FastQCFastQC Report
Thu 2 Feb 2017
SRR4063074_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063074_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610494
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32100.5258036934023922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22790.37330424213833385No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17510.2868169056534544No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14340.23489174340779762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11540.1890272467870282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.16806061976039077No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9370.15348226190593192No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9100.1490596140175006No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8940.1464387856391709No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8900.14578357854458848No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8600.14086952533522032No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8440.13824869695689065No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8060.13202422955835766No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7970.13055001359554722No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7960.1303862118219016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.12907579763273677No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG7600.12448934797065982No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7350.1203943036295197No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7240.11859248411941804No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG7030.11515264687286034No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA6980.11433363800463232No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT6900.11302322381546746No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC6680.10941958479526416No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG6590.1079453688324537No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT6440.10548834222776965No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA6440.10548834222776965No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG6160.1009018925656927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCAA200.00375398331.99680310
CTATATT350.002066636222.854864
CTATATA350.002066636222.854864
TTAGGAC400.00448157719.9996433
ACCGGTC400.00448370219.9980038
TATGACT400.00448370219.9980034
CACCGGT400.00448370219.9980037
TGCACCG400.00448795619.9947285
GAACTTA507.191516E-419.19965611
CCTATAT450.00884656517.777463
ACCGTCG1453.6379788E-1217.653418
GGCGGGT1550.017.54663732
ATAATTC550.001363815717.4542313
CCGGGCG652.0999732E-417.2290489
ATATACT652.0999732E-417.2290484
CGTTATT957.6268043E-716.8528482
TAGCGTA1551.0913936E-1116.514487
GTTTAAA600.00242313816.0167711
CCTATAG600.00242977916.0102062
ATAGTGG805.614794E-515.9997143
Produced by FastQC (version 0.11.5)