FastQCFastQC Report
Thu 2 Feb 2017
SRR4063070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426206
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35390.8303496431303172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27200.6381890447342364No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24430.5731969986344632No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23820.5588846707929968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14780.3467806647489712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.2953970615148543No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10100.2369746085226393No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7940.18629489026433227No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6970.1635359427131481No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC6340.14875435822114189No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.14195013678831364No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.13843071190926454No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5830.13678831363237495No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5610.13162649047643626No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.1271685522963074No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG4900.11496787938227054No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4870.11426399440646073No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4550.10675588799782265No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC4520.10605200302201284No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4440.10417497641985331No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA4440.10417497641985331No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4430.10394034809458337No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT4430.10394034809458337No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG4400.10323646311877356No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC4360.10229794981769379No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4340.10182869316715391No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG4330.10159406484188398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCC200.003747588732.005753
TACACTT401.58932E-424.0043135
ATATACC350.002062264822.8612523
GTATATA350.002063672522.8585681
TAGGACG604.7017347E-621.3371684
AGGGACG1203.6379788E-1220.0035958
GATGGAT400.004474275720.0035933
TAGGCAT400.004474275720.0035935
TACACGG400.004474275720.0035935
GCGAATG801.2615601E-719.99890114
CTTATAC659.396403E-619.6958473
CGAATGT902.0305379E-819.5544823
GACGGCC1154.5474735E-1119.4771911
TATACTG507.1770954E-419.2034515
CCTTTAG601.13550595E-418.667831
GGCGTTA601.13652844E-418.6656431
TTAGGCA450.00883224717.7809734
AGGGATC450.00883224717.7809735
ATTCGTT450.00884419717.776832
GCATTCG1352.3646862E-1117.776822
Produced by FastQC (version 0.11.5)