FastQCFastQC Report
Thu 2 Feb 2017
SRR4063072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848720
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22300.12062399930762907No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20480.1107793500367822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT18900.10223289627417889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGCG453.533936E-421.3368667
TCTAGCG3300.021.33225328
CTAGCGG3450.020.40476229
GGTATCA7000.019.6582721
GTATCAA15800.019.5454271
TAGCGGC3700.019.02606230
GGACCGT1208.185452E-1118.6692546
AAGACGG5500.018.0388645
CAAGACG5350.017.944474
CGCAAGA5350.017.9430142
GTATTAG4150.017.3503951
ACCGTTA652.0986154E-417.2336258
AGCGGCG4200.017.14198931
CGGTCCA4300.016.745610
AACGCTT2150.016.37126230
CGGACCA5650.016.1442689
GACGGAC5950.015.86817557
CGCCGGT4350.015.8187127
ACGGACC5800.015.7267448
CATCGTT4300.015.62711428
Produced by FastQC (version 0.11.5)