##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426206
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.047305762940926	32.0	32.0	32.0	32.0	32.0
2	31.305382843038345	32.0	32.0	32.0	32.0	32.0
3	31.39692308414241	32.0	32.0	32.0	32.0	32.0
4	31.49466455188336	32.0	32.0	32.0	32.0	32.0
5	31.408689694654697	32.0	32.0	32.0	32.0	32.0
6	34.92154732687949	36.0	36.0	36.0	36.0	36.0
7	34.96717315101148	36.0	36.0	36.0	36.0	36.0
8	34.911146253220274	36.0	36.0	36.0	36.0	36.0
9	34.982834591723254	36.0	36.0	36.0	36.0	36.0
10	34.860154479289356	36.0	36.0	36.0	32.0	36.0
11	35.03127360947523	36.0	36.0	36.0	36.0	36.0
12	34.92947307170711	36.0	36.0	36.0	36.0	36.0
13	34.972989587194924	36.0	36.0	36.0	36.0	36.0
14	34.914313735611415	36.0	36.0	36.0	32.0	36.0
15	34.88687160668785	36.0	36.0	36.0	32.0	36.0
16	34.88774442405785	36.0	36.0	36.0	32.0	36.0
17	34.85252671243483	36.0	36.0	36.0	32.0	36.0
18	34.841956706381424	36.0	36.0	36.0	32.0	36.0
19	34.82839753546407	36.0	36.0	36.0	32.0	36.0
20	34.81657930672022	36.0	36.0	36.0	32.0	36.0
21	34.77910212432486	36.0	36.0	36.0	32.0	36.0
22	34.7867040820636	36.0	36.0	36.0	32.0	36.0
23	34.692486731768206	36.0	36.0	36.0	32.0	36.0
24	34.68652013345659	36.0	36.0	36.0	32.0	36.0
25	34.670025762190114	36.0	36.0	36.0	32.0	36.0
26	34.60062974242503	36.0	36.0	36.0	32.0	36.0
27	34.56903469214417	36.0	36.0	36.0	32.0	36.0
28	34.5205604801434	36.0	36.0	36.0	32.0	36.0
29	34.47520682486873	36.0	36.0	36.0	32.0	36.0
30	34.442846886247494	36.0	36.0	36.0	32.0	36.0
31	34.42561578203967	36.0	36.0	36.0	32.0	36.0
32	34.3658864492757	36.0	36.0	36.0	32.0	36.0
33	34.29628161030112	36.0	36.0	36.0	32.0	36.0
34	34.264559860724624	36.0	36.0	36.0	32.0	36.0
35	34.17862958287776	36.0	36.0	36.0	32.0	36.0
36	34.13531484774968	36.0	36.0	36.0	32.0	36.0
37	34.100568739060456	36.0	36.0	36.0	32.0	36.0
38	33.64283233929133	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	13.0
22	35.0
23	108.0
24	264.0
25	705.0
26	1587.0
27	3094.0
28	5501.0
29	8612.0
30	13040.0
31	18535.0
32	26016.0
33	40796.0
34	95019.0
35	212878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14128402048914	18.23373856402387	12.610957419100515	25.014019996386477
2	14.686325391946617	21.89692308414241	37.43706095174634	25.979690572164632
3	18.176421730336973	26.795024002477675	28.905271159955515	26.123283107229838
4	11.704199377765681	16.69521311290784	36.203619845802265	35.39696766352421
5	13.084752443654008	38.19491044236825	33.29375935580447	15.426577758173277
6	32.49891484144954	36.19431963491747	17.453572811205873	13.853192712427118
7	28.194882744219328	31.586677772433454	21.928883987752375	18.289555495594843
8	28.362638961233984	32.58180470110136	20.185453709308817	18.870102628355834
9	27.67762659289658	14.218105112989775	18.77922705654064	39.325041237573
10	15.68970654828802	27.473058612914503	31.908738324225776	24.928496514571695
11	36.53957609361325	21.779580880334322	22.26623085604875	19.414612170003682
12	24.12078694524935	24.65926021515468	29.814056615947727	21.405896223648245
13	30.274469502867174	20.01342080318345	25.70553068483637	24.006579009113008
14	23.950390186904926	19.967808993772966	26.214318897434573	29.86748192188754
15	24.928790303280575	26.92923140453208	24.87482578846849	23.267152503718858
16	24.50528617616833	25.976405775610857	25.753508866604413	23.764799181616404
17	22.823939597283943	25.749050928424282	27.317775911179098	24.10923356311267
18	24.113222244642262	24.03673341060426	29.108224661314015	22.741819683439463
19	24.268780824296233	25.905313393054065	27.423358657550573	22.402547125099133
20	24.599841391252117	23.750252225449664	28.418652013345657	23.23125436995256
21	25.91258687113743	24.62705827698343	25.88349295880396	23.57686189307518
22	24.297170851653895	24.785901653191182	26.85274257049408	24.064184924660843
23	22.881946481153438	24.46803768139745	27.238066188805853	25.411949648643255
24	23.279822433283435	26.290338474822033	27.01393222995453	23.41590686194
25	24.418004439167916	24.432082138684112	26.8830096244539	24.266903797694074
26	22.83614027019798	25.37622651957035	28.046296861142267	23.741336349089405
27	24.46146807287573	25.463568001313924	27.122628781924192	22.952335143886156
28	23.333317691444982	24.526402725442626	27.970511912080077	24.169767671032318
29	22.767863427544427	25.349244262164305	28.33958226772969	23.543310042561576
30	23.635284346067394	25.18406592117427	28.271774681726676	22.90887505103166
31	23.08226538340615	25.220433311591105	27.185210907401586	24.51209039760116
32	22.724926444020028	26.067910822466132	27.103325621882373	24.103837111631464
33	22.654537946439046	25.37247246636603	27.560381599508222	24.412607987686705
34	23.081561498430336	25.812165947921894	28.04066578133579	23.06560677231198
35	24.527106610418436	25.423152184624335	27.340065602079743	22.709675602877482
36	23.18714424480181	26.53294416315115	26.869635809913518	23.41027578213352
37	24.214581681158876	26.271098952149902	27.03223323932559	22.48208612736564
38	23.404934245257564	25.926725402095236	26.71906711558992	23.949273237057284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	270.0
1	221.5
2	173.0
3	173.0
4	676.0
5	1179.0
6	1179.0
7	1317.0
8	1455.0
9	1391.5
10	1328.0
11	1328.0
12	1621.0
13	1914.0
14	2249.0
15	2584.0
16	2584.0
17	3770.0
18	4956.0
19	4956.0
20	5263.0
21	5570.0
22	5151.0
23	4732.0
24	4732.0
25	5011.5
26	5291.0
27	5291.0
28	6880.0
29	8469.0
30	10124.5
31	11780.0
32	11780.0
33	13940.0
34	16100.0
35	16100.0
36	17308.5
37	18517.0
38	21050.5
39	23584.0
40	23584.0
41	24833.5
42	26083.0
43	29376.5
44	32670.0
45	32670.0
46	38086.5
47	43503.0
48	43503.0
49	42530.5
50	41558.0
51	38661.5
52	35765.0
53	35765.0
54	33571.0
55	31377.0
56	31377.0
57	30358.5
58	29340.0
59	26192.5
60	23045.0
61	23045.0
62	21745.0
63	20445.0
64	16861.5
65	13278.0
66	13278.0
67	10943.0
68	8608.0
69	8608.0
70	6857.0
71	5106.0
72	3989.5
73	2873.0
74	2873.0
75	2144.0
76	1415.0
77	1415.0
78	1437.5
79	1460.0
80	1093.5
81	727.0
82	727.0
83	707.5
84	688.0
85	688.0
86	429.0
87	170.0
88	156.0
89	142.0
90	142.0
91	83.0
92	24.0
93	19.5
94	15.0
95	15.0
96	12.0
97	9.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006804221432828256
2	0.0
3	0.0
4	0.0
5	0.0
6	2.346283252699399E-4
7	2.346283252699399E-4
8	0.0018770266021595193
9	0.003988681529588979
10	7.038849758098197E-4
11	0.0063349647822883775
12	2.346283252699399E-4
13	4.692566505398798E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.346283252699399E-4
24	0.0
25	0.0
26	0.0
27	2.346283252699399E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.346283252699399E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	426206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.195561711798305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.72973192042929	45.92848193823216
2	11.682535963253718	13.130133413466549
3	3.0488434997455998	5.13994419118707
4	1.1660309599880732	2.6210305907950873
5	0.5873878081475513	1.650429391075683
6	0.33510648632916107	1.129889833952056
7	0.20554274550927454	0.8085413027775225
8	0.14142823824475284	0.6358111432059138
9	0.11949909604322272	0.6043786943580931
>10	0.7612919849843762	8.666676720205485
>50	0.12492502185143654	5.008864697833697
>100	0.09138810323276797	10.002652387761636
>500	0.003353691861752953	1.1793047068566824
>1k	0.0029344803790338336	3.493860988292341
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3539	0.8303496431303172	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2720	0.6381890447342364	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2443	0.5731969986344632	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2382	0.5588846707929968	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1478	0.3467806647489712	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.2953970615148543	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1010	0.2369746085226393	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	794	0.18629489026433227	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	697	0.1635359427131481	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	634	0.14875435822114189	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	605	0.14195013678831364	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.13843071190926454	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	583	0.13678831363237495	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	561	0.13162649047643626	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	542	0.1271685522963074	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	490	0.11496787938227054	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	487	0.11426399440646073	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	455	0.10675588799782265	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	452	0.10605200302201284	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	444	0.10417497641985331	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	444	0.10417497641985331	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	443	0.10394034809458337	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	443	0.10394034809458337	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	440	0.10323646311877356	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	436	0.10229794981769379	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	434	0.10182869316715391	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	433	0.10159406484188398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.3462832526993988E-4	0.0
12	0.0	0.0	0.0	2.3462832526993988E-4	4.6925665053987976E-4
13	0.0	0.0	0.0	2.3462832526993988E-4	4.6925665053987976E-4
14	0.0	0.0	0.0	2.3462832526993988E-4	4.6925665053987976E-4
15	0.0	0.0	0.0	2.3462832526993988E-4	4.6925665053987976E-4
16	0.0	0.0	0.0	4.6925665053987976E-4	4.6925665053987976E-4
17	0.0	0.0	0.0	4.6925665053987976E-4	4.6925665053987976E-4
18	0.0	0.0	0.0	9.385133010797595E-4	4.6925665053987976E-4
19	0.0	0.0	0.0	0.0014077699516196393	4.6925665053987976E-4
20	0.0	0.0	0.0	0.002111654927429459	4.6925665053987976E-4
21	0.0	0.0	0.0	0.003754053204319038	4.6925665053987976E-4
22	0.0	0.0	0.0	0.004927194830668738	4.6925665053987976E-4
23	0.0	0.0	0.0	0.007508106408638076	4.6925665053987976E-4
24	0.0	0.0	0.0	0.011496787938227055	4.6925665053987976E-4
25	0.0	0.0	0.0	0.014781584492006212	4.6925665053987976E-4
26	0.0	0.0	0.0	0.01783175272051543	4.6925665053987976E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATCC	20	0.0037475887	32.00575	3
TACACTT	40	1.58932E-4	24.004313	5
ATATACC	35	0.0020622648	22.861252	3
GTATATA	35	0.0020636725	22.858568	1
TAGGACG	60	4.7017347E-6	21.337168	4
AGGGACG	120	3.6379788E-12	20.003595	8
GATGGAT	40	0.0044742757	20.003593	3
TAGGCAT	40	0.0044742757	20.003593	5
TACACGG	40	0.0044742757	20.003593	5
GCGAATG	80	1.2615601E-7	19.998901	14
CTTATAC	65	9.396403E-6	19.695847	3
CGAATGT	90	2.0305379E-8	19.55448	23
GACGGCC	115	4.5474735E-11	19.47719	11
TATACTG	50	7.1770954E-4	19.203451	5
CCTTTAG	60	1.13550595E-4	18.66783	1
GGCGTTA	60	1.13652844E-4	18.66564	31
TTAGGCA	45	0.008832247	17.780973	4
AGGGATC	45	0.008832247	17.780973	5
ATTCGTT	45	0.008844197	17.7768	32
GCATTCG	135	2.3646862E-11	17.7768	22
>>END_MODULE