##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	610494
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.113057622187934	32.0	32.0	32.0	32.0	32.0
2	31.288998745278413	32.0	32.0	32.0	32.0	32.0
3	31.37133698283685	32.0	32.0	32.0	32.0	32.0
4	31.467408361097736	32.0	32.0	32.0	32.0	32.0
5	31.402705022489982	32.0	32.0	32.0	32.0	32.0
6	34.90174023004321	36.0	36.0	36.0	36.0	36.0
7	34.93526390103752	36.0	36.0	36.0	36.0	36.0
8	34.852773327829595	36.0	36.0	36.0	32.0	36.0
9	34.97959357503923	36.0	36.0	36.0	36.0	36.0
10	34.83098441589925	36.0	36.0	36.0	32.0	36.0
11	34.99577063820447	36.0	36.0	36.0	36.0	36.0
12	34.894985044898064	36.0	36.0	36.0	32.0	36.0
13	34.943663655990065	36.0	36.0	36.0	36.0	36.0
14	34.87519779064168	36.0	36.0	36.0	32.0	36.0
15	34.852809364219794	36.0	36.0	36.0	32.0	36.0
16	34.85839991875432	36.0	36.0	36.0	32.0	36.0
17	34.827264477619764	36.0	36.0	36.0	32.0	36.0
18	34.795285457350936	36.0	36.0	36.0	32.0	36.0
19	34.80603904379077	36.0	36.0	36.0	32.0	36.0
20	34.79271868355791	36.0	36.0	36.0	32.0	36.0
21	34.76349644713953	36.0	36.0	36.0	32.0	36.0
22	34.75132106130445	36.0	36.0	36.0	32.0	36.0
23	34.68312546888258	36.0	36.0	36.0	32.0	36.0
24	34.66109904438045	36.0	36.0	36.0	32.0	36.0
25	34.63964920212156	36.0	36.0	36.0	32.0	36.0
26	34.574606793842364	36.0	36.0	36.0	32.0	36.0
27	34.54387758110645	36.0	36.0	36.0	32.0	36.0
28	34.507253142537024	36.0	36.0	36.0	32.0	36.0
29	34.46360979796689	36.0	36.0	36.0	32.0	36.0
30	34.436204123218246	36.0	36.0	36.0	32.0	36.0
31	34.429404711594216	36.0	36.0	36.0	32.0	36.0
32	34.38288173184339	36.0	36.0	36.0	32.0	36.0
33	34.32001133508274	36.0	36.0	36.0	32.0	36.0
34	34.28573089989418	36.0	36.0	36.0	32.0	36.0
35	34.23016606223812	36.0	36.0	36.0	32.0	36.0
36	34.19372508165518	36.0	36.0	36.0	32.0	36.0
37	34.166810812227475	36.0	36.0	36.0	32.0	36.0
38	33.73051168398052	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	7.0
21	26.0
22	67.0
23	185.0
24	497.0
25	1107.0
26	2396.0
27	4787.0
28	8053.0
29	12545.0
30	18329.0
31	26355.0
32	36701.0
33	57885.0
34	136792.0
35	304758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63023987654786	18.358427295765832	12.123406269913946	25.887926557772357
2	15.646836823949128	21.217079938541573	36.203631812925266	26.932451424584023
3	17.891412528214858	25.508358804509136	28.80781793105256	27.79241073622345
4	11.866966533604808	16.182658933026257	35.794349812364764	36.15602472100417
5	13.842887890790081	37.29307740944219	32.842583219491104	16.021451480276628
6	33.6350025880765	35.925941699481726	16.65820354730283	13.780852165138938
7	29.421303765972745	31.157114004583182	21.198441259768742	18.223140969675327
8	28.727628333022103	31.895289295998897	19.702792219918035	19.674290151060962
9	27.807454219924548	13.88677304319765	18.618677648315323	39.68709508856247
10	16.113640049206456	26.49826614402553	31.007929708807204	26.38016409796082
11	37.509562414102085	21.154762860320773	22.036388773766795	19.299285951810347
12	24.66445206668698	24.310967839159762	28.343439902767265	22.681140191385992
13	29.75852342529165	19.156453626079863	25.603855238544522	25.481167710083962
14	24.223989097353947	19.710267422775654	25.060688557135695	31.005054922734704
15	25.35864398339705	26.581751827208784	23.331269430985397	24.728334758408764
16	25.84419477373205	25.500538089707824	23.96571295657772	24.689554179982405
17	23.780086290774356	25.422690476892484	25.722283920890295	25.07493931144286
18	25.253810848263864	23.72029864339371	27.164722339613494	23.86116816872893
19	25.280674339141747	25.051843261358837	26.22187933050939	23.44560306899003
20	25.59058729487923	23.16091558639397	26.358817613277118	24.889679505449685
21	27.36100168224697	23.744416397894817	24.69856329229	24.19601862756821
22	25.616304173341586	23.97550180673356	25.591078700200164	24.81711531972468
23	23.73557108762918	23.60911591123895	26.323479548496053	26.331833452635824
24	24.346676625814503	25.173547979177517	25.773062470720433	24.706712924287544
25	24.880179002578238	23.716367400826215	25.919009851038666	25.484443745556877
26	24.185371494841053	24.738203386443416	26.57999354619955	24.496431572515984
27	25.422199071571548	24.438241817282396	25.608933093527536	24.530626017618516
28	24.175995177675784	23.946836496345583	26.83875681005874	25.038411515919893
29	24.04872119955315	24.724239714067625	26.818117786579393	24.408921299799836
30	24.335046699885666	24.548644212719537	26.981755758451353	24.134553328943444
31	24.425629080711687	24.551265041097864	25.550292058562412	25.472813819628037
32	24.234144807319975	24.69491919658506	25.512781452397565	25.5581545436974
33	23.81235524018254	24.26444813544441	26.241699345120512	25.68149727925254
34	24.594344907566658	24.507038562213552	26.638099637342872	24.260516892876915
35	26.03530911032705	24.05871310774553	26.156194819277502	23.74978296264992
36	24.299337913230925	25.10720826085105	25.773062470720433	24.820391355197593
37	25.430552962027477	25.101475198773453	25.642184853577593	23.82578698562148
38	24.220385458333745	24.640864611282012	26.127857112436814	25.010892817947433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	580.0
1	392.5
2	205.0
3	205.0
4	674.5
5	1144.0
6	1144.0
7	1260.5
8	1377.0
9	1324.5
10	1272.0
11	1272.0
12	1557.5
13	1843.0
14	2277.0
15	2711.0
16	2711.0
17	4009.5
18	5308.0
19	5308.0
20	5669.0
21	6030.0
22	5739.5
23	5449.0
24	5449.0
25	5727.5
26	6006.0
27	6006.0
28	7582.5
29	9159.0
30	11007.0
31	12855.0
32	12855.0
33	16346.0
34	19837.0
35	19837.0
36	21294.5
37	22752.0
38	26577.5
39	30403.0
40	30403.0
41	32755.5
42	35108.0
43	40675.5
44	46243.0
45	46243.0
46	50431.0
47	54619.0
48	54619.0
49	56113.0
50	57607.0
51	56543.5
52	55480.0
53	55480.0
54	52734.0
55	49988.0
56	49988.0
57	48830.0
58	47672.0
59	43035.5
60	38399.0
61	38399.0
62	37156.0
63	35913.0
64	29686.0
65	23459.0
66	23459.0
67	19598.5
68	15738.0
69	15738.0
70	12739.0
71	9740.0
72	7500.0
73	5260.0
74	5260.0
75	3877.5
76	2495.0
77	2495.0
78	2492.5
79	2490.0
80	1919.5
81	1349.0
82	1349.0
83	1320.5
84	1292.0
85	1292.0
86	841.0
87	390.0
88	322.0
89	254.0
90	254.0
91	150.0
92	46.0
93	29.0
94	12.0
95	12.0
96	8.0
97	4.0
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009009097550508277
2	0.0
3	0.0
4	1.6380177364560503E-4
5	0.0
6	3.2760354729121006E-4
7	1.6380177364560503E-4
8	0.0019656212837472604
9	0.0034398372465577055
10	8.190088682280251E-4
11	0.005077854983013756
12	0.0
13	0.0
14	0.0
15	0.0
16	1.6380177364560503E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.6380177364560503E-4
22	0.0
23	1.6380177364560503E-4
24	0.0
25	0.0
26	1.6380177364560503E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	610494.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.736823116767575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11572994623292	41.44933349098971
2	12.420101877617862	12.851532278690964
3	3.3734664512173307	5.235973112309419
4	1.3082395169762144	2.7073662593665544
5	0.6716257745701949	1.7373891949800095
6	0.4092406250980845	1.2703685899736994
7	0.26723168535267683	0.967800290540099
8	0.19569912261787492	0.8099880712790083
9	0.13149224610477958	0.6122691970154506
>10	0.8421626412366415	8.576350009931812
>50	0.12519662585466615	4.63404684916745
>100	0.1280564438856541	13.41650900684491
>500	0.00985049568351183	3.5827224701160483
>1k	0.0019065475516474508	2.148351178794866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3584	0.5870655567458485	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2907	0.47617175598777384	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1857	0.3041798936598885	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1760	0.28829112161626486	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1646	0.26961771942066587	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1314	0.21523553057032505	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	936	0.1533184601322863	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	932	0.1526632530377039	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	916	0.15004242465937423	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	886	0.14512837145000604	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	875	0.1433265519399044	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	836	0.1369382827677258	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	829	0.13579167035220657	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	789	0.12923959940638235	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	788	0.12907579763273677	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	757	0.12399794264972301	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	739	0.12104951072410212	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	733	0.12006670008222849	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	712	0.11662686283567078	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	708	0.11597165574108836	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	686	0.11236801672088505	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	678	0.11105760253172021	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	675	0.11056619721078341	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	649	0.10630735109599766	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	639	0.10466933335954161	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	624	0.10221230675485754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.6380177364560503E-4
13	0.0	0.0	0.0	0.0	1.6380177364560503E-4
14	0.0	0.0	0.0	0.0	1.6380177364560503E-4
15	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
16	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
17	0.0	0.0	0.0	1.6380177364560503E-4	3.2760354729121006E-4
18	0.0	0.0	0.0	3.2760354729121006E-4	3.2760354729121006E-4
19	0.0	0.0	0.0	3.2760354729121006E-4	3.2760354729121006E-4
20	0.0	0.0	0.0	3.2760354729121006E-4	3.2760354729121006E-4
21	0.0	0.0	0.0	4.914053209368151E-4	3.2760354729121006E-4
22	0.0	0.0	0.0	8.190088682280251E-4	3.2760354729121006E-4
23	0.0	0.0	0.0	0.0029484319256208908	3.2760354729121006E-4
24	0.0	0.0	0.0	0.005733062077596177	3.2760354729121006E-4
25	0.0	0.0	0.0	0.006060665624887386	3.2760354729121006E-4
26	0.0	0.0	0.0	0.0076986833613434365	4.914053209368151E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGTT	40	1.5916671E-4	24.001537	7
TAAGACT	40	1.5916671E-4	24.001537	4
ATATTAT	50	2.7952212E-5	22.3996	1
TAGGACG	55	5.8246846E-5	20.364939	4
AAGGCCG	40	0.0044794534	20.00128	5
TACCGTC	170	0.0	19.76597	7
CGTCGTA	180	0.0	19.555206	10
ACCCGTT	90	2.033994E-8	19.555206	30
GTCGAAG	50	7.1915204E-4	19.199656	1
AATGCCG	50	7.1915204E-4	19.199656	18
CCGTCGT	170	0.0	18.824734	9
ACCGTCG	170	0.0	18.824734	8
GTGTATA	80	2.7923033E-6	17.999678	1
ATACCGT	205	0.0	17.952368	6
CTATATG	45	0.008842393	17.778915	3
TAATACT	135	2.3646862E-11	17.778913	4
GTTTAAG	45	0.008846566	17.77746	1
TCGGCAA	45	0.008846566	17.77746	13
TAACCCG	90	4.3592627E-7	17.77746	28
GTCGTAG	180	0.0	17.77746	11
>>END_MODULE