##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063074_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	610494
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148122667872247	32.0	32.0	32.0	32.0	32.0
2	30.77359974053799	32.0	32.0	32.0	32.0	32.0
3	30.83823919645402	32.0	32.0	32.0	32.0	32.0
4	30.886670466867816	32.0	32.0	32.0	32.0	32.0
5	30.83672239203006	32.0	32.0	32.0	32.0	32.0
6	34.45318872912756	36.0	36.0	36.0	32.0	36.0
7	34.353390860516235	36.0	36.0	36.0	32.0	36.0
8	34.327197318892566	36.0	36.0	36.0	32.0	36.0
9	34.433462081527416	36.0	36.0	36.0	32.0	36.0
10	34.19534671921428	36.0	36.0	36.0	32.0	36.0
11	34.4684042758815	36.0	36.0	36.0	32.0	36.0
12	34.27734424908353	36.0	36.0	36.0	32.0	36.0
13	34.381674512771625	36.0	36.0	36.0	32.0	36.0
14	34.25528342620894	36.0	36.0	36.0	32.0	36.0
15	34.23085075365196	36.0	36.0	36.0	32.0	36.0
16	34.23024632510721	36.0	36.0	36.0	32.0	36.0
17	34.13707915229306	36.0	36.0	36.0	32.0	36.0
18	34.18317624743241	36.0	36.0	36.0	32.0	36.0
19	34.15515959206806	36.0	36.0	36.0	32.0	36.0
20	34.12823713255167	36.0	36.0	36.0	32.0	36.0
21	34.093383391155356	36.0	36.0	36.0	32.0	36.0
22	34.079953283734156	36.0	36.0	36.0	32.0	36.0
23	34.0217512375224	36.0	36.0	36.0	32.0	36.0
24	34.02606577624022	36.0	36.0	36.0	32.0	36.0
25	33.99105806117669	36.0	36.0	36.0	32.0	36.0
26	33.93425324409413	36.0	36.0	36.0	32.0	36.0
27	33.93968818694369	36.0	36.0	36.0	32.0	36.0
28	33.91550121704718	36.0	36.0	36.0	32.0	36.0
29	33.88500132679437	36.0	36.0	36.0	32.0	36.0
30	33.83121046234689	36.0	36.0	36.0	32.0	36.0
31	33.85864562141479	36.0	36.0	36.0	32.0	36.0
32	33.81127414847648	36.0	36.0	36.0	32.0	36.0
33	33.76057422349769	36.0	36.0	36.0	32.0	36.0
34	33.7537584316963	36.0	36.0	36.0	32.0	36.0
35	33.67941863474498	36.0	36.0	36.0	27.0	36.0
36	33.627046293657266	36.0	36.0	36.0	27.0	36.0
37	33.63005205620367	36.0	36.0	36.0	27.0	36.0
38	33.0016806061976	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	3.0
12	5.0
13	1.0
14	165.0
15	279.0
16	347.0
17	418.0
18	533.0
19	637.0
20	763.0
21	1073.0
22	1587.0
23	2225.0
24	3153.0
25	4700.0
26	6692.0
27	9230.0
28	12842.0
29	17046.0
30	22561.0
31	30354.0
32	40333.0
33	59437.0
34	129474.0
35	266631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.95064955510149	16.8154581323118	11.268083738656154	24.965808573930552
2	16.621707550509353	20.24962961991793	36.59011052403865	26.53855230553407
3	18.792326720508495	25.052995429451368	28.38965974804646	27.765018101993675
4	12.45749218642502	15.473007947896395	35.62433248809126	36.445167377587325
5	14.415702693228763	36.922721599229476	33.04602502235895	15.61555068518282
6	33.558560772096044	35.49780997028636	17.124001218685194	13.819628038932406
7	29.697916769042777	30.974096387515683	21.116505649523173	18.21148119391837
8	28.91761248695726	31.602164152578325	19.915052261041485	19.565171099422926
9	27.970672882408152	14.152824029104346	18.68539033207588	39.19111275641162
10	16.45981198801624	26.26795065627288	30.693062819721113	26.579174535989765
11	37.329192343099386	21.484171523484722	21.910392116393275	19.276244017022613
12	25.094637869890335	24.30592070239236	28.182510626796724	22.41693080092058
13	29.725080562322553	19.2613987290239	25.423126260450168	25.590394448203384
14	24.38039883164901	19.81626157170402	25.041568853584263	30.761770743062712
15	25.418785718030723	26.55832197347035	23.340422324752268	24.682469983746657
16	25.888774759632316	25.442032440012518	23.954201949808247	24.71499085054692
17	23.8124495169189	25.54521727590427	25.571923133006635	25.07041007417019
18	25.242481477052515	23.877115416432552	27.07503698037051	23.805366126144424
19	25.278853037200612	25.339791988493676	25.94983675896515	23.431518215340564
20	25.545486711747127	23.453307647953032	26.11439822823295	24.886807412066887
21	27.291491250774015	24.04374449180446	24.424608570015103	24.24015568740642
22	25.851389892258574	23.992248252498598	25.295072594820482	24.861289260422346
23	23.994063162447212	23.88315700838431	25.945323429867013	26.177456399301473
24	24.33591202943711	25.592002437801654	25.46372295563791	24.608362577123327
25	24.82604955969691	24.231333196805245	25.520376817530206	25.42224042596764
26	24.264825145609596	25.138728465754095	26.277227560558025	24.31921882807828
27	25.48576015464382	24.880454102402386	25.171721804942376	24.46206393801142
28	24.193683134549136	24.271165561763976	26.50177488815829	25.03337641552859
29	23.7911681061033	25.135315780506662	26.68030745842234	24.393208654967694
30	24.268201191845133	25.063638239948105	26.593740683591	24.074419884615764
31	24.370890434520515	25.13293123613764	25.110489363863014	25.38568896547883
32	24.03813548910239	25.1659827505713	25.11143327517999	25.684448485146326
33	23.904756444175053	24.71286869199833	25.746230147353938	25.636144716472682
34	24.720106158155993	25.06430104356088	26.114087252830064	24.101505545453055
35	25.82481134764787	24.669141619510405	25.883135479093422	23.622911553748306
36	24.14141099721326	25.83098374648381	25.29149348532334	24.736111770979583
37	25.365601138863724	25.5829878135684	25.310885879367596	23.74052516820028
38	24.063119924908715	25.272869342148585	25.699598825142477	24.964411907800223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	915.0
1	610.5
2	306.0
3	306.0
4	713.5
5	1121.0
6	1121.0
7	1279.0
8	1437.0
9	1292.5
10	1148.0
11	1148.0
12	1440.5
13	1733.0
14	2089.5
15	2446.0
16	2446.0
17	3502.5
18	4559.0
19	4559.0
20	5179.0
21	5799.0
22	5515.0
23	5231.0
24	5231.0
25	5730.0
26	6229.0
27	6229.0
28	7949.5
29	9670.0
30	11553.5
31	13437.0
32	13437.0
33	16298.0
34	19159.0
35	19159.0
36	21182.5
37	23206.0
38	27059.5
39	30913.0
40	30913.0
41	33086.0
42	35259.0
43	41079.5
44	46900.0
45	46900.0
46	50377.0
47	53854.0
48	53854.0
49	54997.0
50	56140.0
51	55676.5
52	55213.0
53	55213.0
54	52592.0
55	49971.0
56	49971.0
57	48784.0
58	47597.0
59	42840.0
60	38083.0
61	38083.0
62	37077.0
63	36071.0
64	29741.0
65	23411.0
66	23411.0
67	19548.5
68	15686.0
69	15686.0
70	12725.0
71	9764.0
72	7605.0
73	5446.0
74	5446.0
75	4028.5
76	2611.0
77	2611.0
78	2598.5
79	2586.0
80	2035.5
81	1485.0
82	1485.0
83	1514.0
84	1543.0
85	1543.0
86	1045.5
87	548.0
88	449.0
89	350.0
90	350.0
91	265.0
92	180.0
93	149.5
94	119.0
95	119.0
96	107.5
97	96.0
98	96.0
99	184.0
100	272.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11335082736275869
2	0.05077854983013756
3	0.010483313513318722
4	0.0029484319256208908
5	0.0
6	0.0
7	0.0
8	1.6380177364560503E-4
9	1.6380177364560503E-4
10	1.6380177364560503E-4
11	0.0019656212837472604
12	0.0014742159628104454
13	0.015888772043623687
14	0.009991908192381908
15	0.025880680236005593
16	0.012285133023420377
17	0.02309605008403031
18	0.005733062077596177
19	0.0076986833613434365
20	0.004914053209368151
21	0.006552070945824201
22	0.008353890455925857
23	0.011138520607901142
24	0.017690591553725345
25	0.019492411063827
26	0.021130428800283048
27	0.009664304645090696
28	0.005405458530304966
29	0.011466124155192353
30	0.0019656212837472604
31	0.004586449662076941
32	0.006388269172178596
33	0.009664304645090696
34	0.013759348986230822
35	0.018345798648307765
36	0.016871582685497318
37	0.009991908192381908
38	0.006060665624887386
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	610494.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.0195855640028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6148762547727	44.08801791140194
2	11.461803688345508	12.383237701207662
3	3.1213139432312658	5.058362568854891
4	1.2362164580956212	2.671196029348996
5	0.6076724649962988	1.6413107358878027
6	0.37803657793885853	1.2252827560974586
7	0.24017070903294488	0.9081745516600671
8	0.17051583798196235	0.7368955919907403
9	0.13503550956987792	0.6565106037049865
>10	0.7868745745981063	8.303662147886346
>50	0.11826937528707226	4.504887172903635
>100	0.11705323145330976	12.236560031477195
>500	0.01033716849198049	3.8032618032201295
>1k	0.0018242062044671453	1.7826403943581328
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3210	0.5258036934023922	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2279	0.37330424213833385	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1751	0.2868169056534544	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1434	0.23489174340779762	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1154	0.1890272467870282	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1026	0.16806061976039077	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	937	0.15348226190593192	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	910	0.1490596140175006	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	894	0.1464387856391709	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	890	0.14578357854458848	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	860	0.14086952533522032	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	844	0.13824869695689065	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	806	0.13202422955835766	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	797	0.13055001359554722	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	796	0.1303862118219016	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	788	0.12907579763273677	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	760	0.12448934797065982	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	735	0.1203943036295197	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	724	0.11859248411941804	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	703	0.11515264687286034	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	698	0.11433363800463232	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	690	0.11302322381546746	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	668	0.10941958479526416	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	659	0.1079453688324537	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	644	0.10548834222776965	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	644	0.10548834222776965	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	616	0.1009018925656927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2760354729121006E-4
2	0.0	0.0	0.0	0.0	3.2760354729121006E-4
3	0.0	0.0	0.0	0.0	3.2760354729121006E-4
4	0.0	0.0	0.0	0.0	3.2760354729121006E-4
5	0.0	0.0	0.0	0.0	3.2760354729121006E-4
6	0.0	0.0	0.0	0.0	3.2760354729121006E-4
7	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
8	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
9	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
10	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
11	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
12	0.0	0.0	0.0	1.6380177364560503E-4	6.552070945824201E-4
13	0.0	0.0	0.0	1.6380177364560503E-4	6.552070945824201E-4
14	0.0	0.0	0.0	1.6380177364560503E-4	8.190088682280251E-4
15	0.0	0.0	0.0	1.6380177364560503E-4	8.190088682280251E-4
16	0.0	0.0	0.0	1.6380177364560503E-4	8.190088682280251E-4
17	0.0	0.0	0.0	1.6380177364560503E-4	8.190088682280251E-4
18	0.0	0.0	0.0	3.2760354729121006E-4	8.190088682280251E-4
19	0.0	0.0	0.0	3.2760354729121006E-4	8.190088682280251E-4
20	0.0	0.0	0.0	6.552070945824201E-4	8.190088682280251E-4
21	0.0	0.0	0.0	6.552070945824201E-4	8.190088682280251E-4
22	0.0	0.0	0.0	0.0011466124155192353	8.190088682280251E-4
23	0.0	0.0	0.0	0.0029484319256208908	8.190088682280251E-4
24	0.0	0.0	0.0	0.005405458530304966	8.190088682280251E-4
25	0.0	0.0	0.0	0.0058968638512417815	8.190088682280251E-4
26	0.0	0.0	0.0	0.007862485134989041	8.190088682280251E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCAA	20	0.003753983	31.996803	10
CTATATT	35	0.0020666362	22.85486	4
CTATATA	35	0.0020666362	22.85486	4
TTAGGAC	40	0.004481577	19.999643	3
ACCGGTC	40	0.004483702	19.998003	8
TATGACT	40	0.004483702	19.998003	4
CACCGGT	40	0.004483702	19.998003	7
TGCACCG	40	0.004487956	19.994728	5
GAACTTA	50	7.191516E-4	19.199656	11
CCTATAT	45	0.008846565	17.77746	3
ACCGTCG	145	3.6379788E-12	17.65341	8
GGCGGGT	155	0.0	17.546637	32
ATAATTC	55	0.0013638157	17.454231	3
CCGGGCG	65	2.0999732E-4	17.229048	9
ATATACT	65	2.0999732E-4	17.229048	4
CGTTATT	95	7.6268043E-7	16.852848	2
TAGCGTA	155	1.0913936E-11	16.51448	7
GTTTAAA	60	0.002423138	16.016771	1
CCTATAG	60	0.002429779	16.010206	2
ATAGTGG	80	5.614794E-5	15.999714	3
>>END_MODULE