##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063070_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426206
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.045004059070028	32.0	32.0	32.0	32.0	32.0
2	30.73958367549964	32.0	32.0	32.0	32.0	32.0
3	30.822358202371625	32.0	32.0	32.0	32.0	32.0
4	30.837759205642342	32.0	32.0	32.0	32.0	32.0
5	30.836534445784434	32.0	32.0	32.0	32.0	32.0
6	34.43380665687484	36.0	36.0	36.0	32.0	36.0
7	34.29893994922643	36.0	36.0	36.0	32.0	36.0
8	34.276267814155595	36.0	36.0	36.0	32.0	36.0
9	34.344617391589985	36.0	36.0	36.0	32.0	36.0
10	34.12843554525276	36.0	36.0	36.0	32.0	36.0
11	34.42698131889274	36.0	36.0	36.0	32.0	36.0
12	34.258673036043604	36.0	36.0	36.0	32.0	36.0
13	34.32139857252127	36.0	36.0	36.0	32.0	36.0
14	34.228427098633055	36.0	36.0	36.0	32.0	36.0
15	34.21010497271273	36.0	36.0	36.0	32.0	36.0
16	34.20667705288053	36.0	36.0	36.0	32.0	36.0
17	34.13019525769229	36.0	36.0	36.0	32.0	36.0
18	34.15911554506506	36.0	36.0	36.0	32.0	36.0
19	34.13821250756676	36.0	36.0	36.0	32.0	36.0
20	34.07614862296636	36.0	36.0	36.0	32.0	36.0
21	34.04461457605008	36.0	36.0	36.0	32.0	36.0
22	34.016257396657956	36.0	36.0	36.0	32.0	36.0
23	33.97097412988086	36.0	36.0	36.0	32.0	36.0
24	33.9879213338151	36.0	36.0	36.0	32.0	36.0
25	33.94344284219368	36.0	36.0	36.0	32.0	36.0
26	33.90527585252202	36.0	36.0	36.0	32.0	36.0
27	33.89196773391271	36.0	36.0	36.0	32.0	36.0
28	33.86337592619532	36.0	36.0	36.0	32.0	36.0
29	33.8430688446432	36.0	36.0	36.0	32.0	36.0
30	33.76338906538153	36.0	36.0	36.0	32.0	36.0
31	33.79211226496108	36.0	36.0	36.0	32.0	36.0
32	33.74048230198542	36.0	36.0	36.0	27.0	36.0
33	33.64150903553681	36.0	36.0	36.0	27.0	36.0
34	33.63931056812903	36.0	36.0	36.0	27.0	36.0
35	33.556073823456266	36.0	36.0	36.0	27.0	36.0
36	33.524497543441434	36.0	36.0	36.0	21.0	36.0
37	33.476520743490234	36.0	36.0	36.0	21.0	36.0
38	32.82204849298227	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	5.0
12	0.0
13	1.0
14	148.0
15	237.0
16	296.0
17	374.0
18	429.0
19	596.0
20	687.0
21	877.0
22	1214.0
23	1738.0
24	2363.0
25	3337.0
26	4710.0
27	6514.0
28	9336.0
29	12213.0
30	16200.0
31	21658.0
32	28477.0
33	41159.0
34	87433.0
35	186198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.72193341210685	17.08884101923217	11.706793393360943	24.48243217530004
2	15.540781640280205	20.950476083159614	38.256615396172556	25.252126880387628
3	19.124731611737513	26.349802301979324	28.37300982037052	26.152456265912637
4	12.024364548455747	15.90922422470571	36.21622128941583	35.85018993742272
5	13.599324268837766	37.92869628465175	33.391912342652006	15.080067103858472
6	32.54380531388725	35.72373792831602	17.901521337200027	13.83093542059671
7	28.57726076122814	31.45638494061557	21.63179307658738	18.33456122156891
8	28.308142072143518	32.363223417783885	20.522470354711103	18.80616415536149
9	27.989751434752208	14.519035396029151	18.881010591122603	38.610202578096036
10	15.864666064452551	27.38001665865018	31.79268192536455	24.962635351532715
11	36.36346572125097	22.067259976020477	22.243704202516675	19.325570100211877
12	24.47547853345159	24.613911843790916	29.46118940023182	21.44942022252568
13	30.278583429542234	20.037732661278966	25.660321572381946	24.023362336796854
14	24.077602470364077	20.175891424119836	26.060839285546685	29.685666819969402
15	25.110533282017105	27.003914427057417	24.81061494991974	23.074937341005736
16	24.623857258170485	26.091822943064706	25.664761314786656	23.61955848397815
17	22.865322788820315	25.82193227418863	27.157909558116067	24.154835378874992
18	24.13272045970215	24.51048713129621	28.84612226031174	22.5106701486899
19	24.518575353779948	26.216672180729873	26.924349399201752	22.340403066288424
20	24.660944568543165	24.06190753289659	27.998235503913804	23.278912394646444
21	26.06450250478079	24.726820512218872	25.42111993617795	23.78755704682239
22	24.444308873459867	25.202906746194692	26.373770768757876	23.979013611587565
23	23.132913471556087	25.057019231671717	26.561341430220665	25.248725866551535
24	23.124413365871973	26.78993805143608	26.58156560916088	23.50408297353107
25	24.48914461642434	24.906135896449122	26.334747552493496	24.269971934633038
26	22.734579417320802	25.976040456662798	27.593133631360956	23.696246494655448
27	24.3307295394124	25.862501847841134	26.70934104225279	23.09742757049367
28	23.240376831657613	25.221441392822364	27.422832807348925	24.115348968171098
29	22.676919250344348	26.0072881884886	27.783109517585746	23.5326830435813
30	23.59934677635068	25.94006485309507	27.664162329831015	22.79642604072324
31	23.09583866161409	25.97862430634584	26.62505719347232	24.30047983856776
32	22.77647290705123	26.617329256818113	26.497427149497515	24.108770686633143
33	22.476236510949622	25.894518500442302	26.98373218764885	24.64551280095922
34	22.95728609609983	26.727457463259192	27.382139613901792	22.933116826739187
35	24.29349496769894	26.139336895484423	26.95173261808719	22.615435518729452
36	23.089666694825368	27.402641286289526	26.208008334975926	23.29968368390918
37	24.032550237932103	27.064424692377724	26.484611846859952	22.418413222830218
38	23.279687085715896	27.094913735050575	26.099810646500245	23.525588532733284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	565.0
1	439.5
2	314.0
3	314.0
4	720.5
5	1127.0
6	1127.0
7	1258.5
8	1390.0
9	1265.0
10	1140.0
11	1140.0
12	1421.0
13	1702.0
14	2087.0
15	2472.0
16	2472.0
17	3325.0
18	4178.0
19	4178.0
20	4691.0
21	5204.0
22	4895.0
23	4586.0
24	4586.0
25	5149.5
26	5713.0
27	5713.0
28	7253.0
29	8793.0
30	10630.0
31	12467.0
32	12467.0
33	14251.0
34	16035.0
35	16035.0
36	17400.5
37	18766.0
38	21293.0
39	23820.0
40	23820.0
41	25228.5
42	26637.0
43	30198.5
44	33760.0
45	33760.0
46	38032.0
47	42304.0
48	42304.0
49	41481.5
50	40659.0
51	38090.5
52	35522.0
53	35522.0
54	33323.0
55	31124.0
56	31124.0
57	30170.0
58	29216.0
59	26056.0
60	22896.0
61	22896.0
62	21683.5
63	20471.0
64	16925.5
65	13380.0
66	13380.0
67	10920.0
68	8460.0
69	8460.0
70	6839.5
71	5219.0
72	4045.0
73	2871.0
74	2871.0
75	2194.0
76	1517.0
77	1517.0
78	1461.5
79	1406.0
80	1087.0
81	768.0
82	768.0
83	787.5
84	807.0
85	807.0
86	541.5
87	276.0
88	238.0
89	200.0
90	200.0
91	144.0
92	88.0
93	82.0
94	76.0
95	76.0
96	69.5
97	63.0
98	63.0
99	138.5
100	214.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12036433086347917
2	0.053964514812086174
3	0.011966044588766935
4	0.0030501682285092183
5	2.346283252699399E-4
6	4.692566505398798E-4
7	0.0
8	0.0
9	0.0
10	2.346283252699399E-4
11	0.002111654927429459
12	0.0018770266021595193
13	0.011731416263496995
14	0.007977363059177957
15	0.02111654927429459
16	0.008915876360257716
17	0.01783175272051543
18	0.003988681529588979
19	0.005396451481208618
20	0.005161823155938678
21	0.004927194830668738
22	0.005865708131748497
23	0.007977363059177957
24	0.010792902962417236
25	0.013608442865656512
26	0.01665861109416573
27	0.007742734733908016
28	0.004927194830668738
29	0.008681248034987776
30	0.0028155399032392787
31	0.004927194830668738
32	0.005865708131748497
33	0.0063349647822883775
34	0.010558274637147294
35	0.013843071190926454
36	0.011731416263496995
37	0.007977363059177957
38	0.004457938180128858
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	426206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.70033964198217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06607702841895	48.76006934295244
2	10.900145277005006	12.796844598142831
3	2.7298983790521794	4.807378861153785
4	1.08260579884906	2.54197312363277
5	0.5384381719609271	1.5803251785157206
6	0.30776266701361404	1.0839463849692845
7	0.18853364217998764	0.7746892180933646
8	0.15901819746168003	0.7467537760205123
9	0.1080426869792652	0.5707928179363708
>10	0.7096617751377485	8.26048192948038
>50	0.12092470522019273	4.951901737184731
>100	0.08448712847844711	9.609763742119611
>500	0.002002064655887372	0.8294693957671545
>1k	0.0024024775870648465	2.685609894031031
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2831	0.6642327888391998	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2458	0.5767164235135123	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2071	0.4859152616340455	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1898	0.4453245613623459	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1119	0.2625490959770627	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.24612511320816696	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	963	0.2259470772349521	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	903	0.2118693777187557	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	637	0.14945824319695172	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	519	0.1217721008150988	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	507	0.11895656091185952	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	497	0.11661027765916011	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	483	0.11332548110538096	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	457	0.10722514464836252	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	457	0.10722514464836252	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	444	0.10417497641985331	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	443	0.10394034809458337	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.10300183479350362	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	428	0.10042092321553428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6925665053987976E-4
2	0.0	0.0	0.0	0.0	4.6925665053987976E-4
3	0.0	0.0	0.0	0.0	4.6925665053987976E-4
4	0.0	0.0	0.0	0.0	4.6925665053987976E-4
5	0.0	0.0	0.0	0.0	4.6925665053987976E-4
6	0.0	0.0	0.0	0.0	9.385133010797595E-4
7	0.0	0.0	0.0	0.0	9.385133010797595E-4
8	0.0	0.0	0.0	0.0	9.385133010797595E-4
9	0.0	0.0	0.0	0.0	9.385133010797595E-4
10	0.0	0.0	0.0	0.0	0.0011731416263496995
11	0.0	0.0	0.0	2.3462832526993988E-4	0.0011731416263496995
12	0.0	0.0	0.0	2.3462832526993988E-4	0.002111654927429459
13	0.0	0.0	0.0	2.3462832526993988E-4	0.002111654927429459
14	0.0	0.0	0.0	2.3462832526993988E-4	0.002111654927429459
15	0.0	0.0	0.0	2.3462832526993988E-4	0.002111654927429459
16	2.3462832526993988E-4	0.0	0.0	4.6925665053987976E-4	0.002111654927429459
17	2.3462832526993988E-4	0.0	0.0	4.6925665053987976E-4	0.002111654927429459
18	2.3462832526993988E-4	0.0	0.0	9.385133010797595E-4	0.002111654927429459
19	2.3462832526993988E-4	0.0	0.0	0.0011731416263496995	0.002111654927429459
20	2.3462832526993988E-4	0.0	0.0	0.001877026602159519	0.002111654927429459
21	2.3462832526993988E-4	0.0	0.0	0.0035194248790490984	0.002111654927429459
22	2.3462832526993988E-4	0.0	0.0	0.004457938180128858	0.002111654927429459
23	2.3462832526993988E-4	0.0	0.0	0.007038849758098197	0.002111654927429459
24	2.3462832526993988E-4	0.0	0.0	0.011027531287687175	0.002111654927429459
25	2.3462832526993988E-4	0.0	0.0	0.014077699516196394	0.002111654927429459
26	2.3462832526993988E-4	0.0	0.0	0.01736249606997555	0.002111654927429459
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCAA	40	1.590466E-4	24.00176	11
GTACAGG	55	5.7875037E-5	20.379482	1
GTCTAGA	40	0.004458811	20.015562	1
GTCTTAA	40	0.004458811	20.015562	1
CACGTGC	40	0.0044831145	19.996775	8
TATACTG	40	0.0044861604	19.994427	5
CGAATGT	60	1.13540904E-4	18.668036	23
CGCTGAG	115	9.349606E-10	18.088284	12
CTATAGA	45	0.00880186	17.79161	1
AGCATAA	45	0.008837656	17.779081	22
TCGCTCG	45	0.008849615	17.774912	19
GCTCTTA	155	0.0	17.56204	1
ACACGCT	120	1.6480044E-9	17.334604	9
CACGCTG	120	1.6480044E-9	17.334604	10
ACGCTGA	120	1.6480044E-9	17.334604	11
TAGCGGC	140	4.0017767E-11	17.144115	30
CTAGCGG	140	4.0017767E-11	17.144115	29
GCGAGAA	75	3.2239506E-5	17.067919	20
AGGGGCG	75	3.2239506E-5	17.067919	32
CATCGTT	95	7.6680226E-7	16.841364	28
>>END_MODULE