##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259539
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.154110353073623	32.0	32.0	32.0	32.0	32.0
2	31.17425740687664	32.0	32.0	32.0	32.0	32.0
3	31.30812384531166	32.0	32.0	32.0	32.0	32.0
4	31.409179072660713	32.0	32.0	32.0	32.0	32.0
5	31.335452097950125	32.0	32.0	32.0	32.0	32.0
6	34.840683773983976	36.0	36.0	36.0	32.0	36.0
7	34.872186569848175	36.0	36.0	36.0	32.0	36.0
8	34.78913316697617	36.0	36.0	36.0	32.0	36.0
9	34.93529616788365	36.0	36.0	36.0	32.0	36.0
10	34.74966317041394	36.0	36.0	36.0	32.0	36.0
11	34.943164919863534	36.0	36.0	36.0	36.0	36.0
12	34.83267052469197	36.0	36.0	36.0	32.0	36.0
13	34.903215382770995	36.0	36.0	36.0	32.0	36.0
14	34.8358955141524	36.0	36.0	36.0	32.0	36.0
15	34.80459358543086	36.0	36.0	36.0	32.0	36.0
16	34.82571321729617	36.0	36.0	36.0	32.0	36.0
17	34.786717203675316	36.0	36.0	36.0	32.0	36.0
18	34.78106037208852	36.0	36.0	36.0	32.0	36.0
19	34.78092698995426	36.0	36.0	36.0	32.0	36.0
20	34.77286451630319	36.0	36.0	36.0	32.0	36.0
21	34.769123465013784	36.0	36.0	36.0	32.0	36.0
22	34.75168692672478	36.0	36.0	36.0	32.0	36.0
23	34.68076415259869	36.0	36.0	36.0	32.0	36.0
24	34.67031826723905	36.0	36.0	36.0	32.0	36.0
25	34.65292777754401	36.0	36.0	36.0	32.0	36.0
26	34.57886178990885	36.0	36.0	36.0	32.0	36.0
27	34.57337803752008	36.0	36.0	36.0	32.0	36.0
28	34.54119642186546	36.0	36.0	36.0	32.0	36.0
29	34.500749083593284	36.0	36.0	36.0	32.0	36.0
30	34.480153452969695	36.0	36.0	36.0	32.0	36.0
31	34.486757456497976	36.0	36.0	36.0	32.0	36.0
32	34.442931898099225	36.0	36.0	36.0	32.0	36.0
33	34.41993618300029	36.0	36.0	36.0	32.0	36.0
34	34.39688251018825	36.0	36.0	36.0	32.0	36.0
35	34.36811404807632	36.0	36.0	36.0	32.0	36.0
36	34.347310404838595	36.0	36.0	36.0	32.0	36.0
37	34.34641563302129	36.0	36.0	36.0	32.0	36.0
38	33.907626520496784	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	33.0
22	100.0
23	280.0
24	844.0
25	2132.0
26	4459.0
27	8831.0
28	15326.0
29	24574.0
30	37191.0
31	53906.0
32	78290.0
33	125466.0
34	298132.0
35	609972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.157499396541866	18.281287716133292	11.976817679417632	26.584395207907207
2	15.91399710529011	20.216126693972953	36.49065253239479	27.379223668342146
3	18.219840751258992	23.89040752211722	28.442231641894377	29.447520084729412
4	12.113737031941103	15.775796979366227	35.74948572372229	36.36098026497038
5	14.293097945437136	36.84739960207632	33.085702852950845	15.773799599535703
6	35.002119824888176	35.51206871090828	16.112098245547177	13.373713218656363
7	30.66845463235551	30.211697678185228	20.34192064989218	18.77792703956708
8	28.449588645635004	33.11279573290311	18.837505101238747	19.600110520223136
9	27.20633655316534	13.95998049998174	18.087934284303277	40.745748662549644
10	15.689168535757528	26.714674182727617	31.272900498203686	26.323256783311166
11	38.39741543718906	20.959794454527987	21.487235187911182	19.155554920371774
12	24.5919132124846	23.573045947078924	28.374337851399247	23.460702989037234
13	29.527857877821674	19.01065154152005	25.096225911764332	26.36526466889394
14	23.46247317470916	19.402416280877368	24.743814998979786	32.391295545433685
15	25.287823560842497	27.059265334380278	21.872764559096623	25.780146545680605
16	26.135594054650152	25.806981760787085	23.11623538453355	24.941188800029217
17	24.372647452758507	25.909479579433427	24.61384681220669	25.10402615560137
18	25.350147950956657	24.72142585501521	25.5737218140923	24.354704379935836
19	25.74283130573964	24.93007362217446	24.985252540810563	24.341842531275333
20	25.832308487470417	24.275389646529405	24.700624593601308	25.19167727239887
21	27.12592008015644	24.05288610020984	24.252959619288674	24.56823420034505
22	25.940522683299207	24.137561441130444	24.82630549748757	25.09561037808277
23	24.430684559985835	23.94090218722882	25.538629609722285	26.089783643063058
24	24.91737056176903	24.90323840706798	24.91093963743878	25.268451393724213
25	25.039974974951136	24.468019226097187	25.025445838077136	25.46655996087454
26	24.90220628341004	25.124430446377605	25.282504154297726	24.69085911591463
27	25.606749771146426	24.48848348483056	24.640443844930566	25.264322899092445
28	24.62265956036296	24.521829018394826	25.511873788743344	25.343637632498872
29	24.69617852245941	24.80518665956354	25.34975098031899	25.148883837658065
30	24.57414974843971	25.016930797696617	25.573086661072026	24.83583279279165
31	25.30330541571162	24.707373094441696	24.499519268557783	25.489802221288897
32	25.020900504073317	24.69355851625079	24.47284284170637	25.812698137969527
33	24.538739967559557	24.333823724394403	25.154044455947773	25.973391852098267
34	25.408502634694123	24.49761380949697	25.161507503935965	24.932376051872946
35	25.970533663507045	24.321120663988967	25.118475886812558	24.58986978569143
36	24.68895365685382	25.087432782946777	24.738971957200214	25.48464160299919
37	25.8007096247119	24.75207198824332	24.692288210210243	24.75493017683454
38	24.82162124396307	24.459584022408198	25.17786269420796	25.54093203942077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	34.0
2	44.0
3	44.0
4	186.5
5	329.0
6	329.0
7	416.5
8	504.0
9	549.5
10	595.0
11	595.0
12	832.5
13	1070.0
14	1605.5
15	2141.0
16	2141.0
17	3228.0
18	4315.0
19	4315.0
20	5568.0
21	6821.0
22	7635.0
23	8449.0
24	8449.0
25	10191.0
26	11933.0
27	11933.0
28	15025.5
29	18118.0
30	22399.5
31	26681.0
32	26681.0
33	33637.0
34	40593.0
35	40593.0
36	45853.0
37	51113.0
38	58533.0
39	65953.0
40	65953.0
41	71539.5
42	77126.0
43	87867.5
44	98609.0
45	98609.0
46	101412.5
47	104216.0
48	104216.0
49	111534.0
50	118852.0
51	120991.0
52	123130.0
53	123130.0
54	117681.0
55	112232.0
56	112232.0
57	108543.5
58	104855.0
59	94164.0
60	83473.0
61	83473.0
62	78156.5
63	72840.0
64	60631.5
65	48423.0
66	48423.0
67	40292.5
68	32162.0
69	32162.0
70	25658.5
71	19155.0
72	14800.0
73	10445.0
74	10445.0
75	7753.0
76	5061.0
77	5061.0
78	4800.5
79	4540.0
80	3528.5
81	2517.0
82	2517.0
83	2318.0
84	2119.0
85	2119.0
86	1354.5
87	590.0
88	488.0
89	386.0
90	386.0
91	225.5
92	65.0
93	50.5
94	36.0
95	36.0
96	24.0
97	12.0
98	12.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01040063070694913
2	0.0
3	0.0
4	1.5878825506792564E-4
5	7.939412753396282E-5
6	7.939412753396282E-5
7	5.557588927377397E-4
8	0.0032551592288924754
9	0.00420788875930003
10	0.0011115177854754794
11	0.004843041779571732
12	2.3818238260188846E-4
13	2.3818238260188846E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5878825506792564E-4
22	0.0
23	0.0
24	0.0
25	7.939412753396282E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1259539.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.160971145604236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21596372696398	45.85222388013824
2	12.359288559185275	14.12937873423572
3	3.3612732251490716	5.764009255057146
4	1.3775860634713721	3.149766288986945
5	0.685155345442079	1.9582072465535592
6	0.40389178539717174	1.385210801462059
7	0.29099694446341057	1.1643567562152406
8	0.20292193729209576	0.9279372001890879
9	0.15404323084947102	0.7924734606386038
>10	0.801292807769452	8.244701346209077
>50	0.06803520199883911	2.7285714431926893
>100	0.06740916230273446	8.146385567461238
>500	0.009211179783875284	3.5436390293134985
>1k	0.002930829931233045	2.21313899034703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2409	0.19126045322931645	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1797	0.1426712471785312	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1674	0.13290576949185376	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1569	0.12456938610078767	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1442	0.11448633190397439	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1399	0.11107238442001398	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1394	0.11067541378234419	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1385	0.10996086663453851	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1318	0.104641460089763	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1268	0.10067175371306486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.3818238260188846E-4
7	0.0	0.0	0.0	0.0	2.3818238260188846E-4
8	0.0	0.0	0.0	0.0	2.3818238260188846E-4
9	0.0	0.0	0.0	0.0	2.3818238260188846E-4
10	0.0	0.0	0.0	0.0	2.3818238260188846E-4
11	0.0	0.0	0.0	0.0	2.3818238260188846E-4
12	0.0	0.0	0.0	1.5878825506792564E-4	3.969706376698141E-4
13	0.0	0.0	0.0	1.5878825506792564E-4	3.969706376698141E-4
14	0.0	0.0	0.0	1.5878825506792564E-4	3.969706376698141E-4
15	0.0	0.0	0.0	1.5878825506792564E-4	7.145471478056654E-4
16	0.0	0.0	0.0	1.5878825506792564E-4	7.145471478056654E-4
17	0.0	0.0	0.0	1.5878825506792564E-4	7.145471478056654E-4
18	0.0	0.0	0.0	1.5878825506792564E-4	8.733354028735911E-4
19	0.0	0.0	0.0	2.3818238260188846E-4	8.733354028735911E-4
20	0.0	0.0	0.0	2.3818238260188846E-4	0.0010321236579415166
21	0.0	0.0	0.0	3.175765101358513E-4	0.0010321236579415166
22	0.0	0.0	0.0	3.969706376698141E-4	0.0010321236579415166
23	0.0	7.939412753396282E-5	0.0	7.939412753396282E-4	0.0011115177854754796
24	0.0	7.939412753396282E-5	0.0	0.001349700168077368	0.0011909119130094424
25	0.0	7.939412753396282E-5	0.0	0.0015084884231452936	0.0011909119130094424
26	0.0	7.939412753396282E-5	0.0	0.002223035570950959	0.0012703060405434051
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	160	0.0	18.000536	9
CGATAAC	160	0.0	18.000536	10
TCTAGCG	320	0.0	17.499826	28
CGGTCCA	365	0.0	17.0964	10
TAACGAA	150	5.456968E-12	17.067175	13
CTAGCGG	320	0.0	16.999832	29
GTAGTAA	95	7.691433E-7	16.842606	1
GTGTAGG	145	6.91216E-11	16.552216	1
ACGAACG	165	1.8189894E-12	16.484684	15
TAGGACG	90	8.582912E-6	15.999843	4
TAGCGGC	350	0.0	15.999842	30
CTAGATA	285	0.0	15.719143	3
GCCGGTC	410	0.0	15.610221	8
CGCTTCG	330	0.0	15.5149975	32
ACCGTCG	300	0.0	15.467127	8
GTATCAA	1875	0.0	15.275122	1
TCTAGAT	315	0.0	15.237945	2
CGCCGGT	400	0.0	15.200452	7
GTAAACG	350	0.0	15.085565	27
GTACCGT	85	9.4309326E-5	15.059273	11
>>END_MODULE