##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063071_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1510859
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05035810754015	32.0	32.0	32.0	32.0	32.0
2	31.25474647204008	32.0	32.0	32.0	32.0	32.0
3	31.355315088965945	32.0	32.0	32.0	32.0	32.0
4	31.461903460217002	32.0	32.0	32.0	32.0	32.0
5	31.37034362571226	32.0	32.0	32.0	32.0	32.0
6	34.87783969251929	36.0	36.0	36.0	36.0	36.0
7	34.91681090028917	36.0	36.0	36.0	36.0	36.0
8	34.86038405966407	36.0	36.0	36.0	32.0	36.0
9	34.956467148820636	36.0	36.0	36.0	32.0	36.0
10	34.8228047752967	36.0	36.0	36.0	32.0	36.0
11	35.00312206499746	36.0	36.0	36.0	36.0	36.0
12	34.90363892328801	36.0	36.0	36.0	32.0	36.0
13	34.951843289148755	36.0	36.0	36.0	36.0	36.0
14	34.893262706844254	36.0	36.0	36.0	32.0	36.0
15	34.86204801374582	36.0	36.0	36.0	32.0	36.0
16	34.87884177146908	36.0	36.0	36.0	32.0	36.0
17	34.86045686592859	36.0	36.0	36.0	32.0	36.0
18	34.842567704861935	36.0	36.0	36.0	32.0	36.0
19	34.825991704057095	36.0	36.0	36.0	32.0	36.0
20	34.810943311056825	36.0	36.0	36.0	32.0	36.0
21	34.785694760397895	36.0	36.0	36.0	32.0	36.0
22	34.77287490096693	36.0	36.0	36.0	32.0	36.0
23	34.724176114382615	36.0	36.0	36.0	32.0	36.0
24	34.709814747769315	36.0	36.0	36.0	32.0	36.0
25	34.68925822992086	36.0	36.0	36.0	32.0	36.0
26	34.62176814646502	36.0	36.0	36.0	32.0	36.0
27	34.597427688487144	36.0	36.0	36.0	32.0	36.0
28	34.56246810589208	36.0	36.0	36.0	32.0	36.0
29	34.52322420556783	36.0	36.0	36.0	32.0	36.0
30	34.490747316592746	36.0	36.0	36.0	32.0	36.0
31	34.47157941277114	36.0	36.0	36.0	32.0	36.0
32	34.42947555000169	36.0	36.0	36.0	32.0	36.0
33	34.38230039997114	36.0	36.0	36.0	32.0	36.0
34	34.33953267644433	36.0	36.0	36.0	32.0	36.0
35	34.28989667467315	36.0	36.0	36.0	32.0	36.0
36	34.24180681321023	36.0	36.0	36.0	32.0	36.0
37	34.21161405531555	36.0	36.0	36.0	32.0	36.0
38	33.79907191868996	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	11.0
21	37.0
22	105.0
23	349.0
24	975.0
25	2472.0
26	5332.0
27	10487.0
28	18495.0
29	29592.0
30	44891.0
31	64634.0
32	92465.0
33	145811.0
34	345192.0
35	750007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.946503866353	18.983786603262274	12.650672590529322	25.419036939855406
2	14.148904695937874	21.989212759099296	39.55438594865569	24.307496596307136
3	17.684376900822645	27.18294691960004	29.64340153515318	25.489274644424132
4	11.350959950597641	17.398314468788946	37.563862676795125	33.686862903818295
5	12.444708606163777	38.87655962601407	34.09312185981617	14.585609908005976
6	31.18293946143078	37.777615985650506	17.937393065515884	13.102051487402827
7	27.355738778028044	32.10530739919768	22.38576486263058	18.15318896014371
8	26.581684456052518	34.89170824436434	20.411351141693352	18.11525615788979
9	27.612390786338363	14.150251522372253	19.227230606301298	39.010127084988085
10	14.8715355502811	28.427980085329672	33.414987364695016	23.28549699969421
11	35.60592220748377	21.846082971270622	23.939427636438733	18.60856718480687
12	24.174306947338323	24.685210728780845	31.04367601856436	20.09680630531647
13	29.858729278330593	21.077030402592435	25.911501941281045	23.152738377795927
14	22.642086389265977	20.914989419926016	27.451138723070784	28.99178546773723
15	23.528668128528206	29.151628312105892	24.749827746996907	22.569875812368988
16	22.91345519337013	26.84267691425871	27.150978350726305	23.092889541644855
17	21.813617286589945	26.930441556756783	27.76016822218354	23.495772934469727
18	22.50441636181801	25.676254369203217	30.079511059602517	21.739818209376256
19	23.946973211927784	25.724438878810002	28.10057060255126	22.22801730671095
20	24.452381062693473	25.141128325012456	28.46513142523558	21.941359187058488
21	25.02068361156376	25.08660641833978	26.850901937839293	23.04180803225717
22	23.96540506480759	26.11464883837451	27.450513185562897	22.469432911255
23	22.42831556638678	25.959951233008	28.08417468749545	23.52755851310977
24	23.071312412342913	26.321648810378733	28.12618517015817	22.48085360712019
25	23.296945644828536	25.86866147006438	28.18184886875612	22.652544016350966
26	22.016378783696936	26.81392084095318	28.755070797567207	22.414629577782673
27	23.00700860504998	26.50813412520179	28.073868010010212	22.410989259738017
28	22.09815740582013	26.684554945233142	28.090112975466276	23.12717467348045
29	22.447693663008923	26.207012037523025	28.11407285524328	23.231221444224776
30	22.236356933373663	26.536427290700193	28.54475500361053	22.682460772315615
31	22.976929018525222	26.042800817283414	27.774067599954726	23.206202564236637
32	22.227752556658164	26.94440712204117	27.82847373580195	22.999366585498713
33	22.066585962025577	26.456605149785652	28.488429429880618	22.988379458308156
34	22.61494950885556	26.820570284851204	28.508484246379044	22.055995959914195
35	23.115128546078754	26.70394788659961	28.163183990034806	22.01773957728683
36	22.266207501825118	27.26985112442657	28.010621772117716	22.453319601630596
37	22.765327538837177	27.319822696889652	27.28613325267282	22.628716511600356
38	22.48628098320227	27.508655672038223	27.550022867785813	22.455040476973696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	647.0
1	565.0
2	483.0
3	483.0
4	1696.0
5	2909.0
6	2909.0
7	3501.0
8	4093.0
9	4073.5
10	4054.0
11	4054.0
12	5199.0
13	6344.0
14	7418.0
15	8492.0
16	8492.0
17	12574.5
18	16657.0
19	16657.0
20	18836.0
21	21015.0
22	20582.0
23	20149.0
24	20149.0
25	22982.5
26	25816.0
27	25816.0
28	33205.5
29	40595.0
30	50372.0
31	60149.0
32	60149.0
33	69168.0
34	78187.0
35	78187.0
36	83023.5
37	87860.0
38	95754.0
39	103648.0
40	103648.0
41	106624.0
42	109600.0
43	114940.0
44	120280.0
45	120280.0
46	126732.5
47	133185.0
48	133185.0
49	132449.0
50	131713.0
51	124514.0
52	117315.0
53	117315.0
54	109930.0
55	102545.0
56	102545.0
57	96820.5
58	91096.0
59	80191.5
60	69287.0
61	69287.0
62	64068.0
63	58849.0
64	48197.5
65	37546.0
66	37546.0
67	30906.5
68	24267.0
69	24267.0
70	19366.5
71	14466.0
72	11273.0
73	8080.0
74	8080.0
75	6102.0
76	4124.0
77	4124.0
78	3712.0
79	3300.0
80	2561.5
81	1823.0
82	1823.0
83	1651.0
84	1479.0
85	1479.0
86	953.5
87	428.0
88	348.5
89	269.0
90	269.0
91	162.0
92	55.0
93	41.5
94	28.0
95	28.0
96	21.5
97	15.0
98	15.0
99	13.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008802939255086014
2	0.0
3	0.0
4	0.0
5	0.0
6	2.647500527845418E-4
7	3.9712507917681264E-4
8	0.0021841879354724696
9	0.0039050632785719913
10	8.604376715497607E-4
11	0.0046993134369256164
12	4.6331259237294807E-4
13	6.618751319613544E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	6.618751319613544E-5
22	1.323750263922709E-4
23	6.618751319613544E-5
24	0.0
25	0.0
26	1.323750263922709E-4
27	1.323750263922709E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1510859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.410693137318354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27594012085883	40.414053854018825
2	15.49610374672858	16.86307491574596
3	5.142816281298692	8.394725956300435
4	2.1098016209844555	4.591830743200083
5	1.0086235399870447	2.7439952962655423
6	0.5378901315346488	1.756018493311415
7	0.3182370950513303	1.2120850646624681
8	0.1978562249049244	0.861239547088807
9	0.13631456370618214	0.6675272906368059
>10	0.6331809653885241	6.2456932709003885
>50	0.06745885524570285	2.573186562304066
>100	0.06673264175914036	7.5502331776206795
>500	0.006599830781143888	2.54935370746356
>1k	0.0021999435937146293	2.258747600889693
>5k	1.2221908853970163E-4	0.5572721219883303
>10k+	1.2221908853970163E-4	0.7609623976029194
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11443	0.757383713503378	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	8380	0.554651360583615	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4821	0.31909000111856894	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3682	0.24370242358817068	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3076	0.2035927905913126	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3075	0.20352660307811649	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2676	0.17711778531285846	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1579	0.10451008333669787	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1534	0.10153164524287178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.618751319613544E-5	0.0
8	0.0	0.0	0.0	6.618751319613544E-5	0.0
9	0.0	0.0	0.0	6.618751319613544E-5	0.0
10	0.0	0.0	0.0	6.618751319613544E-5	0.0
11	0.0	0.0	0.0	6.618751319613544E-5	0.0
12	0.0	0.0	0.0	6.618751319613544E-5	0.0
13	0.0	0.0	0.0	6.618751319613544E-5	0.0
14	0.0	0.0	0.0	6.618751319613544E-5	0.0
15	0.0	0.0	0.0	6.618751319613544E-5	0.0
16	0.0	0.0	0.0	1.323750263922709E-4	0.0
17	0.0	0.0	0.0	1.323750263922709E-4	0.0
18	0.0	0.0	0.0	1.323750263922709E-4	0.0
19	0.0	0.0	0.0	1.9856253958840632E-4	0.0
20	0.0	0.0	0.0	3.9712507917681264E-4	0.0
21	0.0	0.0	0.0	5.295001055690836E-4	0.0
22	0.0	0.0	0.0	0.001059000211138167	0.0
23	0.0	0.0	0.0	0.0021841879354724696	0.0
24	0.0	0.0	0.0	0.004236000844552668	0.0
25	0.0	0.0	0.0	0.005030251002906294	0.0
26	0.0	0.0	0.0	0.006618751319613544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	40	0.004483209	20.000704	9
TGCACCG	85	2.3946814E-7	18.82544	5
AACGCTT	215	0.0	17.115747	30
GATATAC	570	0.0	16.843254	1
ATACACT	725	0.0	16.552305	4
GTAAACG	215	0.0	16.371584	27
CTAGCGG	215	0.0	16.371584	29
CGCTTCG	235	0.0	16.339918	32
TCTAGCG	210	0.0	15.999504	28
ACGGCTA	185	0.0	15.567084	24
AACGAAT	350	0.0	15.542375	31
AAACGCT	240	0.0	15.332858	29
ACTGTTC	700	0.0	15.3153305	8
TAGGACG	1320	0.0	15.030831	4
CCAACGA	320	0.0	14.999536	29
TGTAGGA	1170	0.0	14.906507	2
CTATTAC	120	5.415277E-7	14.667668	1
ATAGGGG	120	5.417314E-7	14.667183	3
TATACAC	720	0.0	14.667183	3
TAAACGC	240	0.0	14.666212	28
>>END_MODULE