FastQCFastQC Report
Thu 2 Feb 2017
SRR4063071_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063071_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510859
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT98510.6520131924951302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT66120.43763183725284754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT35770.2367527347025765No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA32540.21537416794022474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.1951207889022073No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA28610.1893624752541435No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC25140.1663954081750845No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA17350.114835335395295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG308.430154E-426.660135
CGTTTGA507.19642E-419.20037710
GACGACG450.00885896517.77518518
TATCCGC450.00886403417.773426
TACGACG2850.017.3992425
CTACGAC2950.016.8088824
GTCCTAA7350.016.5587961
CGACGGT3000.016.5309227
GATATAC5550.016.446911
ACGACGG3050.016.258316
TAACCCG600.002442601815.99978528
GTCCTAC10550.015.786411
CGAATGT2450.015.02020723
GACGGTA3350.014.8038118
TAGGACG11300.014.7215664
TCGTTCT2950.014.64435630
ACGGTAT3400.014.5885229
CGAAAAC2250.014.22203123
TTTCGTT3150.014.22203128
CTTTCGT3150.014.221560527
Produced by FastQC (version 0.11.5)