TCGTTTA	390	0.0	15.589234	30
GCATTCG	505	0.0	15.524685	22
CGTCTTA	155	1.891749E-10	15.483365	15
CGTCGTA	405	0.0	15.408212	10
CGAGCCG	460	0.0	14.956032	15
CGTATTG	540	0.0	14.81433	27
ATGCGAA	65	0.0041636624	14.768747	13
AACCGCG	250	0.0	14.721184	7
CCGTCGT	415	0.0	14.651366	9
ATACGCT	405	0.0	14.61846	8
TACGCTA	405	0.0	14.618047	9
ACCGTCG	420	0.0	14.477354	8
GTCGTAG	420	0.0	14.476537	11
CATCGTT	410	0.0	14.438552	28
ATTCGTA	570	0.0	14.315322	24
>>END_MODULE