##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063071_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1510859
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.087722944364764	32.0	32.0	32.0	32.0	32.0
2	30.738013937766528	32.0	32.0	32.0	32.0	32.0
3	30.814638559918563	32.0	32.0	32.0	32.0	32.0
4	30.855144656119467	32.0	32.0	32.0	32.0	32.0
5	30.81669699157896	32.0	32.0	32.0	32.0	32.0
6	34.43678331333367	36.0	36.0	36.0	32.0	36.0
7	34.320529579530586	36.0	36.0	36.0	32.0	36.0
8	34.286817631559266	36.0	36.0	36.0	32.0	36.0
9	34.38622134825288	36.0	36.0	36.0	32.0	36.0
10	34.153813161916496	36.0	36.0	36.0	32.0	36.0
11	34.455125196990586	36.0	36.0	36.0	32.0	36.0
12	34.28745104606055	36.0	36.0	36.0	32.0	36.0
13	34.35499209390155	36.0	36.0	36.0	32.0	36.0
14	34.26652123063767	36.0	36.0	36.0	32.0	36.0
15	34.23462149677766	36.0	36.0	36.0	32.0	36.0
16	34.244914978829925	36.0	36.0	36.0	32.0	36.0
17	34.1843044254957	36.0	36.0	36.0	32.0	36.0
18	34.208746812243895	36.0	36.0	36.0	32.0	36.0
19	34.17876453064118	36.0	36.0	36.0	32.0	36.0
20	34.11574806120227	36.0	36.0	36.0	32.0	36.0
21	34.0866116560182	36.0	36.0	36.0	32.0	36.0
22	34.069059389393715	36.0	36.0	36.0	32.0	36.0
23	34.04964195864736	36.0	36.0	36.0	32.0	36.0
24	34.0323881977074	36.0	36.0	36.0	32.0	36.0
25	34.015120537389656	36.0	36.0	36.0	32.0	36.0
26	33.9932468880286	36.0	36.0	36.0	32.0	36.0
27	33.990925030065675	36.0	36.0	36.0	32.0	36.0
28	33.95875392740157	36.0	36.0	36.0	32.0	36.0
29	33.90863541865919	36.0	36.0	36.0	32.0	36.0
30	33.8690076307584	36.0	36.0	36.0	32.0	36.0
31	33.8799563691913	36.0	36.0	36.0	32.0	36.0
32	33.82183380447812	36.0	36.0	36.0	32.0	36.0
33	33.77619420475372	36.0	36.0	36.0	32.0	36.0
34	33.755891185080806	36.0	36.0	36.0	32.0	36.0
35	33.69501058669274	36.0	36.0	36.0	27.0	36.0
36	33.643992589646025	36.0	36.0	36.0	27.0	36.0
37	33.62976161243372	36.0	36.0	36.0	27.0	36.0
38	32.98735024247795	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	4.0
9	4.0
10	17.0
11	22.0
12	3.0
13	10.0
14	370.0
15	767.0
16	902.0
17	1094.0
18	1414.0
19	1734.0
20	2195.0
21	2835.0
22	3897.0
23	5512.0
24	7762.0
25	11245.0
26	15717.0
27	22425.0
28	31290.0
29	41747.0
30	56111.0
31	74470.0
32	100438.0
33	148307.0
34	322744.0
35	657820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.612895743940626	17.753052467652957	11.945196401650072	24.68885538675635
2	14.944982897181097	20.942658370778517	40.60027086361785	23.512087868422533
3	18.64842447259004	26.61327638605436	29.174249008861825	25.564050132493776
4	11.680629384654182	16.560001959215228	37.63089841270472	34.12847024342587
5	12.814152005522708	38.52876487653001	34.439816448059474	14.217266669887804
6	31.040391725474464	37.3514581819289	18.435930937072676	13.172219155523962
7	27.649105575040423	32.08267614648356	22.328688514282273	17.939529764193747
8	26.707478022363514	34.670524087701395	20.53004459729398	18.091953292641115
9	27.85195941903133	14.376552100266341	19.377889090687663	38.393599390014664
10	15.093261885835352	28.314270150702946	33.267432370985134	23.325035592476567
11	35.61808945443715	22.011880925984148	23.956580014230635	18.41344960534807
12	24.473001416449343	24.642313445678507	30.923935346368197	19.96074979150395
13	29.79566222438761	21.1988195700354	25.910940822276178	23.09457738330081
14	22.77741150553777	20.99818893822532	27.271307115301617	28.95309244093529
15	23.50119364290812	29.180431406247724	24.73243994380676	22.585935007037396
16	22.90566732155245	27.05263004601852	27.06970826691803	22.971994365510998
17	21.881748405539202	26.951319460167277	27.687479063806236	23.47945307048728
18	22.541872299415402	26.009542065576547	29.86945850631195	21.579127128696094
19	24.00620618487097	25.937298734205704	27.79677127468392	22.25972380623941
20	24.532867872548998	25.27209796916918	28.272837331649413	21.922196826632405
21	25.226935454618864	25.188610931292537	26.507265773619228	23.077187840469374
22	24.071426396550606	26.28289904802755	27.20415844231836	22.44151611310349
23	22.52828430191457	26.332599031462895	27.69127813536749	23.447838531255048
24	23.16534227967558	26.551598134825333	27.88395086572808	22.39910871977101
25	23.371667039588402	26.097283585132736	27.88187957849694	22.64916979678193
26	21.94309470534497	27.1712852017106	28.56982086350938	22.31579922943505
27	22.856373127234843	26.801872467290732	27.878700022638004	22.46305438283642
28	22.173718786715426	27.150105078519303	27.7041915573133	22.97198457745197
29	22.390850766937362	26.72550976908473	27.72121767358362	23.162421790394287
30	22.318123811894033	27.01077833847848	28.037977029510035	22.633120820117448
31	23.041577143492574	26.63977992854032	27.268924305425934	23.049718622541175
32	22.235166406312054	27.504368662130318	27.355039847493977	22.905425084063648
33	22.050687675327755	27.00600102466205	28.036686608302407	22.906624691707783
34	22.52557287001972	27.352991991039815	28.105300714974618	22.01613442396585
35	22.97865974147022	27.242938159787478	27.853019192794477	21.925382905947828
36	22.18520293600525	27.79480532548139	27.67386192725655	22.346129811256805
37	22.70129189285438	27.859397681516672	26.9207779820206	22.51853244360835
38	22.363767662199006	28.283479493745954	27.01744724424355	22.335305599811488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1091.0
1	958.5
2	826.0
3	826.0
4	2073.0
5	3320.0
6	3320.0
7	3778.5
8	4237.0
9	4054.0
10	3871.0
11	3871.0
12	4869.5
13	5868.0
14	6890.5
15	7913.0
16	7913.0
17	11530.5
18	15148.0
19	15148.0
20	17614.0
21	20080.0
22	19741.5
23	19403.0
24	19403.0
25	23075.5
26	26748.0
27	26748.0
28	34177.5
29	41607.0
30	51233.0
31	60859.0
32	60859.0
33	69812.0
34	78765.0
35	78765.0
36	83465.0
37	88165.0
38	95981.0
39	103797.0
40	103797.0
41	106911.5
42	110026.0
43	115751.5
44	121477.0
45	121477.0
46	126651.0
47	131825.0
48	131825.0
49	130417.5
50	129010.0
51	123604.5
52	118199.0
53	118199.0
54	109680.0
55	101161.0
56	101161.0
57	95906.0
58	90651.0
59	80239.0
60	69827.0
61	69827.0
62	64283.0
63	58739.0
64	48157.0
65	37575.0
66	37575.0
67	31008.0
68	24441.0
69	24441.0
70	19472.5
71	14504.0
72	11328.0
73	8152.0
74	8152.0
75	6178.5
76	4205.0
77	4205.0
78	3787.0
79	3369.0
80	2649.0
81	1929.0
82	1929.0
83	1876.0
84	1823.0
85	1823.0
86	1222.5
87	622.0
88	521.0
89	420.0
90	420.0
91	323.5
92	227.0
93	201.0
94	175.0
95	175.0
96	167.5
97	160.0
98	160.0
99	402.0
100	644.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1213878992017124
2	0.05784788653342238
3	0.014693627929542069
4	0.0033093756598067724
5	3.9712507917681264E-4
6	3.309375659806772E-4
7	0.0
8	3.309375659806772E-4
9	1.9856253958840632E-4
10	3.9712507917681264E-4
11	0.002250375448668605
12	0.0025813130146492822
13	0.014759815442738205
14	0.009464814387047368
15	0.02362894221102035
16	0.010788564650970077
17	0.020981441683174937
18	0.004964063489710158
19	0.007148251425182628
20	0.006486376293221273
21	0.005096438516102429
22	0.006684938832809679
23	0.008405814175909201
24	0.014759815442738205
25	0.01753969099697589
26	0.018466316181721787
27	0.00827343914951693
28	0.005559751108475377
29	0.011582814809323704
30	0.003375563173002908
31	0.005096438516102429
32	0.006552563806417409
33	0.007743939043947846
34	0.011649002322519838
35	0.015885003167072507
36	0.01555406560109183
37	0.008604376715497608
38	0.005030251002906294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1510859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.6014232457414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09154715380937	43.06889865876053
2	14.498380755555552	16.412579710462232
3	4.719719281199416	8.014284859087635
4	1.924224541770567	4.356553908343947
5	0.9274589184677687	2.624774739361587
6	0.500132353752617	1.6984921820184442
7	0.29240830824539	1.1585508490897973
8	0.18541849047613362	0.8395960365620887
9	0.1378071466809134	0.7020072572016912
>10	0.5902889078346172	6.128625190993409
>50	0.06233380777215375	2.465837227903493
>100	0.06184683235445713	7.054814183489929
>500	0.0064422585341419435	2.5538129809260197
>1k	0.0017569796002205298	1.8297022494183877
>5k	2.3426394669607065E-4	1.0914699663807128
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9851	0.6520131924951302	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6612	0.43763183725284754	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3577	0.2367527347025765	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3254	0.21537416794022474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2948	0.1951207889022073	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2861	0.1893624752541435	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2514	0.1663954081750845	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1735	0.114835335395295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.618751319613544E-5	0.0
8	0.0	0.0	0.0	6.618751319613544E-5	0.0
9	0.0	0.0	0.0	6.618751319613544E-5	0.0
10	0.0	0.0	0.0	6.618751319613544E-5	0.0
11	0.0	0.0	0.0	6.618751319613544E-5	0.0
12	0.0	0.0	0.0	6.618751319613544E-5	0.0
13	0.0	0.0	0.0	6.618751319613544E-5	0.0
14	0.0	0.0	0.0	6.618751319613544E-5	0.0
15	0.0	0.0	0.0	6.618751319613544E-5	0.0
16	0.0	0.0	0.0	1.323750263922709E-4	0.0
17	0.0	0.0	0.0	1.9856253958840632E-4	0.0
18	0.0	0.0	0.0	1.9856253958840632E-4	0.0
19	0.0	0.0	0.0	2.647500527845418E-4	0.0
20	0.0	0.0	0.0	4.633125923729481E-4	0.0
21	0.0	0.0	0.0	5.295001055690836E-4	0.0
22	0.0	0.0	0.0	0.001059000211138167	0.0
23	0.0	0.0	0.0	0.0021841879354724696	0.0
24	0.0	0.0	0.0	0.004169813331356533	0.0
25	0.0	0.0	0.0	0.004765500950121752	0.0
26	0.0	0.0	0.0	0.006155438727240596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	30	8.430154E-4	26.66013	5
CGTTTGA	50	7.19642E-4	19.200377	10
GACGACG	45	0.008858965	17.775185	18
TATCCGC	45	0.008864034	17.77342	6
TACGACG	285	0.0	17.399242	5
CTACGAC	295	0.0	16.808882	4
GTCCTAA	735	0.0	16.558796	1
CGACGGT	300	0.0	16.530922	7
GATATAC	555	0.0	16.44691	1
ACGACGG	305	0.0	16.25831	6
TAACCCG	60	0.0024426018	15.999785	28
GTCCTAC	1055	0.0	15.78641	1
CGAATGT	245	0.0	15.020207	23
GACGGTA	335	0.0	14.803811	8
TAGGACG	1130	0.0	14.721566	4
TCGTTCT	295	0.0	14.644356	30
ACGGTAT	340	0.0	14.588522	9
CGAAAAC	225	0.0	14.222031	23
TTTCGTT	315	0.0	14.222031	28
CTTTCGT	315	0.0	14.2215605	27
>>END_MODULE