FastQCFastQC Report
Thu 2 Feb 2017
SRR4063069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090081
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27620.2533756665789056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18430.1690700048895449No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11540.10586369269806556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11160.1023777132158069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG350.002067454222.8552764
CGAGCCG2800.020.57163415
TCGCGTA852.3966277E-718.8228559
GCGTAAC953.79041E-818.52565211
CGCCGGT2500.016.6386417
AGGCCCG3700.016.43184310
ATACCGC3700.016.43109127
TCTAGCG2250.016.35496928
GTTCAAA4550.016.1893561
GTCTTAC703.6727203E-416.0133841
CGTCTTA805.6199497E-516.0001632
CTAGCGG2300.016.00015829
CAGCGTA600.002442380415.9994289
CGGTCCA2700.015.99942710
CGCGTAA908.582903E-615.99942710
TACCGCA3700.015.99942628
ATACCGT2100.015.9979586
AAGACGG2650.015.6961125
CCGAGCC3700.015.56772314
CGTCGTA1850.015.56700910
Produced by FastQC (version 0.11.5)