FastQCFastQC Report
Thu 2 Feb 2017
SRR4063070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426206
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28310.6642327888391998No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24580.5767164235135123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20710.4859152616340455No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18980.4453245613623459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11190.2625490959770627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.24612511320816696No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9630.2259470772349521No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9030.2118693777187557No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6370.14945824319695172No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5190.1217721008150988No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5070.11895656091185952No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4970.11661027765916011No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4830.11332548110538096No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4570.10722514464836252No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC4570.10722514464836252No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA4440.10417497641985331No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG4430.10394034809458337No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.10300183479350362No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4280.10042092321553428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAA401.590466E-424.0017611
GTACAGG555.7875037E-520.3794821
GTCTAGA400.00445881120.0155621
GTCTTAA400.00445881120.0155621
CACGTGC400.004483114519.9967758
TATACTG400.004486160419.9944275
CGAATGT601.13540904E-418.66803623
CGCTGAG1159.349606E-1018.08828412
CTATAGA450.0088018617.791611
AGCATAA450.00883765617.77908122
TCGCTCG450.00884961517.77491219
GCTCTTA1550.017.562041
ACACGCT1201.6480044E-917.3346049
CACGCTG1201.6480044E-917.33460410
ACGCTGA1201.6480044E-917.33460411
TAGCGGC1404.0017767E-1117.14411530
CTAGCGG1404.0017767E-1117.14411529
GCGAGAA753.2239506E-517.06791920
AGGGGCG753.2239506E-517.06791932
CATCGTT957.6680226E-716.84136428
Produced by FastQC (version 0.11.5)