##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063069_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090081
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211373283269776	32.0	32.0	32.0	32.0	32.0
2	30.81494127500617	32.0	32.0	32.0	32.0	32.0
3	30.8633936377205	32.0	32.0	32.0	32.0	32.0
4	30.936112087083437	32.0	32.0	32.0	32.0	32.0
5	30.839860524126188	32.0	32.0	32.0	32.0	32.0
6	34.48220636815062	36.0	36.0	36.0	32.0	36.0
7	34.420926518304604	36.0	36.0	36.0	32.0	36.0
8	34.39721818837316	36.0	36.0	36.0	32.0	36.0
9	34.541586359178815	36.0	36.0	36.0	32.0	36.0
10	34.2627410256669	36.0	36.0	36.0	32.0	36.0
11	34.54308349563014	36.0	36.0	36.0	32.0	36.0
12	34.36790110092736	36.0	36.0	36.0	32.0	36.0
13	34.43388518834839	36.0	36.0	36.0	32.0	36.0
14	34.341438847204934	36.0	36.0	36.0	32.0	36.0
15	34.29562940735597	36.0	36.0	36.0	32.0	36.0
16	34.30874953329156	36.0	36.0	36.0	32.0	36.0
17	34.23223044892994	36.0	36.0	36.0	32.0	36.0
18	34.2630079783062	36.0	36.0	36.0	32.0	36.0
19	34.25378389312354	36.0	36.0	36.0	32.0	36.0
20	34.241281152501514	36.0	36.0	36.0	32.0	36.0
21	34.22533187900716	36.0	36.0	36.0	32.0	36.0
22	34.20149970506779	36.0	36.0	36.0	32.0	36.0
23	34.147703702752366	36.0	36.0	36.0	32.0	36.0
24	34.13269839580728	36.0	36.0	36.0	32.0	36.0
25	34.110092736227855	36.0	36.0	36.0	32.0	36.0
26	34.05657286018195	36.0	36.0	36.0	32.0	36.0
27	34.07153963788012	36.0	36.0	36.0	32.0	36.0
28	34.046473610676635	36.0	36.0	36.0	32.0	36.0
29	34.02398904301607	36.0	36.0	36.0	32.0	36.0
30	33.9948847837913	36.0	36.0	36.0	32.0	36.0
31	34.01383750381852	36.0	36.0	36.0	32.0	36.0
32	33.97181493852292	36.0	36.0	36.0	32.0	36.0
33	33.93599374725365	36.0	36.0	36.0	32.0	36.0
34	33.93210321067884	36.0	36.0	36.0	32.0	36.0
35	33.90291363669305	36.0	36.0	36.0	32.0	36.0
36	33.842192460927215	36.0	36.0	36.0	32.0	36.0
37	33.85132572717073	36.0	36.0	36.0	32.0	36.0
38	33.26213648343563	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	9.0
12	3.0
13	5.0
14	87.0
15	225.0
16	295.0
17	342.0
18	483.0
19	674.0
20	938.0
21	1270.0
22	2090.0
23	3071.0
24	4937.0
25	7459.0
26	10745.0
27	15720.0
28	21914.0
29	29496.0
30	39949.0
31	53286.0
32	72275.0
33	106950.0
34	232310.0
35	485536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76960804123484	17.011116948386647	11.307159887875326	25.912115122503188
2	16.586598034662345	19.39261043321505	37.92407768095297	26.09671385116964
3	19.188622847884446	23.412943794989605	28.40535645804395	28.993076899082
4	12.544573357173064	15.075174967914522	36.160127406181516	36.2201242687309
5	14.477744752678703	36.53291501541172	34.04337296345222	14.94596726845736
6	34.57024674358464	35.44633003663037	16.67447955606887	13.308943663716116
7	30.300684077605244	30.39994275654745	20.792491567140424	18.506881598706883
8	28.050444050385337	33.27942286751694	19.383182319813518	19.286950762284203
9	27.197611920582048	14.398746515167222	18.55027286963079	39.85336869461994
10	15.430518861001028	27.03122706590342	32.257357258182886	25.28089681491267
11	37.46171099888539	21.381110300761883	22.05097861136721	19.10620008898552
12	24.278343800627855	23.79754546071691	29.190357979637856	22.733752759017378
13	29.571536047855844	19.604198396241994	25.1069783657816	25.717287190120558
14	23.223882787915485	19.62458371177717	25.6064734538849	31.545060046422446
15	24.898838175949088	27.23560749395782	22.71622020887508	25.149334121218015
16	25.468135586378242	26.084028617511436	23.9576242107523	24.490211585358022
17	23.892277159406937	26.058588585254583	25.475767315581386	24.57336693975709
18	24.59221530298082	25.140637556924727	26.34279968734978	23.92434745274468
19	25.11087135619935	25.42958636773143	25.775916007493567	23.68362626857565
20	25.371413158952173	24.616385297231442	25.542968699829178	24.469232843987207
21	26.230016531715755	24.662347503637527	25.00472465794394	24.102911306702776
22	25.304356122810333	24.57463536897962	25.52481362487913	24.59619488333092
23	24.128060595384888	24.53219496799953	25.850104221713345	25.489640214902238
24	24.233043558042986	25.24081932243044	25.780856480950526	24.745280638576048
25	24.6476527770769	24.812529532390435	25.693064991902965	24.846752698629697
26	24.257446636718637	25.546569922781053	26.03423471035556	24.16174873014475
27	24.97669716219142	25.139266566115836	25.35036825823214	24.5336680134606
28	24.31675690949357	24.97720229499955	25.994154246428053	24.711886549078827
29	24.13055345306085	25.401775270075	25.987660267437327	24.48001100942682
30	24.389138569563666	25.260559290827377	26.066112017789862	24.284190121819094
31	24.5102896061767	25.29678027801019	25.24806611182057	24.944864003992542
32	24.330654483403976	25.265774939909356	25.238160767692335	25.165409808994333
33	24.094741892837842	25.013004670672196	25.655852835752157	25.236400600737802
34	24.735977276292864	25.160762337804126	25.755644916387073	24.347615469515937
35	25.171916129701145	24.945936448604993	25.77480386193128	24.10734355976259
36	24.249077460304644	25.56641460062426	25.51420922154348	24.67029871752761
37	25.02942690247333	25.34887966759298	25.40768760051707	24.214005829416628
38	24.260224746726458	25.305886918397434	25.702115635667738	24.731772699208367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	73.0
2	95.0
3	95.0
4	310.5
5	526.0
6	526.0
7	682.5
8	839.0
9	844.0
10	849.0
11	849.0
12	1144.5
13	1440.0
14	2005.0
15	2570.0
16	2570.0
17	3702.0
18	4834.0
19	4834.0
20	6041.0
21	7248.0
22	8015.5
23	8783.0
24	8783.0
25	10565.0
26	12347.0
27	12347.0
28	15451.5
29	18556.0
30	22213.5
31	25871.0
32	25871.0
33	31873.0
34	37875.0
35	37875.0
36	42576.0
37	47277.0
38	53754.0
39	60231.0
40	60231.0
41	65698.0
42	71165.0
43	79717.5
44	88270.0
45	88270.0
46	92332.0
47	96394.0
48	96394.0
49	100188.0
50	103982.0
51	104034.0
52	104086.0
53	104086.0
54	99027.5
55	93969.0
56	93969.0
57	89729.0
58	85489.0
59	75910.5
60	66332.0
61	66332.0
62	61067.0
63	55802.0
64	46029.5
65	36257.0
66	36257.0
67	30048.5
68	23840.0
69	23840.0
70	19048.5
71	14257.0
72	10997.0
73	7737.0
74	7737.0
75	5875.5
76	4014.0
77	4014.0
78	3713.5
79	3413.0
80	2630.5
81	1848.0
82	1848.0
83	1857.5
84	1867.0
85	1867.0
86	1231.0
87	595.0
88	504.0
89	413.0
90	413.0
91	315.5
92	218.0
93	183.5
94	149.0
95	149.0
96	136.5
97	124.0
98	124.0
99	296.0
100	468.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11861503869895908
2	0.05495004499665621
3	0.009815784331623063
4	0.0022016712519528363
5	9.173630216470152E-5
6	1.8347260432940304E-4
7	0.0
8	1.8347260432940304E-4
9	0.0
10	9.173630216470152E-5
11	0.0014677808346352243
12	0.0017429897411293288
13	0.012934818605222914
14	0.00834800349698784
15	0.02229192142602247
16	0.010916619957599481
17	0.019264623454587317
18	0.004128133597411568
19	0.00724716787101142
20	0.005045496619058584
21	0.005045496619058584
22	0.006880222662352615
23	0.008898421309976048
24	0.014310863137693438
25	0.01614558918098747
26	0.017705106317787395
27	0.007797585683999629
28	0.00541244182771739
29	0.009724048029458362
30	0.0020181986476234337
31	0.004495078806070374
32	0.005595914432046793
33	0.008072794590493735
34	0.01082488365543478
35	0.014677808346352244
36	0.014219126835528735
37	0.00843973979915254
38	0.004953760316893882
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1090081.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.37626763916171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.26560455785418	51.50310704316846
2	11.979603310911273	15.184450912866012
3	3.3429303383156044	6.355873434604894
4	1.2773070144004834	3.238038048080945
5	0.6192460142848332	1.9622750567899871
6	0.3615187876700937	1.374702686637908
7	0.23112423057597026	1.025345376641461
8	0.14698063227520686	0.7452067111077377
9	0.10822611822216975	0.6173070690596207
>10	0.5583585980449758	6.39353922719017
>50	0.05158787329118639	2.2943582759877437
>100	0.05184841448982703	6.478216056805815
>500	0.004937941758552338	2.095649210316283
>1k	7.261679056694615E-4	0.7319308907429322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2762	0.2533756665789056	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1843	0.1690700048895449	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1154	0.10586369269806556	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1116	0.1023777132158069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.173630216470152E-5
2	0.0	0.0	0.0	0.0	9.173630216470152E-5
3	0.0	0.0	0.0	0.0	9.173630216470152E-5
4	0.0	0.0	0.0	0.0	9.173630216470152E-5
5	0.0	0.0	0.0	0.0	9.173630216470152E-5
6	0.0	0.0	0.0	0.0	1.8347260432940304E-4
7	0.0	0.0	0.0	0.0	1.8347260432940304E-4
8	0.0	0.0	0.0	0.0	1.8347260432940304E-4
9	0.0	0.0	0.0	0.0	1.8347260432940304E-4
10	0.0	0.0	0.0	1.8347260432940304E-4	1.8347260432940304E-4
11	0.0	0.0	0.0	1.8347260432940304E-4	1.8347260432940304E-4
12	0.0	0.0	0.0	1.8347260432940304E-4	3.669452086588061E-4
13	0.0	0.0	0.0	1.8347260432940304E-4	5.504178129882092E-4
14	0.0	0.0	0.0	1.8347260432940304E-4	5.504178129882092E-4
15	0.0	0.0	0.0	1.8347260432940304E-4	6.421541151529107E-4
16	9.173630216470152E-5	0.0	0.0	2.752089064941046E-4	8.256267194823137E-4
17	9.173630216470152E-5	0.0	0.0	2.752089064941046E-4	8.256267194823137E-4
18	9.173630216470152E-5	0.0	0.0	3.669452086588061E-4	9.173630216470153E-4
19	9.173630216470152E-5	0.0	0.0	5.504178129882092E-4	9.173630216470153E-4
20	9.173630216470152E-5	0.0	0.0	5.504178129882092E-4	0.0011008356259764184
21	9.173630216470152E-5	0.0	0.0	6.421541151529107E-4	0.0011925719281411199
22	9.173630216470152E-5	0.0	0.0	8.256267194823137E-4	0.0011925719281411199
23	9.173630216470152E-5	0.0	0.0	0.0012843082303058214	0.0011925719281411199
24	9.173630216470152E-5	0.0	0.0	0.0018347260432940305	0.0011925719281411199
25	9.173630216470152E-5	0.0	0.0	0.0022934075541175382	0.0011925719281411199
26	9.173630216470152E-5	0.0	0.0	0.003394243180093956	0.0011925719281411199
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	35	0.0020674542	22.855276	4
CGAGCCG	280	0.0	20.571634	15
TCGCGTA	85	2.3966277E-7	18.822855	9
GCGTAAC	95	3.79041E-8	18.525652	11
CGCCGGT	250	0.0	16.638641	7
AGGCCCG	370	0.0	16.431843	10
ATACCGC	370	0.0	16.431091	27
TCTAGCG	225	0.0	16.354969	28
GTTCAAA	455	0.0	16.189356	1
GTCTTAC	70	3.6727203E-4	16.013384	1
CGTCTTA	80	5.6199497E-5	16.00016	32
CTAGCGG	230	0.0	16.000158	29
CAGCGTA	60	0.0024423804	15.999428	9
CGGTCCA	270	0.0	15.999427	10
CGCGTAA	90	8.582903E-6	15.999427	10
TACCGCA	370	0.0	15.999426	28
ATACCGT	210	0.0	15.997958	6
AAGACGG	265	0.0	15.696112	5
CCGAGCC	370	0.0	15.567723	14
CGTCGTA	185	0.0	15.567009	10
>>END_MODULE