##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1848720
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.902764615517764	32.0	32.0	32.0	32.0	32.0
2	30.60388593188801	32.0	32.0	32.0	32.0	32.0
3	30.993524168073044	32.0	32.0	32.0	32.0	32.0
4	31.20925018391103	32.0	32.0	32.0	32.0	32.0
5	30.982025942273573	32.0	32.0	32.0	32.0	32.0
6	34.54040579427929	36.0	36.0	36.0	32.0	36.0
7	34.582752931758186	36.0	36.0	36.0	32.0	36.0
8	34.45956932364014	36.0	36.0	36.0	32.0	36.0
9	34.710982193084945	36.0	36.0	36.0	32.0	36.0
10	34.37698299363884	36.0	36.0	36.0	32.0	36.0
11	34.73708998658531	36.0	36.0	36.0	32.0	36.0
12	34.57074300056255	36.0	36.0	36.0	32.0	36.0
13	34.6829503656584	36.0	36.0	36.0	32.0	36.0
14	34.604909342680344	36.0	36.0	36.0	32.0	36.0
15	34.518769743390024	36.0	36.0	36.0	32.0	36.0
16	34.57264485698213	36.0	36.0	36.0	32.0	36.0
17	34.51677268596651	36.0	36.0	36.0	32.0	36.0
18	34.53620504998052	36.0	36.0	36.0	32.0	36.0
19	34.533399865853134	36.0	36.0	36.0	32.0	36.0
20	34.52666980397248	36.0	36.0	36.0	32.0	36.0
21	34.52454184516855	36.0	36.0	36.0	32.0	36.0
22	34.50392650049764	36.0	36.0	36.0	32.0	36.0
23	34.43429075252066	36.0	36.0	36.0	32.0	36.0
24	34.413307044874294	36.0	36.0	36.0	32.0	36.0
25	34.394773140334934	36.0	36.0	36.0	32.0	36.0
26	34.311330001298195	36.0	36.0	36.0	32.0	36.0
27	34.29823229044961	36.0	36.0	36.0	32.0	36.0
28	34.26416277208014	36.0	36.0	36.0	32.0	36.0
29	34.21631074473149	36.0	36.0	36.0	32.0	36.0
30	34.192249772815785	36.0	36.0	36.0	32.0	36.0
31	34.19167045307023	36.0	36.0	36.0	32.0	36.0
32	34.173987948418365	36.0	36.0	36.0	32.0	36.0
33	34.13188422259726	36.0	36.0	36.0	32.0	36.0
34	34.12932082738327	36.0	36.0	36.0	32.0	36.0
35	34.10757226621663	36.0	36.0	36.0	32.0	36.0
36	34.058802306460684	36.0	36.0	36.0	32.0	36.0
37	34.068385693885496	36.0	36.0	36.0	32.0	36.0
38	33.53000400276948	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	26.0
22	117.0
23	427.0
24	1088.0
25	3090.0
26	6657.0
27	13484.0
28	24720.0
29	41066.0
30	65588.0
31	101556.0
32	153488.0
33	252642.0
34	525499.0
35	659266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.690125759490385	18.970760702106666	12.401439353286364	26.937674185116585
2	15.934646674455841	19.994969492405556	36.402321606300575	27.668062226838032
3	18.572147215370634	23.027067376346878	28.650147128824266	29.75063827945822
4	12.565207419636115	15.979739451597757	35.55781418022021	35.89723894854591
5	14.904660908078412	36.007025412759226	33.213141870821765	15.875171808340596
6	35.87193936554953	35.62814880365745	15.335762404001043	13.16414942679197
7	30.989778851916682	29.9449996646321	20.018921240471766	19.046300242979452
8	28.04820723919976	34.18083664875303	18.19774596537539	19.573210146671823
9	26.666324074324503	14.085900522002543	18.001135964081897	41.24663943959106
10	15.225293449450966	27.015091686049658	31.833396440742145	25.926218423757234
11	38.50418554330922	20.703766312800052	21.461140036513626	19.330908107377105
12	24.36872333957551	23.30138680185253	28.69183659560105	23.63805326297091
13	29.591959820740353	19.154656071920225	24.915197853644287	26.338186253695135
14	23.01393559540417	19.304448107040606	24.660913854404047	33.02070244315118
15	24.986639404560993	27.67579730840798	21.53473754814142	25.802825738889613
16	25.858377688346536	25.925721580336663	23.315266779176945	24.90063395213986
17	24.27441689385088	26.168159591501144	24.705255528149205	24.852167986498767
18	25.139231468259123	25.059825176338222	25.549244882946038	24.251698472456617
19	25.470541780258777	25.172010904842267	25.00270457397551	24.35474274092345
20	25.62021290406335	24.568566359427063	24.613786836297546	25.19743390021204
21	26.381171837812108	24.41267471547882	24.482074083690335	24.724079363018735
22	25.585513853383805	24.58985091290289	24.907368241127095	24.917266992586214
23	24.48272318144446	24.360043705915444	25.445713791163616	25.711519321476484
24	24.904582630144102	25.03753948678004	24.961324592150245	25.096553290925616
25	24.947125521577657	24.73768308633334	25.165006236754333	25.15018515533467
26	24.743660478601413	25.452096585745814	25.294365831494225	24.509877104158555
27	25.32521167359669	24.87322302632255	24.831572564570386	24.96999273551037
28	24.65570773291791	24.810950279112035	25.48428101605435	25.049060971915704
29	24.619195984248563	25.05923017006361	25.264453243325114	25.057120602362716
30	24.49813925310485	25.100285603011812	25.562930027262105	24.83864511662123
31	25.19856982128175	24.763133411225063	24.861633995413044	25.176662772080142
32	25.000054091479512	24.885650612315548	24.636559349171318	25.477735947033626
33	24.509011640486392	24.834263706780906	25.140583755246876	25.51614089748583
34	25.20570989657709	24.839023756977802	25.225723743995847	24.72954260244926
35	25.42651131593751	24.730462157600936	25.3880522740058	24.454974252455752
36	24.54930979272145	25.189969276039637	25.10688476351205	25.153836167726862
37	25.47357090311134	24.853898913843093	24.772545328659827	24.899984854385735
38	24.73208490198624	24.65770911765979	25.133714137349084	25.47649184300489
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	17.5
2	32.0
3	32.0
4	94.0
5	156.0
6	156.0
7	242.0
8	328.0
9	372.0
10	416.0
11	416.0
12	682.0
13	948.0
14	1622.0
15	2296.0
16	2296.0
17	3658.0
18	5020.0
19	5020.0
20	6654.0
21	8288.0
22	10503.0
23	12718.0
24	12718.0
25	15886.0
26	19054.0
27	19054.0
28	23881.5
29	28709.0
30	35073.0
31	41437.0
32	41437.0
33	52196.0
34	62955.0
35	62955.0
36	71551.5
37	80148.0
38	90782.0
39	101416.0
40	101416.0
41	109095.5
42	116775.0
43	130465.0
44	144155.0
45	144155.0
46	148341.0
47	152527.0
48	152527.0
49	162081.0
50	171635.0
51	175567.5
52	179500.0
53	179500.0
54	173256.0
55	167012.0
56	167012.0
57	159723.5
58	152435.0
59	137570.5
60	122706.0
61	122706.0
62	111982.0
63	101258.0
64	85035.5
65	68813.0
66	68813.0
67	57335.0
68	45857.0
69	45857.0
70	36280.0
71	26703.0
72	20506.5
73	14310.0
74	14310.0
75	10843.0
76	7376.0
77	7376.0
78	6638.0
79	5900.0
80	4708.0
81	3516.0
82	3516.0
83	3070.0
84	2624.0
85	2624.0
86	1770.0
87	916.0
88	727.5
89	539.0
90	539.0
91	343.5
92	148.0
93	98.0
94	48.0
95	48.0
96	33.0
97	18.0
98	18.0
99	21.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006058245705136527
2	0.0
3	0.0
4	2.1636591804059024E-4
5	1.6227443853044268E-4
6	1.0818295902029512E-4
7	2.1636591804059024E-4
8	0.0018932017828551648
9	0.0037864035657103295
10	0.001081829590202951
11	0.005138690553464018
12	3.2454887706088537E-4
13	2.7045739755073776E-4
14	5.409147951014756E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0818295902029512E-4
23	0.0
24	0.0
25	1.0818295902029512E-4
26	0.0
27	5.409147951014756E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1848720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.51789644411109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05901805452308	40.92059773493154
2	14.384166100067302	15.683889557567248
3	4.733265896951613	7.741431000373518
4	2.0712462726837333	4.516799592176914
5	1.073576712473233	2.926457201771247
6	0.6577167395661769	2.151439985833633
7	0.4261138534006005	1.626158165316655
8	0.2884109933990086	1.2578848537176286
9	0.21191577163045855	1.0397881883360909
>10	0.9715718538313801	9.15575653921902
>50	0.060953391280575905	2.3189770587274077
>100	0.05266872680230018	6.129293820972498
>500	0.006483150748800914	2.4150812270222124
>1k	0.0028924826417727153	2.116445074034281
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2230	0.12062399930762907	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2048	0.1107793500367822	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1890	0.10223289627417889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.409147951014756E-5
2	0.0	0.0	0.0	0.0	5.409147951014756E-5
3	0.0	0.0	0.0	0.0	5.409147951014756E-5
4	0.0	0.0	0.0	0.0	5.409147951014756E-5
5	0.0	0.0	0.0	0.0	5.409147951014756E-5
6	0.0	0.0	0.0	0.0	5.409147951014756E-5
7	0.0	0.0	0.0	0.0	5.409147951014756E-5
8	0.0	0.0	0.0	0.0	5.409147951014756E-5
9	0.0	0.0	0.0	0.0	5.409147951014756E-5
10	0.0	0.0	0.0	0.0	5.409147951014756E-5
11	0.0	0.0	0.0	0.0	5.409147951014756E-5
12	0.0	5.409147951014756E-5	0.0	0.0	2.1636591804059024E-4
13	0.0	5.409147951014756E-5	0.0	0.0	2.704573975507378E-4
14	0.0	5.409147951014756E-5	0.0	0.0	2.704573975507378E-4
15	0.0	5.409147951014756E-5	0.0	0.0	2.704573975507378E-4
16	0.0	5.409147951014756E-5	0.0	0.0	2.704573975507378E-4
17	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	2.704573975507378E-4
18	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	3.786403565710329E-4
19	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	4.868233155913281E-4
20	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	5.950062746116232E-4
21	0.0	5.409147951014756E-5	0.0	5.409147951014756E-5	7.031892336319183E-4
22	0.0	5.409147951014756E-5	0.0	1.0818295902029512E-4	7.031892336319183E-4
23	0.0	5.409147951014756E-5	0.0	1.6227443853044268E-4	7.031892336319183E-4
24	0.0	5.409147951014756E-5	0.0	1.6227443853044268E-4	7.031892336319183E-4
25	0.0	5.409147951014756E-5	0.0	2.1636591804059024E-4	7.031892336319183E-4
26	0.0	5.409147951014756E-5	0.0	2.704573975507378E-4	8.654636721623609E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGCG	45	3.533936E-4	21.336866	7
TCTAGCG	330	0.0	21.332253	28
CTAGCGG	345	0.0	20.404762	29
GGTATCA	700	0.0	19.658272	1
GTATCAA	1580	0.0	19.545427	1
TAGCGGC	370	0.0	19.026062	30
GGACCGT	120	8.185452E-11	18.669254	6
AAGACGG	550	0.0	18.038864	5
CAAGACG	535	0.0	17.94447	4
CGCAAGA	535	0.0	17.943014	2
GTATTAG	415	0.0	17.350395	1
ACCGTTA	65	2.0986154E-4	17.233625	8
AGCGGCG	420	0.0	17.141989	31
CGGTCCA	430	0.0	16.7456	10
AACGCTT	215	0.0	16.371262	30
CGGACCA	565	0.0	16.144268	9
GACGGAC	595	0.0	15.8681755	7
CGCCGGT	435	0.0	15.818712	7
ACGGACC	580	0.0	15.726744	8
CATCGTT	430	0.0	15.627114	28
>>END_MODULE