##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063072_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1848720
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.149342247609155	32.0	32.0	32.0	32.0	32.0
2	30.7709252888485	32.0	32.0	32.0	32.0	32.0
3	30.810521874594315	32.0	32.0	32.0	32.0	32.0
4	30.858890475572288	32.0	32.0	32.0	32.0	32.0
5	30.768794625470594	32.0	32.0	32.0	32.0	32.0
6	34.23706510450474	36.0	36.0	36.0	32.0	36.0
7	34.236624799861524	36.0	36.0	36.0	32.0	36.0
8	34.19436799515341	36.0	36.0	36.0	32.0	36.0
9	34.40300424077199	36.0	36.0	36.0	32.0	36.0
10	34.05357166039205	36.0	36.0	36.0	32.0	36.0
11	34.37724209182569	36.0	36.0	36.0	32.0	36.0
12	34.161551776364185	36.0	36.0	36.0	32.0	36.0
13	34.28307693972045	36.0	36.0	36.0	32.0	36.0
14	34.15477627764075	36.0	36.0	36.0	32.0	36.0
15	34.07517958371197	36.0	36.0	36.0	32.0	36.0
16	34.1119369076983	36.0	36.0	36.0	32.0	36.0
17	34.026260331472585	36.0	36.0	36.0	32.0	36.0
18	34.06320102557445	36.0	36.0	36.0	32.0	36.0
19	34.03571011294301	36.0	36.0	36.0	32.0	36.0
20	34.01204400882773	36.0	36.0	36.0	32.0	36.0
21	34.00734886840625	36.0	36.0	36.0	32.0	36.0
22	33.98308072612402	36.0	36.0	36.0	32.0	36.0
23	33.9273962525423	36.0	36.0	36.0	32.0	36.0
24	33.913855532476525	36.0	36.0	36.0	32.0	36.0
25	33.8973051624908	36.0	36.0	36.0	32.0	36.0
26	33.844257648535205	36.0	36.0	36.0	32.0	36.0
27	33.84326182439742	36.0	36.0	36.0	32.0	36.0
28	33.82728320135012	36.0	36.0	36.0	32.0	36.0
29	33.79799536976935	36.0	36.0	36.0	32.0	36.0
30	33.760114024838806	36.0	36.0	36.0	32.0	36.0
31	33.791296680946814	36.0	36.0	36.0	32.0	36.0
32	33.75143450603661	36.0	36.0	36.0	32.0	36.0
33	33.70374258946731	36.0	36.0	36.0	32.0	36.0
34	33.72106809035441	36.0	36.0	36.0	32.0	36.0
35	33.695479034142544	36.0	36.0	36.0	32.0	36.0
36	33.65141178761522	36.0	36.0	36.0	27.0	36.0
37	33.664362369639534	36.0	36.0	36.0	27.0	36.0
38	32.88676056947509	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	3.0
9	9.0
10	13.0
11	13.0
12	3.0
13	10.0
14	154.0
15	369.0
16	643.0
17	827.0
18	985.0
19	1435.0
20	1873.0
21	2561.0
22	3585.0
23	5266.0
24	7551.0
25	11194.0
26	16917.0
27	24574.0
28	35914.0
29	50946.0
30	72715.0
31	104665.0
32	152217.0
33	243451.0
34	487965.0
35	622856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10388841748789	17.278762966761445	11.468689978724683	26.148658637025985
2	16.972728537110306	18.658544703000114	37.649641822917594	26.71908493697198
3	19.568295040764802	22.081858513598096	28.242426012871842	30.10742043276526
4	12.77309306138478	14.689971184892164	35.526912942889616	37.01002281083344
5	14.850847831684261	35.84449964191363	34.108808491947926	15.195844034454185
6	35.70079373922902	34.903754926386824	15.956462802872046	13.43898853151211
7	31.090933776306727	29.814590535392345	20.225518318359903	18.868957369941025
8	28.160555197948838	33.806275727399104	18.64559611837443	19.387572956277623
9	26.708295604403926	14.420510690133789	18.42962188851277	40.44157181694951
10	15.614480823624296	26.665718256056802	31.873195698398938	25.846605221919972
11	38.08093446083991	20.929354496530227	21.799596577235334	19.19011446539452
12	24.433731707237914	23.26338440034576	28.894369069423515	23.408514822992814
13	29.524322692577982	19.20313082410808	25.09257711423407	26.179969369079863
14	23.156722848863094	19.47111522480362	24.882003322570053	32.490158603763234
15	24.91167683622911	27.642201646246388	21.894460176397455	25.55166134112705
16	25.812671013895333	25.9250303213249	23.545631294853113	24.716667369926657
17	24.13252838011912	26.27314921263868	25.026604248348157	24.567718158894042
18	24.901007452029187	25.098559793662744	25.8575567557275	24.142875998580564
19	25.32461174525707	25.116345242030018	25.227186297903327	24.33185671480959
20	25.564695165227025	24.563293029133273	24.984312520556006	24.887699285083695
21	26.137045968692085	24.44436510538211	24.763849084120686	24.654739841805124
22	25.570192568806643	24.41449843285625	25.243348159079424	24.77196083925768
23	24.41166080892548	24.387317417804542	25.628235304300507	25.572786468969475
24	24.74947200318529	24.952015043581376	25.33356847945573	24.9649444737776
25	24.9136438170596	24.613446191915493	25.350171954974144	25.122738036050762
26	24.495488584316785	25.589394629581342	25.669733426674213	24.245383359427663
27	25.125774119418875	24.846258044134004	25.0926671254041	24.93530071104302
28	24.496598551138575	24.811697781701415	25.658489774015703	25.033213893144307
29	24.4414547169893	25.146276653294038	25.565631910814517	24.846636718902147
30	24.33932268099624	25.153366932244843	25.841807438965613	24.665502947793307
31	25.079599831658395	24.848130120706127	25.01955510645227	25.05271494118322
32	24.761550828405312	24.882993294428466	24.87303988073214	25.48241599643408
33	24.303936063114627	24.845867596248567	25.38877285343884	25.461423487197965
34	25.06673043069935	24.89604983981451	25.46051300472496	24.576706724761184
35	25.29847241848679	24.753031250608633	25.671062512037356	24.277433818867223
36	24.31829751414257	25.27561581111573	25.36401195133127	25.042074723410423
37	25.28833628341563	24.934759182401642	24.98885604608187	24.788048488100852
38	24.38465762020551	24.981702418850972	25.441562691867652	25.19207726907587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	34.0
2	64.0
3	64.0
4	217.0
5	370.0
6	370.0
7	458.0
8	546.0
9	539.0
10	532.0
11	532.0
12	793.0
13	1054.0
14	1808.0
15	2562.0
16	2562.0
17	4038.5
18	5515.0
19	5515.0
20	7333.5
21	9152.0
22	10928.0
23	12704.0
24	12704.0
25	15734.5
26	18765.0
27	18765.0
28	23698.5
29	28632.0
30	34930.5
31	41229.0
32	41229.0
33	51912.5
34	62596.0
35	62596.0
36	71641.5
37	80687.0
38	91285.5
39	101884.0
40	101884.0
41	110094.5
42	118305.0
43	131985.0
44	145665.0
45	145665.0
46	150368.0
47	155071.0
48	155071.0
49	163682.0
50	172293.0
51	176769.0
52	181245.0
53	181245.0
54	174313.0
55	167381.0
56	167381.0
57	159248.5
58	151116.0
59	136172.0
60	121228.0
61	121228.0
62	110432.0
63	99636.0
64	82968.0
65	66300.0
66	66300.0
67	54889.0
68	43478.0
69	43478.0
70	34213.5
71	24949.0
72	19360.5
73	13772.0
74	13772.0
75	10397.0
76	7022.0
77	7022.0
78	6343.5
79	5665.0
80	4489.5
81	3314.0
82	3314.0
83	3139.5
84	2965.0
85	2965.0
86	1954.0
87	943.0
88	810.5
89	678.0
90	678.0
91	477.0
92	276.0
93	244.0
94	212.0
95	212.0
96	187.0
97	162.0
98	162.0
99	455.0
100	748.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10704703795058203
2	0.04819550824354148
3	0.010223289627417888
4	0.0020013847418754597
5	2.1636591804059024E-4
6	1.0818295902029512E-4
7	5.409147951014756E-5
8	5.409147951014755E-4
9	3.2454887706088537E-4
10	7.031892336319183E-4
11	0.0021095677008957548
12	0.0020554762213856074
13	0.014117876152148514
14	0.008167813406032282
15	0.02131204292699814
16	0.010061015188887447
17	0.017904279717858844
18	0.005192782032974166
19	0.006869617897788741
20	0.006058245705136527
21	0.006166428664156822
22	0.0072482582543597724
23	0.009141460037214938
24	0.012116491410273053
25	0.014658790947249988
26	0.016119260894023973
27	0.00908736855770479
28	0.005192782032974166
29	0.00908736855770479
30	0.0026504824959972307
31	0.005300964991994461
32	0.0058418797870959365
33	0.00795144748799169
34	0.01287377212341512
35	0.016660175689125447
36	0.012062399930762906
37	0.009952832229867151
38	0.006220520143666969
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1848720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.23645004634916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85161490129055	41.89773937430049
2	13.863032686896712	15.314894250013516
3	4.61526976425854	7.647933533516775
4	2.061261236501365	4.554270132899344
5	1.0555970150219574	2.915371589466782
6	0.6211768592235833	2.058696273267092
7	0.39229577156410744	1.516831805257638
8	0.277855747137041	1.227821207746095
9	0.1983217633689176	0.9859131157887988
>10	0.9468365610754941	9.11812769427138
>50	0.05608682948384916	2.1647395664212064
>100	0.05180476339617238	6.178408440848654
>500	0.0059956905298270535	2.2398671028334918
>1k	0.0028504102518849927	2.1793859133687707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2622	0.1418278592756069	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2318	0.12538404950452206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.409147951014756E-5
2	0.0	0.0	0.0	0.0	5.409147951014756E-5
3	0.0	0.0	0.0	0.0	5.409147951014756E-5
4	0.0	0.0	0.0	0.0	5.409147951014756E-5
5	0.0	5.409147951014756E-5	0.0	0.0	5.409147951014756E-5
6	0.0	5.409147951014756E-5	0.0	0.0	5.409147951014756E-5
7	0.0	5.409147951014756E-5	0.0	0.0	5.409147951014756E-5
8	0.0	5.409147951014756E-5	0.0	0.0	5.409147951014756E-5
9	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	5.409147951014756E-5
10	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	5.409147951014756E-5
11	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	5.409147951014756E-5
12	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	3.2454887706088537E-4
13	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	4.868233155913281E-4
14	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	4.868233155913281E-4
15	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	5.409147951014756E-4
16	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	5.409147951014756E-4
17	0.0	5.409147951014756E-5	0.0	1.0818295902029512E-4	5.409147951014756E-4
18	0.0	5.409147951014756E-5	0.0	1.0818295902029512E-4	5.950062746116232E-4
19	0.0	5.409147951014756E-5	0.0	1.0818295902029512E-4	5.950062746116232E-4
20	0.0	5.409147951014756E-5	0.0	1.0818295902029512E-4	8.113721926522134E-4
21	0.0	5.409147951014756E-5	0.0	1.6227443853044268E-4	0.0010277381106928037
22	0.0	5.409147951014756E-5	0.0	1.6227443853044268E-4	0.0011359210697130988
23	0.0	5.409147951014756E-5	0.0	3.2454887706088537E-4	0.0011359210697130988
24	0.0	5.409147951014756E-5	0.0	3.786403565710329E-4	0.0011359210697130988
25	0.0	5.409147951014756E-5	0.0	5.409147951014756E-4	0.0011359210697130988
26	0.0	5.409147951014756E-5	0.0	7.572807131420658E-4	0.0012981955082435415
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	19.908892	1
GTATCAA	1685	0.0	19.862656	1
TCTAGCG	290	0.0	19.861298	28
CTAGCGG	295	0.0	19.525192	29
GTATTAC	170	0.0	16.955654	1
TAGCGGC	350	0.0	16.914087	30
AAGACGG	580	0.0	16.272589	5
CAAGACG	575	0.0	16.135883	4
GTCCTAC	580	0.0	15.737576	1
TACCGTC	365	0.0	14.902723	7
GCGCAAG	590	0.0	14.656582	1
GACGGAC	605	0.0	14.544102	7
AGCGGCG	410	0.0	14.438854	31
TAGGACG	290	0.0	14.341942	4
CGGTCCA	425	0.0	14.306099	10
ACGTTTT	180	1.1823431E-10	14.222054	29
CGCCGGT	395	0.0	14.175897	7
TCCTACA	555	0.0	14.129781	2
GCCGGTC	465	0.0	14.1062155	8
CGCGTAA	250	0.0	14.080214	10
>>END_MODULE