FastQCFastQC Report
Thu 2 Feb 2017
SRR4063072_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063072_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848720
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26220.1418278592756069No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23180.12538404950452206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.019.9088921
GTATCAA16850.019.8626561
TCTAGCG2900.019.86129828
CTAGCGG2950.019.52519229
GTATTAC1700.016.9556541
TAGCGGC3500.016.91408730
AAGACGG5800.016.2725895
CAAGACG5750.016.1358834
GTCCTAC5800.015.7375761
TACCGTC3650.014.9027237
GCGCAAG5900.014.6565821
GACGGAC6050.014.5441027
AGCGGCG4100.014.43885431
TAGGACG2900.014.3419424
CGGTCCA4250.014.30609910
ACGTTTT1801.1823431E-1014.22205429
CGCCGGT3950.014.1758977
TCCTACA5550.014.1297812
GCCGGTC4650.014.10621558
CGCGTAA2500.014.08021410
Produced by FastQC (version 0.11.5)