FastQCFastQC Report
Thu 2 Feb 2017
SRR4063073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259539
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27060.21484050910690342No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17160.1362403228482802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16570.1315560693237764No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16140.128142121839816No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13990.11107238442001398No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13540.10749964868098567No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13510.10726146629838378No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13470.10694388978824791No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13400.10638813089551018No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12900.10241842451881204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT453.5377394E-421.33269912
AGTCCGA507.197905E-419.19942710
CTAGCGG3050.018.88468229
TTGTCGG701.7879387E-518.2851722
GTATCAA19150.017.0621681
TCTAGCG3300.016.9691928
CCTATTC4250.016.9393253
ACACCGT1151.8437277E-816.6938296
TATACTG1354.4383341E-1016.590785
CTATTCC4000.016.3982094
TAGCGGC3450.016.231430
GTCCTAT4550.016.1927011
GTCGTTC600.002442565715.9995238
CCGCCTA600.002442565715.99952326
CAAGACG3350.015.7594734
CGAGCCG4150.015.42122715
AACCGCG1255.1395546E-815.3595427
CCTTATA1153.337591E-715.3075382
TGTGCGA951.4330119E-515.15744410
TGTCGGG951.4330119E-515.15744423
Produced by FastQC (version 0.11.5)