FastQCFastQC Report
Thu 2 Feb 2017
SRR4063074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610494
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35840.5870655567458485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29070.47617175598777384No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18570.3041798936598885No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17600.28829112161626486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16460.26961771942066587No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.21523553057032505No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9360.1533184601322863No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9320.1526632530377039No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9160.15004242465937423No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8860.14512837145000604No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8750.1433265519399044No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8360.1369382827677258No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8290.13579167035220657No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7890.12923959940638235No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG7880.12907579763273677No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7570.12399794264972301No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7390.12104951072410212No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7330.12006670008222849No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7120.11662686283567078No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG7080.11597165574108836No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG6860.11236801672088505No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG6780.11105760253172021No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG6750.11056619721078341No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT6490.10630735109599766No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA6390.10466933335954161No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.10221230675485754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT401.5916671E-424.0015377
TAAGACT401.5916671E-424.0015374
ATATTAT502.7952212E-522.39961
TAGGACG555.8246846E-520.3649394
AAGGCCG400.004479453420.001285
TACCGTC1700.019.765977
CGTCGTA1800.019.55520610
ACCCGTT902.033994E-819.55520630
GTCGAAG507.1915204E-419.1996561
AATGCCG507.1915204E-419.19965618
CCGTCGT1700.018.8247349
ACCGTCG1700.018.8247348
GTGTATA802.7923033E-617.9996781
ATACCGT2050.017.9523686
CTATATG450.00884239317.7789153
TAATACT1352.3646862E-1117.7789134
GTTTAAG450.00884656617.777461
TCGGCAA450.00884656617.7774613
TAACCCG904.3592627E-717.7774628
GTCGTAG1800.017.7774611
Produced by FastQC (version 0.11.5)