FastQCFastQC Report
Thu 2 Feb 2017
SRR4063071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510859
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT114430.757383713503378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT83800.554651360583615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT48210.31909000111856894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36820.24370242358817068No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC30760.2035927905913126No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA30750.20352660307811649No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA26760.17711778531285846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.10451008333669787No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.10153164524287178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA400.00448320920.0007049
TGCACCG852.3946814E-718.825445
AACGCTT2150.017.11574730
GATATAC5700.016.8432541
ATACACT7250.016.5523054
GTAAACG2150.016.37158427
CTAGCGG2150.016.37158429
CGCTTCG2350.016.33991832
TCTAGCG2100.015.99950428
ACGGCTA1850.015.56708424
AACGAAT3500.015.54237531
AAACGCT2400.015.33285829
ACTGTTC7000.015.31533058
TAGGACG13200.015.0308314
CCAACGA3200.014.99953629
TGTAGGA11700.014.9065072
CTATTAC1205.415277E-714.6676681
ATAGGGG1205.417314E-714.6671833
TATACAC7200.014.6671833
TAAACGC2400.014.66621228
Produced by FastQC (version 0.11.5)