FastQCFastQC Report
Thu 2 Feb 2017
SRR4063073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259539
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24090.19126045322931645No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17970.1426712471785312No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16740.13290576949185376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15690.12456938610078767No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14420.11448633190397439No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13990.11107238442001398No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13940.11067541378234419No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13850.10996086663453851No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13180.104641460089763No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12680.10067175371306486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA1600.018.0005369
CGATAAC1600.018.00053610
TCTAGCG3200.017.49982628
CGGTCCA3650.017.096410
TAACGAA1505.456968E-1217.06717513
CTAGCGG3200.016.99983229
GTAGTAA957.691433E-716.8426061
GTGTAGG1456.91216E-1116.5522161
ACGAACG1651.8189894E-1216.48468415
TAGGACG908.582912E-615.9998434
TAGCGGC3500.015.99984230
CTAGATA2850.015.7191433
GCCGGTC4100.015.6102218
CGCTTCG3300.015.514997532
ACCGTCG3000.015.4671278
GTATCAA18750.015.2751221
TCTAGAT3150.015.2379452
CGCCGGT4000.015.2004527
GTAAACG3500.015.08556527
GTACCGT859.4309326E-515.05927311
Produced by FastQC (version 0.11.5)