##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063073_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259539
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233621983916336	32.0	32.0	32.0	32.0	32.0
2	30.783074601104055	32.0	32.0	32.0	32.0	32.0
3	30.82513681593027	32.0	32.0	32.0	32.0	32.0
4	30.912890351152285	32.0	32.0	32.0	32.0	32.0
5	30.78568666789992	32.0	32.0	32.0	32.0	32.0
6	34.450437818916285	36.0	36.0	36.0	32.0	36.0
7	34.3915043519891	36.0	36.0	36.0	32.0	36.0
8	34.36329164876991	36.0	36.0	36.0	32.0	36.0
9	34.535442729443076	36.0	36.0	36.0	32.0	36.0
10	34.209941891438056	36.0	36.0	36.0	32.0	36.0
11	34.506929916421804	36.0	36.0	36.0	32.0	36.0
12	34.316916744936044	36.0	36.0	36.0	32.0	36.0
13	34.415539336217456	36.0	36.0	36.0	32.0	36.0
14	34.295665318819026	36.0	36.0	36.0	32.0	36.0
15	34.23440957366147	36.0	36.0	36.0	32.0	36.0
16	34.24841707958229	36.0	36.0	36.0	32.0	36.0
17	34.178837654094075	36.0	36.0	36.0	32.0	36.0
18	34.205691923791164	36.0	36.0	36.0	32.0	36.0
19	34.19957778202978	36.0	36.0	36.0	32.0	36.0
20	34.19227272835538	36.0	36.0	36.0	32.0	36.0
21	34.1784954654044	36.0	36.0	36.0	32.0	36.0
22	34.151766638428825	36.0	36.0	36.0	32.0	36.0
23	34.086985000067486	36.0	36.0	36.0	32.0	36.0
24	34.06874658109038	36.0	36.0	36.0	32.0	36.0
25	34.03710246367917	36.0	36.0	36.0	32.0	36.0
26	34.003958591198845	36.0	36.0	36.0	32.0	36.0
27	33.997482412215895	36.0	36.0	36.0	32.0	36.0
28	33.99287120128873	36.0	36.0	36.0	32.0	36.0
29	33.9626506205842	36.0	36.0	36.0	32.0	36.0
30	33.9309803031109	36.0	36.0	36.0	32.0	36.0
31	33.959838480586946	36.0	36.0	36.0	32.0	36.0
32	33.901237675054126	36.0	36.0	36.0	32.0	36.0
33	33.87300909300943	36.0	36.0	36.0	32.0	36.0
34	33.88627346989652	36.0	36.0	36.0	32.0	36.0
35	33.850527057915635	36.0	36.0	36.0	32.0	36.0
36	33.80086206143677	36.0	36.0	36.0	32.0	36.0
37	33.801473396218775	36.0	36.0	36.0	32.0	36.0
38	33.20580625133481	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	6.0
10	14.0
11	16.0
12	7.0
13	14.0
14	93.0
15	236.0
16	334.0
17	421.0
18	510.0
19	668.0
20	948.0
21	1488.0
22	2378.0
23	3716.0
24	5785.0
25	8829.0
26	12843.0
27	18658.0
28	26032.0
29	35043.0
30	47355.0
31	63878.0
32	86729.0
33	128466.0
34	276015.0
35	539051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.7122760664317	16.71094069188299	11.032339910052984	25.544443331632323
2	16.99969337288241	18.875997133910257	37.21031283909702	26.91399665411031
3	19.419429990717678	23.049461522031027	28.041429811264724	29.489678675986575
4	12.732960432554316	14.784258770380193	35.67564777946717	36.807133017598325
5	14.894917735644132	36.205923287684286	33.48047735001036	15.418681626661227
6	35.052404177090466	35.07542851063526	16.41444435534645	13.457722956927823
7	30.974824916100257	30.018919620591344	20.286708073350646	18.719547389957754
8	28.53399982215673	32.81930806265164	19.07083243353108	19.57585968166055
9	27.401519289643172	14.139333849925686	18.344215647667077	40.11493121276406
10	15.95899753400861	26.350380378775007	31.334684097451916	26.355937989764467
11	38.104103976943414	21.080578955308017	21.679700836039412	19.135616231709157
12	24.85361242843385	23.4810031607806	28.41606067174329	23.24932373904227
13	29.53642552387275	18.978927566636994	25.20330367983432	26.281343229655935
14	23.558193159873973	19.533393891655656	24.815069493851002	32.09334345461937
15	25.33816703263541	26.895168815492195	22.05103973507304	25.715624416799354
16	26.15077379959821	25.61248878407458	23.31864344870848	24.91809396761873
17	24.288199917886782	25.781246897923104	24.811135632132792	25.119417552057318
18	25.32617464158901	24.660565581111726	25.748740718155886	24.26451905914337
19	25.6784032116167	24.772356974753617	25.1985822135128	24.35065760011688
20	25.730657979255977	24.188552594908717	24.86305497571553	25.217734450119778
21	27.054722733913483	23.91427936225624	24.395683795972815	24.63531410785746
22	25.87297428201647	24.01411749759812	24.99376702158913	25.119141198796278
23	24.4496214882254	23.913575129862807	25.58523621453288	26.05156716737891
24	24.95755666126166	24.793500650347884	25.082623562127676	25.166319126262778
25	24.970240907694006	24.389908828782183	25.105715254522625	25.53413500900118
26	24.814714521791544	25.068114697924916	25.483036640982537	24.634134139301008
27	25.589939408087442	24.556597578705567	24.673398395596667	25.180064617610327
28	24.677397327709688	24.482073041754933	25.532933580214763	25.30759605032062
29	24.66576621858655	24.77891776304558	25.44099325616795	25.11432276219992
30	24.475698816818927	25.037594701330544	25.701754525258718	24.784951956591815
31	25.29022566181614	24.76991959194919	24.587617938292162	25.352236807942507
32	25.02251004019253	24.636464835903	24.58223429815521	25.758790825749262
33	24.612179204690456	24.22072575311118	25.248191810603227	25.918903231595138
34	25.504364528882594	24.561661228706395	25.154662260185155	24.77931198222585
35	25.89363917107448	24.261722822768213	25.348264178121948	24.496373828035356
36	24.579731504612827	25.012189176011702	24.96517945484605	25.442899864529416
37	25.710619551943235	24.76095646001172	24.78382460135589	24.744599386689153
38	24.91930995220926	24.455297515024427	25.24548433481626	25.37990819795006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	70.0
2	101.0
3	101.0
4	311.0
5	521.0
6	521.0
7	613.5
8	706.0
9	686.0
10	666.0
11	666.0
12	947.0
13	1228.0
14	1782.0
15	2336.0
16	2336.0
17	3449.0
18	4562.0
19	4562.0
20	5854.0
21	7146.0
22	7832.5
23	8519.0
24	8519.0
25	10119.5
26	11720.0
27	11720.0
28	14797.0
29	17874.0
30	21808.5
31	25743.0
32	25743.0
33	32997.0
34	40251.0
35	40251.0
36	45283.5
37	50316.0
38	57980.5
39	65645.0
40	65645.0
41	71167.5
42	76690.0
43	87244.5
44	97799.0
45	97799.0
46	101209.0
47	104619.0
48	104619.0
49	111135.5
50	117652.0
51	120308.5
52	122965.0
53	122965.0
54	117908.5
55	112852.0
56	112852.0
57	108609.5
58	104367.0
59	93752.0
60	83137.0
61	83137.0
62	77997.5
63	72858.0
64	60716.0
65	48574.0
66	48574.0
67	40721.0
68	32868.0
69	32868.0
70	26110.0
71	19352.0
72	14903.0
73	10454.0
74	10454.0
75	7859.0
76	5264.0
77	5264.0
78	4977.5
79	4691.0
80	3646.5
81	2602.0
82	2602.0
83	2630.5
84	2659.0
85	2659.0
86	1782.0
87	905.0
88	783.0
89	661.0
90	661.0
91	466.0
92	271.0
93	244.0
94	217.0
95	217.0
96	190.5
97	164.0
98	164.0
99	354.5
100	545.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11631239683725554
2	0.05406740085062868
3	0.0123854838952982
4	0.0034933416114943643
5	1.5878825506792564E-4
6	1.5878825506792564E-4
7	0.0
8	2.3818238260188846E-4
9	2.3818238260188846E-4
10	3.969706376698141E-4
11	0.0023024296984849217
12	0.003175765101358513
13	0.01714913154733597
14	0.011670936747492535
15	0.025723697321003956
16	0.013417607553239716
17	0.023977026515256773
18	0.007224865605590616
19	0.008812748156269874
20	0.00730425973312458
21	0.007860018625862318
22	0.008653959901201947
23	0.010559418962017056
24	0.016910949164734082
25	0.020642473158830332
26	0.02111883792403411
27	0.010321236579415166
28	0.007542442115726467
29	0.013179425170637828
30	0.00420788875930003
31	0.006986683222988729
32	0.008018806880930245
33	0.010321236579415166
34	0.014449731211181233
35	0.01762549631253975
36	0.017863678695141636
37	0.011512148492424608
38	0.007145471478056655
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1259539.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.042988785494124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57584054668555	48.164814385652114
2	11.592981340776149	13.689685345877775
3	3.0758945871164154	5.448300288374298
4	1.2558482174710022	2.9659612888169264
5	0.6510122887880495	1.9218855633065832
6	0.3892268372761347	1.3788669472984902
7	0.26797663760709317	1.1075499126307018
8	0.1770196270645604	0.8361414284468153
9	0.13818131856761964	0.7342774238297481
>10	0.7387677560053124	7.8959016118919205
>50	0.06299018748644734	2.6133870008509366
>100	0.06467429017126462	8.302460419742557
>500	0.006885980481836924	2.8162467476026998
>1k	0.002700384502681147	2.1245216356784313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2706	0.21484050910690342	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1716	0.1362403228482802	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1657	0.1315560693237764	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1614	0.128142121839816	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1399	0.11107238442001398	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1354	0.10749964868098567	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1351	0.10726146629838378	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1347	0.10694388978824791	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1340	0.10638813089551018	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1290	0.10241842451881204	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3818238260188846E-4
2	0.0	0.0	0.0	0.0	2.3818238260188846E-4
3	0.0	0.0	0.0	0.0	2.3818238260188846E-4
4	0.0	0.0	0.0	0.0	2.3818238260188846E-4
5	0.0	0.0	0.0	0.0	2.3818238260188846E-4
6	0.0	0.0	0.0	0.0	3.175765101358513E-4
7	0.0	0.0	0.0	0.0	3.175765101358513E-4
8	0.0	0.0	0.0	0.0	3.175765101358513E-4
9	0.0	0.0	0.0	0.0	3.175765101358513E-4
10	0.0	0.0	0.0	0.0	3.175765101358513E-4
11	0.0	0.0	0.0	0.0	3.175765101358513E-4
12	0.0	7.939412753396282E-5	0.0	7.939412753396282E-5	6.351530202717026E-4
13	0.0	7.939412753396282E-5	0.0	7.939412753396282E-5	6.351530202717026E-4
14	0.0	7.939412753396282E-5	0.0	7.939412753396282E-5	6.351530202717026E-4
15	0.0	7.939412753396282E-5	0.0	7.939412753396282E-5	0.0011115177854754796
16	0.0	7.939412753396282E-5	0.0	2.3818238260188846E-4	0.0011115177854754796
17	0.0	7.939412753396282E-5	0.0	2.3818238260188846E-4	0.0011115177854754796
18	0.0	1.5878825506792564E-4	0.0	3.969706376698141E-4	0.0011909119130094424
19	0.0	1.5878825506792564E-4	0.0	3.969706376698141E-4	0.0012703060405434051
20	0.0	3.175765101358513E-4	0.0	3.969706376698141E-4	0.001349700168077368
21	0.0	3.175765101358513E-4	0.0	3.969706376698141E-4	0.001349700168077368
22	0.0	3.175765101358513E-4	0.0	4.763647652037769E-4	0.0014290942956113309
23	0.0	3.175765101358513E-4	0.0	9.527295304075538E-4	0.0015084884231452936
24	0.0	3.175765101358513E-4	0.0	0.0015878825506792564	0.0015084884231452936
25	0.0	3.175765101358513E-4	0.0	0.0017466708057471822	0.0015084884231452936
26	0.0	3.175765101358513E-4	0.0	0.0025406120810868103	0.0015878825506792564
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAT	45	3.5377394E-4	21.332699	12
AGTCCGA	50	7.197905E-4	19.199427	10
CTAGCGG	305	0.0	18.884682	29
TTGTCGG	70	1.7879387E-5	18.28517	22
GTATCAA	1915	0.0	17.062168	1
TCTAGCG	330	0.0	16.96919	28
CCTATTC	425	0.0	16.939325	3
ACACCGT	115	1.8437277E-8	16.693829	6
TATACTG	135	4.4383341E-10	16.59078	5
CTATTCC	400	0.0	16.398209	4
TAGCGGC	345	0.0	16.2314	30
GTCCTAT	455	0.0	16.192701	1
GTCGTTC	60	0.0024425657	15.999523	8
CCGCCTA	60	0.0024425657	15.999523	26
CAAGACG	335	0.0	15.759473	4
CGAGCCG	415	0.0	15.421227	15
AACCGCG	125	5.1395546E-8	15.359542	7
CCTTATA	115	3.337591E-7	15.307538	2
TGTGCGA	95	1.4330119E-5	15.157444	10
TGTCGGG	95	1.4330119E-5	15.157444	23
>>END_MODULE